scholarly journals Quality Evaluation of Fritillaria thunbergii by High-Performance Liquid Chromatography Fingerprinting Coupled with Multicomponent Quantitative Analysis

2021 ◽  
Vol 25 (01) ◽  
pp. 131-138
Author(s):  
Ruoxu He
Keyword(s):  
Quantitative Analysis ◽  
High Performance ◽  
Quality Evaluation ◽  
Light Scattering Detection ◽  
Mobile Phases ◽  
Evaporative Light Scattering ◽  
Significant Difference ◽  
The Family ◽  
Main Production ◽  
Main Components

Medicinal Zhebeimu refers to the dried bulb of the plant Fritillaria thunbergii, which belongs to the family Liliaceae. Their main production sites are in Zhejiang, Jiangsu, and Anhui Provinces, with alkaloids as the main components. In this study, according to Chinese Pharmacopoeia, different mobile phases were investigated. Because most alkaloids in Fritillaria do not have UV absorption, evaporative light scattering detection (ELSD) was used instead of UV to produce the HPLC-ELSD spectra for five F. thunbergii samples and other samples, e.g. F. chuanxiensis, F. anhuiensis, and F. ussuriensis. Comparison showed significant differences exist among these species. There was no significant difference between the F. thunbergii samples produced in Zhejiang, which clustered together in both the cluster analyses. However, the F. thunbergii samples produced in Nantong, Jiangsu and the F. thunbergii samples produced in Zhejiang were separated in the clustering chart, which may be caused by different cultivation environments. The obtained results showed that the combination of quantitative analysis and chromatographic fingerprint analysis can be readily utilized for quality control purposes, offering a comprehensive strategy for quality evaluation of F. thunbergii and related products. © 2021 Friends Science Publishers

scholarly journals Chemical Fingerprint Analysis and Quantitative Analysis of Saccharides in Morindae Officinalis Radix by HPLC-ELSD

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7242
Author(s):  
Hongmei Sun ◽  
Yini Cai ◽  
Jie Shen ◽  
Enyao Ma ◽  
Zhimin Zhao ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Ion Mobility ◽  
High Performance ◽  
Gradient Elution ◽  
Fingerprint Analysis ◽  
Chemical Fingerprint ◽  
Hplc Fingerprint ◽  
Light Scattering Detection ◽  
Evaporative Light Scattering

A method based on high performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) was developed for the quantitative analysis of three active compounds and chemical fingerprint analyses of saccharides in Morindae officinalis radix. Ten batches of Morindae officinalis radix were collected from different plantations in the Guangdong region of China and used to establish the fingerprint. The samples were separated with a COSMOIL Sugar-D column (4.6 mm × 250 mm, 5 μm) by using gradient elution with water (A) and acetonitrile (B). In addition, Trapped-Ion-Mobility (tims) Time-Of-Flight (tims TOF) was used to identify saccharides of Morindae officinalis radix. Fingerprint chromatogram presented 26 common characteristic peaks in the roots of Morinda officinalis How, and the similarities were more than 0.926. In quantitative analysis, the three compounds showed good regression (r = 0.9995–0.9998) within the test ranges, and the recoveries of the method were in the range of 96.7–101.7%. The contents of sucrose, kestose and nystose in all samples were determined as 1.21–7.92%, 1.02–3.37%, and 2.38–6.55%, respectively. The developed HPLC fingerprint method is reliable and was validated for the quality control and identification of Morindae officinalis radix and can be successfully used to assess the quality of Morindae officinalis radix.

scholarly journals Determination of Triterpenoids and Phenolic Acids from Sanguisorba officinalis L. by HPLC-ELSD and Its Application

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4505
Author(s):  
Jiahui Sun ◽  
Chunli Gan ◽  
Jing Huang ◽  
Zhenyue Wang ◽  
Chengcui Wu ◽  
...  
Keyword(s):  
Phenolic Acids ◽  
High Performance ◽  
Gradient Elution ◽  
Light Scattering Detection ◽  
Evaporative Light Scattering ◽  
Acetic Acid Water ◽  
Sanguisorba Officinalis ◽  
Functional Components ◽  
Gradient Elution Mode

A novel analytical method involving high-performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) was developed for simultaneous determination of 11 phenolic acids and 12 triterpenes in Sanguisorba officinalis L. Chromatographic separation was conducted with gradient elution mode by using a DiamonsilTM C18 column (250 mm × 4.6 mm, 5 μm) with the mobile phase of 0.1% acetic acid water (A) and methanol (B). The drift tube temperature of ELSD was set at 70 °C and the nitrogen cumulative flow rate was 1.6 L/min. The method was fully validated to be linear over a wide concentration range (R2 ≥ 0.9991). The precisions (RSD) were less than 3.0% and the recoveries were between 97.7% and 101.4% for all compounds. The results indicated that this method is accurate and effective for the determination of 23 functional components in Sanguisorba officinalis L. and could also be successfully applied to study the influence of processing method on those functional components in Sanguisorba officinalis L.

Determination of mono- and diacylglycerols in edible oils and fats by high performance liquid chromatography and evaporative light scattering detector: Results of collaborative studies and the standardised method

1999 ◽  
Vol 71 (10) ◽  
pp. 1983-1991 ◽  
Author(s):  
D. Berner ◽  
A. Dieffenbacher
Keyword(s):  
Liquid Chromatography ◽  
Light Scattering ◽  
High Performance ◽  
Edible Oils ◽  
Collaborative Study ◽  
Evaporative Light Scattering Detector ◽  
Light Scattering Detection ◽  
Collaborative Studies ◽  
Evaporative Light Scattering

The development, by collaborative study, of standardised method for the determination of mono- and diacylglycerols in vegetable oils and fats is described. The method involves separation of mono- and diacylglycerols by normal phase high-performance liquid-liquid chromatography (HPLC) and evaporative light scattering detection of a solution of oil, fat or a commercial mono- and diacylglycerol preparation in a organic solvent.

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