Die Anämie bei malignen Tumorerkrankungen

1989 ◽  
Vol 28 (06) ◽  
pp. 247-254
Author(s):  
E. Aulbert
Keyword(s):  
Malignant Tumors ◽  
Gradient Centrifugation ◽  
Tumor Resection ◽  
Hemoglobin Concentration ◽  
Limiting Factor ◽  
Novikoff Hepatoma ◽  
Tumor Mass ◽  
Lysosomal Fraction ◽  
Proliferation Activity ◽  
Morris Hepatoma

The cellular uptake and lysosomal accumulation of 67Ga-labelled transferrin within tumors of different malignancy were examined using tissue fractionation and immunological techniques. As tumor models the slowly growing Morris hepatoma 5123C, the moderately growing Novikoff hepatoma and the fast and aggressive Yoshida hepatoma AH 130 were investigated. Isolation of subcellular fractions of tumor homogenates was performed by differential centrifugation and density-gradient centrifugation. The intracellular 67Gatransferrin was found to be highly concentrated within the purified lysosomes. The transferrin within the lysosomal fraction was identified by radial immunodiffusion technique using monospecific antiserum. The accumulation of 67Gatransferrin by the tumors resulted in a faster disappearance of 67Ga-transferrin from the blood. This loss of circulating 67Ga-transferrin correlated with the proliferation activity and the spread of the tumors. Since transferrin is indispensible for the utilization of iron by the heme-synthesizing red cell precursors, transferrin concentration in the blood is the limiting factor for the utilization of iron in hemoglobin synthesis. Thus, in a further series of experiments we investigated the development of anemia in tumor-bearing rats. With increasing tumor mass a progressive fall of hemoglobin concentration was found. The anemia was more severe in the faster growing Novikoff hepatoma than in the slowly growing Morris hepatoma. The most significant reduction of hemoglobin concentration was found in the very fast growing Yoshida hepatoma. After total tumor resection hemoglobin concentration and red blood cell count normalized completely within 6-8 weeks. We conclude from these data that the uptake of transferrin by the tumor cells results in a faster disappearance of transferrin from the blood. This loss of circulating transferrin correlates with tumor mass and proliferation activity and is one of the factors responsible for the anemia seen in patients with malignant tumors.

Die Anämie bei malignen Tumorerkrankungen

1989 ◽  
Vol 28 (05) ◽  
pp. 193-200 ◽  
Author(s):  
E. Aulbert
Keyword(s):  
Cellular Uptake ◽  
Tumor Tissue ◽  
Malignant Tumors ◽  
The Other ◽  
Novikoff Hepatoma ◽  
Cellular Accumulation ◽  
Tumor Mass ◽  
Proliferation Activity ◽  
Morris Hepatoma ◽  
Cellular Incorporation

Cellular uptake of 67Ga-labelled transferrin by the tumor tissue was studied in rats with tumors of different malignancy and different tumor mass using the slowly growing Morris hepatoma 5123C, the moderately growing Novikoff hepatoma and the very fast and aggressive Yoshida hepatoma AH130. The cellular accumulation of 67Ga-transferrin was found to correlate with the proliferation activity of the tumor. The 67Ga-transferrin concentration in the very fast growing Yoshida hepatoma was 4.8 times higher than the concentration in the slowly growing Morris hepatoma. The uptake of 67Ga-transferrin by the tumors resulted in a faster disappearance of circulating 67Ga-transferrin from the blood. The rate of disappearance correlated with the proliferation activity and the spread of the tumors. Using tumors of identical size the elimination of 67Ga-transferrin from the blood was much faster in the rats with Yoshida hepatoma than in those with the slowly growing Morris hepatoma. On the other hand, using tumors of different tumor size it could be demonstrated that the rate of disappearance of 67Ga-transferrin from the blood correlated directly with tumor mass. It is concluded that cellular incorporation of transferrin within the tumor cells results in a loss of circulating transferrin, which correlates with tumor mass and proliferation of tumor. This mechanism is supposed to be the cause for the hypotransferrinemia seen in patients with malignant tumors.

scholarly journals Can Strain Elastography Predict Malignancy of Soft Tissue Tumors in a Tertiary Sarcoma Center?

Diagnostics ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 148
Author(s):  
Jonathan Cohen ◽  
Iben Riishede ◽  
Jonathan Frederik Carlsen ◽  
Trine-Lise Lambine ◽  
Mikkel Seidelin Dam ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Malignant Tumors ◽  
Tumor Resection ◽  
Strain Ratio ◽  
Soft Tissue Tumors ◽  
Current Data ◽  
Strain Elastography ◽  
Valuable Adjunct ◽  
Malignant Soft Tissue Tumor ◽  
Significant Difference

This study aims to investigate the ability of ultrasound strain elastography as an adjunct to predict malignancy in soft tissue tumors suspect of sarcoma or metastasis in a tertiary reference center for sarcoma. A total of 137 patients were included prospectively. Patients were referred on the basis of clinical or radiological suspicion of malignant soft tissue tumor. All patients had previously undergone diagnostic imaging (MRI, CT or PET-CT). After recording strain elastography cine loops, ultrasound guided biopsy was performed. Three investigators, who were blinded to final diagnosis, reviewed all elastograms retrospectively. For each elastogram, a qualitative, visual 5-point score was decided in consensus and a strain ratio was calculated. Final pathology obtained from biopsy or tumor resection served as gold standard. Eighty-one tumors were benign, and 56 were malignant. t-tests showed a significant difference in mean visual score between benign and malignant tumors. There was no significant difference in mean strain ratio between the two groups. Strain elastography may be a valuable adjunct to conventional B-mode ultrasound, perhaps primarily in primary care, when considering whether to refer to a sarcoma center or to biopsy, although biopsies cannot reliably be ruled out based on the current data.

scholarly journals Extensive Growth of an Anaplastic Meningioma

2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
Hajrullah Ahmeti ◽  
Homajoun Maslehaty ◽  
Athanasios K. Petridis ◽  
Alexandros Doukas ◽  
Mehran Mahvash ◽  
...  
Keyword(s):  
Malignant Tumors ◽  
Tumor Resection ◽  
High Rate ◽  
Complete Tumor Resection ◽  
Calvarial Bone ◽  
Anaplastic Meningioma ◽  
Progressive Ataxia ◽  
Extensive Growth ◽  
Tumor Entity ◽  
Complete Tumor

We present the case of a 30-year-old male patient with an almost complete destruction of the calvarial bone through an anaplastic meningioma diagnosed in line with dizziness. Neuroimaging revealed an extensive growing, contrast enhancing lesion expanding at the supra- and infratentorial convexity, infiltrating and destroying large parts of the skull, and infiltrating the skin. Due to progressive ataxia and dysarthria with proven tumor growth in the posterior fossa in the continuing course, parts of the tumor were resected. A surgical procedure with the aim of complete tumor resection in a curative manner was not possible. Six months after the first operation, due to a new tumor progression, most extensive tumor resection was performed. Due to the aggressive and destructive growth with a high rate of recurrence and tendency of metastases, anaplastic meningiomas can be termed as malignant tumors. The extrinsic growth masks the tumor until they reach a size, which makes these tumors almost unresectable. In the best case scenarios, the five-year survival is about 50%. With the presented case, we would like to show the aggressive behavior of anaplastic meningiomas in a very illustrative way. Chemotherapy, radiotherapy, and surgery reach their limits in this tumor entity.

scholarly journals Inhibition of leucocytic lysosomal enzymes by glycosaminoglycans in vitro

1975 ◽  
Vol 152 (1) ◽  
pp. 57-64 ◽  
Author(s):  
J L Avila ◽  
J Convit
Keyword(s):  
Incubation Medium ◽  
Lysosomal Enzymes ◽  
Chondroitin Sulphate ◽  
Gradient Centrifugation ◽  
Polymorphonuclear Leucocyte ◽  
Dependent Manner ◽  
Acid Hydrolases ◽  
Lysosomal Fraction ◽  
Formation Of Complexes

1. A lysosomal fraction was separated by density-gradient centrifugation from a highly purified human polymorphonuclear leucocyte suspension. 2. Some 23 different lysosomal enzymes were assayed for activity in the presence of various concentrations of glycosaminoglycans. 3. The 21 acid hydrolases assayed were strongly inhibited to different degrees by low (0-12 mmol/l) concentrations of glycosaminoglycans in a pH-dependent manner. Thus inhibitions were stronger below pH4.5, with activity returning to control values at about pH5.0. 4. On a molar basis, the inhibitory activity for the several glycosaminoglycans studied was: heparin > chondroitin sulphate ≫ hyaluronic acid. 5. Once the glycosaminoglycan-acid hydrolase complex was formed, it was partially dissociated by slight elevations in the pH of the incubation medium, by increasing the ionic strength of the incubation medium, or by adding several cationic proteins (e.g. histone, protamine). 6. As leucocytic lysosomes contain large amounts of chondroitin sulphate, and have a strongly acid intragranular pH, we suggest that glycosaminoglycans may modify lysosomal function through the formation of complexes with lysosomal enzymes, by inhibiting the digestive activity of the acid hydrolases when the intralysosomal pH is below their pI.

Reconstruction of the skull base after tumor resection: an overview of methods

2002 ◽  
Vol 12 (5) ◽  
pp. 1-5 ◽  
Author(s):  
James K. Liu ◽  
Zahid Niazi ◽  
William T. Couldwell
Keyword(s):  
Skull Base ◽  
Malignant Tumors ◽  
Tumor Resection ◽  
Infectious Complications ◽  
Skull Base Tumors ◽  
Skull Base Reconstruction ◽  
Life Threatening ◽  
Dural Defects ◽  
Reconstructive Techniques ◽  
Do So

Successful surgical management of malignant skull base tumors depends on both tumor resection and reconstruction of the cranial base defect. The primary goals of skull base reconstruction are to repair dural defects, to prevent the development of cerebrospinal fluid fistulas, and to provide a protective barrier that isolates the intracranial contents from the nasopharynx and paranasal sinuses. Failure to do so can result in potentially life-threatening infectious complications. With modern skull base and reconstructive techniques, malignant tumors in this region, which were once deemed inoperable, can now be safely removed. The authors review the different modalities available for skull base reconstruction following tumor resection.

scholarly journals Multiple Tumor Induction after Treatment of Temporal Arteritis with Prednisone

2017 ◽  
Vol 10 (3) ◽  
pp. 1076-1084 ◽  
Author(s):  
Frank F. Piraino
Keyword(s):  
Surveillance System ◽  
Temporal Arteritis ◽  
Malignant Tumors ◽  
Lung Nodule ◽  
Temporal Analysis ◽  
Immune Markers ◽  
Tumor Mass ◽  
Multiple Tumor ◽  
Organ Systems ◽  
Autoimmune Inflammatory Disease

A 74-year-old female was diagnosed with the autoimmune inflammatory disease temporal arteritis and treated with high and low doses of prednisone over a period of 6 years. During that time, she developed cancers of the lung and colon as well as a soft tumor mass on lumbar vertebrate L3. She also experienced a series of debilitating and disabling symptoms while on prednisone treatment. A temporal analysis of the association of prednisone therapy and immune markers to the successive appearance of the malignant tumors strongly suggests that in the absence of a functioning natural immune and surveillance system by treatment with the immune knockout drug prednisone, spontaneous, multiple independent mutations occurred in several sites in the organ systems of this patient. Over a period of time, these developed into malignant cancers, including a lung nodule which became cancerous 256 days later, as well as the cancers of the colon and a soft tumor mass on lumbar vertebrate L3.

scholarly journals Assessment of prognostic factors in extracranial germ cell tumors in children

2021 ◽  
Vol 67 (6) ◽  
pp. 829-836
Author(s):  
Symbat Salieva ◽  
Riza Boranbayeva ◽  
Bakhram Zhumadullayev ◽  
Ergali Sarsekbayev ◽  
Oleg Bydanov
Keyword(s):  
Overall Survival ◽  
Prognostic Factors ◽  
Survival Rate ◽  
Germ Cell ◽  
Malignant Tumors ◽  
Survival Rates ◽  
Tumor Resection ◽  
Germ Cell Tumors ◽  
Morphological Structure ◽  
High Sensitivity

Germ cell neoplasms in the group of benign and malignant tumors heterogeneous in morphological structure, clinical features and prognosis. A special characteristic of germ cell tumors is their high sensitivity to platinum-containing chemotherapy, which allows cure of up to 80–90% patients. However 20–25% of patients with a common type have overall survival rate of less than 50%. The aim of the study is to assess the survival rate of children with extracranial germ cell tumors and to identify adverse risk factors. Methods. The study includes 116 children with extracranial germ cell tumors treated from 2013 to September 2009. Treatment consisted of tumor resection and platinum based on platinum chemotherapy. Survival rate was assessed by the Kaplan-Mayer method. Prognostic factors are determined according to IGCCCG, MaGIC, MAKEI, RODO. Results. Overall and event free survival rates were 79±5% and 76±4%, respectively. The worst overall survival had patients with extragonadal tumors, advanced stages of a disease, high initial level of AFP (≥10 000 ng/ml), non-seminoma version of state treasury bills and extra pulmonary metastases. Conclusion. Survival rate in children with extracranial germ cell tumors depends on the prognostic factors. Statistically significant predictors of the poor prognosis were extragonadal localization of a tumor and the AFP level ≥10 000 ng/ml.

scholarly journals Unclear Retroperitoneal Tumors, an Interdisciplinary Challenge – A Case Report and Review of the Literature

2021 ◽  
Author(s):  
Benno Traub ◽  
Benedikt Haggemüller ◽  
Lisa Baumann ◽  
Johannes Lemke ◽  
Doris Henne-Bruns ◽  
...  
Keyword(s):  
Case Report ◽  
Clinical Symptoms ◽  
Malignant Tumors ◽  
Tumor Resection ◽  
Vena Cava ◽  
Retroperitoneal Tumors ◽  
Common Clinical Presentation ◽  
One Year ◽  
The Right ◽  
Upper Abdomen

Abstract BackgroundUnclear retroperitoneal tumors still impose major challenges for clinicians. Tumors can originate primarily from retroperitoneal tissue or secondarily invade into the retroperitoneal space. While benign lesions also occur, malignant tumors are far more common. Clinical presentation often depends on replacement or invasion of other organs and is therefore highly variable. The heterogeneous tumor composition makes a definitive preoperative diagnosis difficult. Nevertheless, surgical resection is the gold standard for treatment but often proves challenging due to frequent involvement of large retroperitoneal vessels.Case presentationWe present a case report of a 70-year old woman diagnosed with a large, unclear retroperitoneal tumor. Initial clinical symptoms were increasing dyspnea and dysphagia in our clinic. Gastroenterologic and cardiologic workup was unremarkable. Computed Tomography (CT) revealed a large retroperitoneal mass in the right upper abdomen with severe displacement of the inferior vena cava and renal veins. Without signs of irresectability, the patient was scheduled for tumor resection. The procedure was challenging due to the vessel involvement and large blood pressure alterations during tumor mobilization. The post-op pathologic workup then revealed the rare finding of a completely resected paraganglioma. The post-surgical course was uneventful. One year after diagnosis, the patient is relapse-free.CconclusionThis case impressively shows the challenges of retroperitoneal tumors and the importance of interdisciplinary work in these cases.

scholarly journals Management of low-grade gliomas

2021 ◽  
Author(s):  
Milla Giancristofaro Dutra ◽  
Bernardo Valle Zanetti ◽  
Ana Luiza Badini Tubenchlak ◽  
Bárbara Gomes Muffato ◽  
Leonardo Moreira Dutra ◽  
...  
Keyword(s):  
Large Scale ◽  
Randomized Clinical Trials ◽  
Malignant Tumors ◽  
Tumor Resection ◽  
Progression Free Survival ◽  
Functional Results ◽  
Therapeutic Modality ◽  
Low Grade ◽  
Low Grade Gliomas ◽  
The Central Nervous System

Background: Gliomas are the most aggressive and prevalent primary malignant tumors of the central nervous system. For better mapping, they are subclassified into degrees in proportion to their malignancy. Although low-grade patients have a better prognosis, they are extremely heterogeneous. Since the high variability in the outcomes of the condition, it is essential to investigate the current therapeutic strategies available. Objective: Analyze the management of low-grade gliomas. Methods: In April 2021, a literature review was conducted on MEDLINE using the descriptors: “Glioma”, “Low Grade”; “Treatment”; as well as their variations obtained in MeSH. Controlled and randomized clinical trials carried out on humans in the last five years were included. Results: 63 articles were found and 10 of them were analyzed in this review. The research has shown that total tumor resection is the therapeutic modality that causes the greatest drop in the mortality rates. Furthermore, the greater the extraction, the greater the progression-free survival. In this way, for greater safety of large-scale surgeries, several intraoperative techniques have been developed. An example is the waking approach, which presents favorable long-term functional results and low failure rates. However, the isolated surgery is often not sufficiently curative. Therefore, it is necessary to complement radiotherapy and chemotherapy with temozolomide, associated with a 5 to 10 year survival rate when combined. Conclusions: Studies have shown that total resection of the tumor is the best way to manage low-grade gliomas, but it is often combined with temozolamide chemotherapy and radiotherapy for a better prognosis.

The Differentiation of Acid Phosphatases of Human Blood Platelets

1971 ◽  
Vol 26 (02) ◽  
pp. 353-361 ◽  
Author(s):  
H. D Kaulen ◽  
R Gross
Keyword(s):  
Rat Liver ◽  
Blood Platelets ◽  
Gradient Centrifugation ◽  
Sucrose Density Gradient ◽  
Human Platelets ◽  
Acid Phosphatases ◽  
Sucrose Density Gradient Centrifugation ◽  
Lysosomal Fraction ◽  
Hydrolysis Of ◽  
Human Blood Platelets

SummaryThe Acid Phosphatase was tested in human platelets and in the rat liver mitochondrial-lysosomal fraction with p-nitrophenylphosphate and β-glycerophosphate as substrates. In the platelets the following differences were found between the hydrolysis of these two substrates, whereas in rat liver no such differences were observed. 1. The relative rates of hydrolysis and the pH optima for both substrates are different (pH 4.6 for the β-glycerophosphatase, pH 6.0 for the p-nitrophenylphosphatase in the platelets). 2. The p-nitrophenylphosphatase of the platelets is inhibited by p-chlormercuribenzoate and N-ethylmaleimide, but not by fluoride or L + tartrate, whereas the contrary is true for the platelet β-glycerophosphatase and the rat liver activities. 3. The platelet p-nitrophenylphosphatase is rapidly inactivated by preincubation at 40-45° C for 15 min, the other phosphatases are much more heat-resistant. 4. Sucrose density gradient centrifugation of platelet homgenates showed a separation of the two platelet phosphatase activities, the p-nitrophenylphosphatase with its maximum at lower densities than the β-glycerophosphatase.It is concluded that in human platelets there are at least two different Acid Phosphatases. The β-glycerophosphatase probably represents the lysosomal (as compared to the rat liver enzyme) phosphatase whereas the p-nitrophenylphosphatase of the platelets is a different enzyme whose subcellular localization and functions are as yet unknown.

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