scholarly journals 3D super-resolved imaging in live cells using sub-diffractive plasmonic localization of hybrid nanopillar arrays

Nanophotonics ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 2847-2859
Author(s):  
Soojung Kim ◽  
Hyerin Song ◽  
Heesang Ahn ◽  
Seung Won Jun ◽  
Seungchul Kim ◽  
...  
Keyword(s):  
Signal To Noise Ratio ◽  
Imaging Techniques ◽  
Optical Loss ◽  
Super Resolution ◽  
Living Cells ◽  
Live Cells ◽  
Complex Biological System ◽  
Observation Volume ◽  
Resolution Imaging ◽  
Nanopillar Arrays

AbstractAnalysing dynamics of a single biomolecule using high-resolution imaging techniques has been had significant attentions to understand complex biological system. Among the many approaches, vertical nanopillar arrays in contact with the inside of cells have been reported as a one of useful imaging applications since an observation volume can be confined down to few-tens nanometre theoretically. However, the nanopillars experimentally are not able to obtain super-resolution imaging because their evanescent waves generate a high optical loss and a low signal-to-noise ratio. Also, conventional nanopillars have a limitation to yield 3D information because they do not concern field localization in z-axis. Here, we developed novel hybrid nanopillar arrays (HNPs) that consist of SiO2 nanopillars terminated with gold nanodisks, allowing extreme light localization. The electromagnetic field profiles of HNPs are obtained through simulations and imaging resolution of cell membrane and biomolecules in living cells are tested using one-photon and 3D multiphoton fluorescence microscopy, respectively. Consequently, HNPs present approximately 25 times enhanced intensity compared to controls and obtained an axial and lateral resolution of 110 and 210 nm of the intensities of fluorophores conjugated with biomolecules transported in living cells. These structures can be a great platform to analyse complex intracellular environment.

Imaging live cells at high spatiotemporal resolution for lab-on-a-chip applications

Lab on a Chip ◽  
2016 ◽  
Vol 16 (11) ◽  
pp. 2014-2024 ◽  
Author(s):  
Lip Ket Chin ◽  
Chau-Hwang Lee ◽  
Bi-Chang Chen
Keyword(s):  
Imaging Techniques ◽  
Super Resolution ◽  
Lab On A Chip ◽  
Live Cells ◽  
Spatiotemporal Resolution ◽  
Cellular Processes ◽  
High Spatiotemporal Resolution ◽  
Microfluidic Technology ◽  
Resolution Imaging

The synergy of novel super-resolution imaging techniques and microfluidic technology provides new biological and biomedical insights into sub-cellular processes.

4-dimensional, high-resolution imaging of living cells

1992 ◽  
Vol 50 (1) ◽  
pp. 416-417
Author(s):  
Shinya Inoué
Keyword(s):  
Light Microscope ◽  
Living Cells ◽  
Live Cells ◽  
Video Tape ◽  
Active Living ◽  
High Resolution Imaging ◽  
3 Dimensional ◽  
Dynamic Architecture ◽  
Resolution Imaging ◽  
Disk Memory

This paper reports progress of our effort to rapidly capture, and display in time-lapsed mode, the 3-dimensional dynamic architecture of active living cells and developing embryos at the highest resolution of the light microscope. Our approach entails: (A) real-time video tape recording of through-focal, ultrathin optical sections of live cells at the highest resolution of the light microscope; (B) repeat of A at time-lapsed intervals; (C) once each time-lapsed interval, an image at home focus is recorded onto Optical Disk Memory Recorder (OMDR); (D) periods of interest are selected using the OMDR and video tape records; (E) selected stacks of optical sections are converted into plane projections representing different view angles (±4 degrees for stereo view, additional angles when revolving stereos are desired); (F) analysis using A - D.

De Novo-Designed Landmine Warfare Strategy Luminophore for Super-resolution Imaging Reveal ONOO- evolution in Living Cells

2021 ◽  
pp. 130151
Author(s):  
Yuanyuan Liu ◽  
Chengying Zhang ◽  
Yongchun Wei ◽  
Huimin Chen ◽  
Lingxiu Kong ◽  
...  
Keyword(s):  
De Novo ◽  
Super Resolution ◽  
Living Cells ◽  
Resolution Imaging

Super-Resolution Imaging in Fluid Mechanics Using New Illumination Approaches

2020 ◽  
Vol 52 (1) ◽  
pp. 369-393
Author(s):  
Minami Yoda
Keyword(s):  
Fluid Mechanics ◽  
Total Internal Reflection ◽  
Imaging Techniques ◽  
Super Resolution ◽  
Internal Reflection ◽  
Interfacial Flows ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Entire Volume ◽  
Resolution Imaging

Quantifying submillimeter flows using optical diagnostic techniques is often limited by a lack of spatial resolution and optical access. This review discusses two super-resolution imaging techniques, structured illumination microscopy and total internal reflection fluorescence or microscopy, which can visualize bulk and interfacial flows, respectively, at spatial resolutions below the classic diffraction limits. First, we discuss the theory and applications of structured illumination for optical sectioning, i.e., imaging a thin slice of a flow illuminated over its entire volume. Structured illumination can be used to visualize the interior of multiphase flows such as sprays by greatly reducing secondary scattering. Second, the theory underlying evanescent waves is introduced, followed by a review of how total internal reflection microscopy has been used to visualize interfacial flows over the last 15 years. Both techniques, which are starting to be used in fluid mechanics, could significantly improve quantitative imaging of microscale and macroscale flows.

scholarly journals Fluorescence imaging with tailored light

Nanophotonics ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 2111-2128 ◽  
Author(s):  
Jialei Tang ◽  
Jinhan Ren ◽  
Kyu Young Han
Keyword(s):  
Fluorescence Imaging ◽  
Imaging Techniques ◽  
Super Resolution ◽  
Optical Parameters ◽  
Propagation Angle ◽  
Resolution Imaging ◽  
Time Observation ◽  
Living Organisms ◽  
Real Time Observation

AbstractFluorescence microscopy has long been a valuable tool for biological and medical imaging. Control of optical parameters such as the amplitude, phase, polarization, and propagation angle of light gives fluorescence imaging great capabilities ranging from super-resolution imaging to long-term real-time observation of living organisms. In this review, we discuss current fluorescence imaging techniques in terms of the use of tailored or structured light for the sample illumination and fluorescence detection, providing a clear overview of their working principles and capabilities.

scholarly journals Super-Resolution Imaging by Dual Iterative Structured Illumination Microscopy

2021 ◽  
Author(s):  
Anna Loeschberger ◽  
Yauheni Novikau ◽  
Ralf Netz ◽  
Marie-Christin Spindler ◽  
Ricardo Benavente ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Brain Slices ◽  
Super Resolution ◽  
Reconstruction Algorithm ◽  
Living Cells ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Spatiotemporal Resolution ◽  
Coated Pits ◽  
Resolution Imaging

Three-dimensional (3D) multicolor super-resolution imaging in the 50-100 nm range in fixed and living cells remains challenging. We extend the resolution of structured illumination microscopy (SIM) by an improved nonlinear iterative reconstruction algorithm that enables 3D multicolor imaging with improved spatiotemporal resolution at low illumination intensities. We demonstrate the performance of dual iterative SIM (diSIM) imaging cellular structures in fixed cells including synaptonemal complexes, clathrin coated pits and the actin cytoskeleton with lateral resolutions of 60-100 nm with standard fluorophores. Furthermore, we visualize dendritic spines in 70 micrometer thick brain slices with an axial resolution < 200 nm. Finally, we image dynamics of the endoplasmatic reticulum and microtubules in living cells with up to 255 frames/s.

scholarly journals A Simple Probe for Super-Resolution Imaging of the Endoplasmic Reticulum in Living Cells

2018 ◽  
Vol 101 (11) ◽  
pp. e1800165 ◽  
Author(s):  
Elias A. Halabi ◽  
Salome Püntener ◽  
Pablo Rivera-Fuentes
Keyword(s):  
Endoplasmic Reticulum ◽  
Super Resolution ◽  
Living Cells ◽  
Resolution Imaging

scholarly journals Snapshots of archaeal DNA replication and repair in living cells using super-resolution imaging

2018 ◽  
Author(s):  
Floriane Delpech ◽  
Yoann Collien ◽  
Pierre Mahou ◽  
Emmanuel Beaurepaire ◽  
Hannu Myllykallio ◽  
...  
Keyword(s):  
Dna Replication ◽  
Super Resolution ◽  
Living Cells ◽  
Dna Replication And Repair ◽  
Resolution Imaging ◽  
Archaeal Dna Replication

Super-resolution imaging of bacterial pathogens and visualization of their secreted effectors

2020 ◽  
Author(s):  
Moirangthem Kiran Singh ◽  
Linda J Kenney
Keyword(s):  
Virulence Factors ◽  
Protein Secretion ◽  
Fluorescent Probes ◽  
Imaging Techniques ◽  
Super Resolution ◽  
Bacterial Pathogenesis ◽  
Secretion Systems ◽  
Complex Protein ◽  
Resolution Imaging ◽  
Protein Secretion Systems

ABSTRACT Recent advances in super-resolution imaging techniques, together with new fluorescent probes have enhanced our understanding of bacterial pathogenesis and their interplay within the host. In this review, we provide an overview of what these techniques have taught us about the bacterial lifestyle, the nucleoid organization, its complex protein secretion systems, as well as the secreted virulence factors.

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