Multicomponent quantitative analysis combined with antioxidant and alpha‐glucosidase inhibitory activities for the quality evaluation of
            Gastrodia elata
            from different regions

This study investigated alpha-glucosidase and angiotensin-converting enzyme inhibitory activities of Sa-med mushroom powder. Healthy snack had been developed by varying 3 levels of Sa-med mushroom powder at 10, 12 and 14 % (w/w) and determined its physico-chemical properties and sensory evaluation. The results showed that anti-alpha-glucosidase activity and anti-angiotensin-converting activities of Sa-med mushroom powder were at 59 and 93 %, respectively. As the results of healthy snack, lightness (L*) and extended ratio were decreased, but redness (a*) and bulk density were increased with higher amounts of Sa-med mushroom powder in the formulation (p < 0.05). However, hardness and crispiness were not significantly different. For sensory evaluation, healthy snack with 14 % Sa-med mushroom powder had the highest overall liking score of 7.6 (like moderately to like very much). Finally, the nutritional values of developed snack revealed that protein and fiber were increased by 3 and 18 times, while carbohydrate was decreased by 15 %. In addition, the mushroom snack could be claimed as a “source of protein”, “high fiber” and “low sodium”. This snack also had phenolic compounds, antioxidant activities and alpha-glucosidase, and angiotensin-converting enzyme inhibitory activities. According to consumer acceptability test (n = 300), the acceptability level was 95 %, in which around 64 % of the subjects were interested in buying the product.

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Quality Evaluation of Fritillaria thunbergii by High-Performance Liquid Chromatography Fingerprinting Coupled with Multicomponent Quantitative Analysis

International Journal of Agriculture and Biology ◽

10.17957/ijab/15.1647 ◽

2021 ◽

Vol 25 (01) ◽

pp. 131-138

Author(s):

Ruoxu He

Keyword(s):

Quantitative Analysis ◽

High Performance ◽

Quality Evaluation ◽

Light Scattering Detection ◽

Mobile Phases ◽

Evaporative Light Scattering ◽

Significant Difference ◽

The Family ◽

Main Production ◽

Main Components

Medicinal Zhebeimu refers to the dried bulb of the plant Fritillaria thunbergii, which belongs to the family Liliaceae. Their main production sites are in Zhejiang, Jiangsu, and Anhui Provinces, with alkaloids as the main components. In this study, according to Chinese Pharmacopoeia, different mobile phases were investigated. Because most alkaloids in Fritillaria do not have UV absorption, evaporative light scattering detection (ELSD) was used instead of UV to produce the HPLC-ELSD spectra for five F. thunbergii samples and other samples, e.g. F. chuanxiensis, F. anhuiensis, and F. ussuriensis. Comparison showed significant differences exist among these species. There was no significant difference between the F. thunbergii samples produced in Zhejiang, which clustered together in both the cluster analyses. However, the F. thunbergii samples produced in Nantong, Jiangsu and the F. thunbergii samples produced in Zhejiang were separated in the clustering chart, which may be caused by different cultivation environments. The obtained results showed that the combination of quantitative analysis and chromatographic fingerprint analysis can be readily utilized for quality control purposes, offering a comprehensive strategy for quality evaluation of F. thunbergii and related products. © 2021 Friends Science Publishers

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