Frontispiece: Development of Lysosome-Targeted Fluorescent Probes for Cys by Regulating the Boron-dipyrromethene (BODIPY) Molecular Structure

A series of two-photon-absorbing pyrrolopyrrole aza boron dipyrromethenes have been prepared which can serve as fluorescent probes for subcellular imaging.

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Development of 2,6-carboxy-substituted boron dipyrromethene (BODIPY) as a novel scaffold of ratiometric fluorescent probes for live cell imaging

Chemical Communications ◽

10.1039/b917209b ◽

2009 ◽

pp. 7015 ◽

Cited By ~ 53

Author(s):

Toru Komatsu ◽

Yasuteru Urano ◽

Yuuta Fujikawa ◽

Tomonori Kobayashi ◽

Hirotatsu Kojima ◽

...

Keyword(s):

Fluorescent Probes ◽

Live Cell Imaging ◽

Cell Imaging ◽

Live Cell ◽

Boron Dipyrromethene ◽

Novel Scaffold

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Spectroscopic characterization of synthetic heteroatom-functionalized fluorescent probes for bioimaging / Sentetik heteroatom-fonksiyonelize floresans probların biyogörüntüleme uygulamaları için spektroskopik karakterizasyonu

Turkish Journal of Biochemistry ◽

10.1515/tjb-2015-0026 ◽

2015 ◽

Vol 40 (5) ◽

Author(s):

Özlem Dilek

Keyword(s):

Fluorescent Probes ◽

Emission Spectra ◽

Spectroscopic Characterization ◽

Spectroscopic Properties ◽

Absorption Bands ◽

Broad Absorption ◽

Cellular Environment ◽

Boron Dipyrromethene ◽

Biological Labeling

AbstractObjective: Fluorescent probes are essential tools for monitoring biological systems in cellular environment. Several boron dipyrromethene (BODIPY) derived fluorescent probe derivatives have been synthesized by nucleophilic substitution reaction and their spectroscopic properties have also been determined.Methods: Initial BODIPY dye framework was prepared from the reaction of pyrrole-2-carbaldehyde and benzaldehyde and then readily reacted with heteroatom-based nucleophiles at 3- and 5- position of BODIPY core. The spectroscopic properties of these new molecules have been reported.Results: Amine based nucleophiles tend to produce broad absorption bands on the spectra. Our studies indicated that heteroatom-based nucleophiles on the BODIPY core resulted in a red shift on the absorption and emission spectra.Conclusion: The synthesized fluorophores can therefore be potentially used as imaging agents for biological labeling.

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Fluorescence relaxation spectroscopy in the analysis of macromolecular structure and motion

Quarterly Reviews of Biophysics ◽

10.1017/s0033583500002122 ◽

1976 ◽

Vol 9 (1) ◽

pp. 1-19 ◽

Cited By ~ 47

Author(s):

Rudolf Rigler ◽

Måns Ehrenberg

Keyword(s):

Molecular Structure ◽

Energy Transfer ◽

Fluorescent Probes ◽

Personal Communication ◽

Macromolecular Structure ◽

Primary Sequence ◽

Experimental Conditions ◽

Structure And Motion ◽

End Distance ◽

Kinetics Of

Fluorescence spectroscopy offers particularly sensitive tools for the investigation of physical properties of macromolecules in solution. Differences in molecular structure under varying experimental conditions are often reflected in quantum yield and lifetime of natural or synthetically inserted fluorescent probes. inserted fluorescent probes. Energy transfer between fluorescent groups at known places of a primary sequence can be used to measure distances between the label sites (Eisinger, 1976) as well as to investigate their relative motion, e.g. measurements of the kinetics of variations in the end to end distance of a polymer (E. Haas & I. Steinberg, personal communication).

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