Celecoxib enhances the therapeutic efficacy of epirubicin for Novikoff hepatoma in rats

The cellular uptake and lysosomal accumulation of 67Ga-labelled transferrin within tumors of different malignancy were examined using tissue fractionation and immunological techniques. As tumor models the slowly growing Morris hepatoma 5123C, the moderately growing Novikoff hepatoma and the fast and aggressive Yoshida hepatoma AH 130 were investigated. Isolation of subcellular fractions of tumor homogenates was performed by differential centrifugation and density-gradient centrifugation. The intracellular 67Gatransferrin was found to be highly concentrated within the purified lysosomes. The transferrin within the lysosomal fraction was identified by radial immunodiffusion technique using monospecific antiserum. The accumulation of 67Gatransferrin by the tumors resulted in a faster disappearance of 67Ga-transferrin from the blood. This loss of circulating 67Ga-transferrin correlated with the proliferation activity and the spread of the tumors. Since transferrin is indispensible for the utilization of iron by the heme-synthesizing red cell precursors, transferrin concentration in the blood is the limiting factor for the utilization of iron in hemoglobin synthesis. Thus, in a further series of experiments we investigated the development of anemia in tumor-bearing rats. With increasing tumor mass a progressive fall of hemoglobin concentration was found. The anemia was more severe in the faster growing Novikoff hepatoma than in the slowly growing Morris hepatoma. The most significant reduction of hemoglobin concentration was found in the very fast growing Yoshida hepatoma. After total tumor resection hemoglobin concentration and red blood cell count normalized completely within 6-8 weeks. We conclude from these data that the uptake of transferrin by the tumor cells results in a faster disappearance of transferrin from the blood. This loss of circulating transferrin correlates with tumor mass and proliferation activity and is one of the factors responsible for the anemia seen in patients with malignant tumors.

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Die Anämie bei malignen Tumorerkrankungen

Nuklearmedizin ◽

10.1055/s-0038-1629491 ◽

1989 ◽

Vol 28 (05) ◽

pp. 193-200 ◽

Cited By ~ 1

Author(s):

E. Aulbert

Keyword(s):

Cellular Uptake ◽

Tumor Tissue ◽

Malignant Tumors ◽

The Other ◽

Novikoff Hepatoma ◽

Cellular Accumulation ◽

Tumor Mass ◽

Proliferation Activity ◽

Morris Hepatoma ◽

Cellular Incorporation

Cellular uptake of 67Ga-labelled transferrin by the tumor tissue was studied in rats with tumors of different malignancy and different tumor mass using the slowly growing Morris hepatoma 5123C, the moderately growing Novikoff hepatoma and the very fast and aggressive Yoshida hepatoma AH130. The cellular accumulation of 67Ga-transferrin was found to correlate with the proliferation activity of the tumor. The 67Ga-transferrin concentration in the very fast growing Yoshida hepatoma was 4.8 times higher than the concentration in the slowly growing Morris hepatoma. The uptake of 67Ga-transferrin by the tumors resulted in a faster disappearance of circulating 67Ga-transferrin from the blood. The rate of disappearance correlated with the proliferation activity and the spread of the tumors. Using tumors of identical size the elimination of 67Ga-transferrin from the blood was much faster in the rats with Yoshida hepatoma than in those with the slowly growing Morris hepatoma. On the other hand, using tumors of different tumor size it could be demonstrated that the rate of disappearance of 67Ga-transferrin from the blood correlated directly with tumor mass. It is concluded that cellular incorporation of transferrin within the tumor cells results in a loss of circulating transferrin, which correlates with tumor mass and proliferation of tumor. This mechanism is supposed to be the cause for the hypotransferrinemia seen in patients with malignant tumors.

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Therapeutic efficacy of some extracts of hot pepper fruits (Capsicum annuum) and Sumac seeds (Rhus coriaria) on the growth of some isolated dermatophytes species

International Journal of Medical Sciences ◽

10.32441/ijms.v1i3.102 ◽

2018 ◽

Vol 1 (3) ◽

pp. 52-62

Author(s):

Sara Omran ◽

Abdulghani Alsamarai ◽

Firas Razzzaq

Keyword(s):

Antifungal Activity ◽

Fungal Infection ◽

Aqueous Extract ◽

Plant Extracts ◽

Therapeutic Efficacy ◽

Diffusion Method ◽

Quantitative Detection ◽

Hot Pepper ◽

Alcoholic Extract ◽

Dermatology Clinic

Background: Fungal infections are one of the common skin diseases with difficulty in their treatment approach. The present efficient drugs for fungal infection are limited. Aim: To determine the therapeutic efficacy of plant extracts as alternative antifungal agents. Materials and methods: 100 clinical samples [68 from female and 32 from male] were collected during the period from March to July 2017 from subjects attending Dermatology Clinic in Salah Uldean General Hospital. Fungal infection was diagnosed with using KOH wet preparation. Fungal species identified by using conventional approach. The active ingredients existing in the plant extracts were detected and analyzed through qualitative and quantitative detection technique of chemical compounds using a high performance liquid chromatographic device (HPLC). Agar diffusion method was used to determine antifungal activity of plant extracts. Results: Direct microscopic examination showed that there were (75%) positive samples, while culture shows (67%) positive samples. The isolated dermatophytes belong to Epidermophyoton, Microsporum, and Trichophyton genus. The predominant dermatophytes were T. rubrum (25%) species. The highest frequency of infection was in the age group of 11-20 years. The sensitivity of the tested fungi to the aqueous and alcoholic plant extracts varies. Alcoholic extract of the hot pepper plant was more effective as antifungal than the aqueous extract of the same plant. However, aqueous hot pepper extracts was more effective against T. mentagrophyte than that of alcoholic extract. Additionally, alcoholic Sumac extract shows higher efficacy that aqueous extract. Conclusion: Hot pepper and Sumac extracts show antifungal activity against Microsporum canis, Trichophyton rubrum and T. mentagrophyte.

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