A Study of Fermentation for Human Interleukin-11 with Recombinant E. Coli

2005 ◽  
Vol 3 (1) ◽  
Author(s):  
Kaili Zhao ◽  
Yiru Gan ◽  
Zisheng Jason Zhang
Keyword(s):  
Carbon Source ◽  
Batch Fermentation ◽  
Carbon Sources ◽  
Biological Properties ◽  
Operational Parameters ◽  
Fed Batch ◽  
E Coli ◽  
Dry Cell Weight ◽  
Fed Batch Fermentation ◽  
Interleukin 11

Recombinant human interleukin-11(rhIL-11) is still the only therapy drug for thrombocytopenia. Although the biological properties and clinical behaviors of rhIL-11 have been studied extensively, its bioprocess development data have rarely been reported. In this work, fermentation conditions for recombinant E. coli, which expressed interleukin-11 as a fusion protein, were investigated. Batch and fed-batch fermentation experiments were performed in shake flasks and in a BioFlo fermenter with different medium and carbon sources under different operational parameters. Based on the results of the study, M9Ca medium was shown as the better medium and glycerol was identified to be a better carbon source than glucose for the expression of rhIL-11. When glycerol was used as the carbon source in fed-batch fermentation, a dry cell weight of 9.2 g/L and a rhIL-11 expression of 22%, were achieved. The results could be used as references in the optimization of the fermentation process.

scholarly journals Differential expression of small RNAs under chemical stress and fed-batch fermentation in E. coli

BMC Genomics ◽  
2015 ◽  
Vol 16 (1) ◽  
Author(s):  
Martin Holm Rau ◽  
Klara Bojanovič ◽  
Alex Toftgaard Nielsen ◽  
Katherine S. Long
Keyword(s):  
Differential Expression ◽  
Small Rnas ◽  
Batch Fermentation ◽  
Chemical Stress ◽  
Fed Batch ◽  
E Coli ◽  
Fed Batch Fermentation

Co-Expression of Threonine Dehydratase and Acetolactate Synthase in Escherichia Coli for L-Isoleucine Production

2014 ◽  
Vol 989-994 ◽  
pp. 997-1002 ◽  
Author(s):  
Jian Wang ◽  
Jia Kai Sun ◽  
Qing Yang Xu
Keyword(s):  
Escherichia Coli ◽  
Corynebacterium Glutamicum ◽  
Carbon Flux ◽  
Batch Fermentation ◽  
Acetolactate Synthase ◽  
Fed Batch ◽  
Threonine Dehydratase ◽  
E Coli ◽  
Fed Batch Fermentation ◽  
Fermentation Period

Metabolic engineering ofCorynebacterium glutamicumhas sought to divert carbon into L-isoleucine. However, the fermentation period of this strain is long. TheC.glutamicumYILW strain (LeuL, AHVr, SGr, Leu-MEr) was previously derived by repeated compound mutagenesis which could accumulate 20.2 g/L L-isoleucine in a 5-L jar fermentor. Overexpression of the threonine dehydratase gene (ilvA) fromCorynebacterium glutamicumYILW and coexpression of threonine dehydratase and acetolactate synthase (ilvBN) from it were employed to divert carbon flux toward L-isoleucine. The strainE. coliTRFC with the expression ofilvA could accumulate L-isoleucine of 6.8 g/L without accumulation of any L-threonine by fed-batch fermentation in a 5-L jar fermentor. However, the production of L-isoleucine by the strainE.coliTRFC with the co-expression ofilvA andilvBN was decreased by 19.1%, and the production of L-valine was increased by 40% compared with that ofE. coliTRFC with the expression ofilvA.

scholarly journals DIFFERENT FRUCTOSE FEEDING STRATEGIES FOR POLY(3-HYDROXYBUTYRATE) PRODUCTION BY Yangia sp. ND199

2017 ◽  
Vol 55 (2) ◽  
pp. 195
Author(s):  
Truong Huu Phong ◽  
Dang Minh Khuong ◽  
Duong Van Hop ◽  
Doan Van Thuoc
Keyword(s):  
Batch Fermentation ◽  
Halophilic Bacteria ◽  
Carbon Sources ◽  
Dry Weight ◽  
Halophilic Bacterium ◽  
Feeding Strategies ◽  
Fed Batch ◽  
Mangrove Soil ◽  
Fed Batch Fermentation ◽  
Maximum Cell

Yangia sp. ND199 is a halophilic bacterium isolated from mangrove soil sample. This strain was able to produce polyhydroxyalkanoate (PHA) from different carbon sources. Only homopolymer poly(3-hydroxybutyrate) (PHB) was synthesized when fructose was used as carbon source. The bacterium can accumulate high PHB content during exponential phase. Maximum cell dry weight (CDW) of 7.8 g/l and PHB content of 49 wt% were obtained after 27 h of cultivation in batch fermentation. High CDW and PHB content were achieved by using fed-batch fermentation with different fructose feeding strategies. The highest CDW of 78.5 g/l, PHB content of 67.5 wt%, and PHB productivity of 1 g/l/h were obtained by using two-stage fed-batch fermentation, is among the highest reported so far for PHB production by halophilic bacteria.         

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