Cyclo‐Ketal Xanthene Dyes: A New Class of Near‐Infrared Fluorophores for Super‐Resolution Imaging of Live Cells

2020 ◽  
Author(s):  
Xinfu Zhang ◽  
Lingcheng Chen ◽  
Zhenlong Huang ◽  
Ni Ling ◽  
Yi Xiao
Keyword(s):  
Near Infrared ◽  
Super Resolution ◽  
Live Cells ◽  
Xanthene Dyes ◽  
New Class ◽  
Resolution Imaging

scholarly journals 3D super-resolved imaging in live cells using sub-diffractive plasmonic localization of hybrid nanopillar arrays

Nanophotonics ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 2847-2859
Author(s):  
Soojung Kim ◽  
Hyerin Song ◽  
Heesang Ahn ◽  
Seung Won Jun ◽  
Seungchul Kim ◽  
...  
Keyword(s):  
Signal To Noise Ratio ◽  
Imaging Techniques ◽  
Optical Loss ◽  
Super Resolution ◽  
Living Cells ◽  
Live Cells ◽  
Complex Biological System ◽  
Observation Volume ◽  
Resolution Imaging ◽  
Nanopillar Arrays

AbstractAnalysing dynamics of a single biomolecule using high-resolution imaging techniques has been had significant attentions to understand complex biological system. Among the many approaches, vertical nanopillar arrays in contact with the inside of cells have been reported as a one of useful imaging applications since an observation volume can be confined down to few-tens nanometre theoretically. However, the nanopillars experimentally are not able to obtain super-resolution imaging because their evanescent waves generate a high optical loss and a low signal-to-noise ratio. Also, conventional nanopillars have a limitation to yield 3D information because they do not concern field localization in z-axis. Here, we developed novel hybrid nanopillar arrays (HNPs) that consist of SiO2 nanopillars terminated with gold nanodisks, allowing extreme light localization. The electromagnetic field profiles of HNPs are obtained through simulations and imaging resolution of cell membrane and biomolecules in living cells are tested using one-photon and 3D multiphoton fluorescence microscopy, respectively. Consequently, HNPs present approximately 25 times enhanced intensity compared to controls and obtained an axial and lateral resolution of 110 and 210 nm of the intensities of fluorophores conjugated with biomolecules transported in living cells. These structures can be a great platform to analyse complex intracellular environment.

scholarly journals Targeted Molecular Imaging Using Aptamers in Cancer

2018 ◽  
Vol 11 (3) ◽  
pp. 71 ◽  
Author(s):  
Sorah Yoon ◽  
John Rossi
Keyword(s):  
Wide Spectrum ◽  
Rna Aptamers ◽  
Tissue Imaging ◽  
Live Cells ◽  
Target Cells ◽  
New Class ◽  
Non Invasive ◽  
Resolution Imaging ◽  
Tissue Specimens

Imaging is not only seeing, but also believing. For targeted imaging modalities, nucleic acid aptamers have features such as superior recognition of structural epitopes and quick uptake in target cells. This explains the emergence of an evolved new class of aptamers into a wide spectrum of imaging applications over the last decade. Genetically encoded biosensors tagged with fluorescent RNA aptamers have been developed as intracellular imaging tools to understand cellular signaling and physiology in live cells. Cancer-specific aptamers labeled with fluorescence have been used for assessment of clinical tissue specimens. Aptamers conjugated with gold nanoparticles have been employed to develop innovative mass spectrometry tissue imaging. Also, use of chemically conjugated cancer-specific aptamers as probes for non-invasive and high-resolution imaging has been transformative for in vivo imaging in multiple cancers.

scholarly journals High-dimensional super-resolution imaging reveals heterogeneity and dynamics of subcellular lipid membranes

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Karl Zhanghao ◽  
Wenhui Liu ◽  
Meiqi Li ◽  
Zihan Wu ◽  
Xiao Wang ◽  
...  
Keyword(s):  
Lipid Membranes ◽  
Developmental Stages ◽  
Membrane Separation ◽  
Optical Tomography ◽  
Super Resolution ◽  
Live Cells ◽  
Lipid Dynamics ◽  
Membrane Morphology ◽  
Intracellular Organelles ◽  
Resolution Imaging

AbstractLipid membranes are found in most intracellular organelles, and their heterogeneities play an essential role in regulating the organelles’ biochemical functionalities. Here we report a Spectrum and Polarization Optical Tomography (SPOT) technique to study the subcellular lipidomics in live cells. Simply using one dye that universally stains the lipid membranes, SPOT can simultaneously resolve the membrane morphology, polarity, and phase from the three optical-dimensions of intensity, spectrum, and polarization, respectively. These high-throughput optical properties reveal lipid heterogeneities of ten subcellular compartments, at different developmental stages, and even within the same organelle. Furthermore, we obtain real-time monitoring of the multi-organelle interactive activities of cell division and successfully reveal their sophisticated lipid dynamics during the plasma membrane separation, tunneling nanotubules formation, and mitochondrial cristae dissociation. This work suggests research frontiers in correlating single-cell super-resolution lipidomics with multiplexed imaging of organelle interactome.

scholarly journals Instant super-resolution imaging in live cells and embryos via analog image processing

2013 ◽  
Vol 10 (11) ◽  
pp. 1122-1126 ◽  
Author(s):  
Andrew G York ◽  
Panagiotis Chandris ◽  
Damian Dalle Nogare ◽  
Jeffrey Head ◽  
Peter Wawrzusin ◽  
...  
Keyword(s):  
Image Processing ◽  
Super Resolution ◽  
Live Cells ◽  
Resolution Imaging ◽  
Analog Image Processing

scholarly journals Super-resolution imaging of nuclear import of adeno-associated virus in live cells

2015 ◽  
Vol 2 ◽  
pp. 15047 ◽  
Author(s):  
Joseph M Kelich ◽  
Jiong Ma ◽  
Biao Dong ◽  
Qizhao Wang ◽  
Mario Chin ◽  
...  
Keyword(s):  
Nuclear Import ◽  
Super Resolution ◽  
Live Cells ◽  
Adeno Associated Virus ◽  
Resolution Imaging

scholarly journals Super-Resolution Imaging of the Golgi in Live Cells with a Bioorthogonal Ceramide Probe

2014 ◽  
Vol 53 (38) ◽  
pp. 10242-10246 ◽  
Author(s):  
Roman S. Erdmann ◽  
Hideo Takakura ◽  
Alexander D. Thompson ◽  
Felix Rivera-Molina ◽  
Edward S. Allgeyer ◽  
...  
Keyword(s):  
Super Resolution ◽  
Live Cells ◽  
Resolution Imaging

Revealing dynamically-organized receptor ion channel clusters in live cells by a correlated electric recording and super-resolution single-molecule imaging approach

2018 ◽  
Vol 20 (12) ◽  
pp. 8088-8098 ◽  
Author(s):  
Rajeev Yadav ◽  
H. Peter Lu
Keyword(s):  
Ion Channel ◽  
Single Molecule ◽  
Super Resolution ◽  
Live Cells ◽  
Imaging Approach ◽  
Resolution Imaging ◽  
Function Relationship ◽  
Bleaching Step ◽  
Relationship Of ◽  
Resolution Single

Correlating single-molecule fluorescence photo-bleaching step analysis and single-molecule super-resolution imaging, our findings for the clustering effect of the NMDA receptor ion channel on the live cell membranes provide a new and significant understanding of the structure–function relationship of NMDA receptors.

scholarly journals A Photoactivatable Probe for Super-Resolution Imaging of Enzymatic Activity in Live Cells

2017 ◽  
Vol 139 (37) ◽  
pp. 13200-13207 ◽  
Author(s):  
Elias A. Halabi ◽  
Zacharias Thiel ◽  
Nils Trapp ◽  
Dorothea Pinotsi ◽  
Pablo Rivera-Fuentes
Keyword(s):  
Enzymatic Activity ◽  
Super Resolution ◽  
Live Cells ◽  
Resolution Imaging

scholarly journals Fast, three-dimensional super-resolution imaging of live cells

2011 ◽  
Vol 8 (6) ◽  
pp. 499-505 ◽  
Author(s):  
Sara A Jones ◽  
Sang-Hee Shim ◽  
Jiang He ◽  
Xiaowei Zhuang
Keyword(s):  
Three Dimensional ◽  
Super Resolution ◽  
Live Cells ◽  
Resolution Imaging
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