ChemInform Abstract: The Fragmentation Behavior of Differently Substituted 2-Amino-1,3,4- benzotriazepines.

ChemInform ◽  
2010 ◽  
Vol 23 (20) ◽  
pp. no-no
Author(s):  
P. VAINIOTALO ◽  
P. OTTOILA ◽  
O. MORGENSTERN ◽  
P. RICHTER
Keyword(s):  
Fragmentation Behavior

scholarly journals Characterization of a novel + 70 Da modification in rhGM-CSF expressed in E. coli using chemical assays in combination with mass spectrometry

Amino Acids ◽  
2021 ◽  
Author(s):  
Magdalena Widgren Sandberg ◽  
Jakob Bunkenborg ◽  
Stine Thyssen ◽  
Martin Villadsen ◽  
Thomas Kofoed
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Peptide Mapping ◽  
Covalent Modification ◽  
Peptide Fragmentation ◽  
Tandem Mass ◽  
E Coli ◽  
Gm Csf ◽  
Fragmentation Behavior ◽  
Macrophage Colony

AbstractGranulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine and a white blood cell growth factor that has found usage as a therapeutic protein. During analysis of different fermentation batches of GM-CSF recombinantly expressed in E. coli, a covalent modification was identified on the protein by intact mass spectrometry. The modification gave a mass shift of + 70 Da and peptide mapping analysis demonstrated that it located to the protein N-terminus and lysine side chains. The chemical composition of C4H6O was found to be the best candidate by peptide fragmentation using tandem mass spectrometry. The modification likely contains a carbonyl group, since the mass of the modification increased by 2 Da by reduction with borane pyridine complex and it reacted with 2,4-dinitrophenylhydrazine. On the basis of chemical and tandem mass spectrometry fragmentation behavior, the modification could be attributed to crotonaldehyde, a reactive compound formed during lipid peroxidation. A low recorded oxygen pressure in the reactor during protein expression could be linked to the formation of this compound. This study shows the importance of maintaining full control over all reaction parameters during recombinant protein production.

Fragmentation behavior of Y 2 O 3 suspension in axially fed suspension plasma spray

2017 ◽  
Vol 309 ◽  
pp. 456-461 ◽  
Author(s):  
Jung-ki Lee ◽  
Sang-Jun Park ◽  
Yoon-Suk Oh ◽  
Seongwon Kim ◽  
Hyungsun Kim ◽  
...  
Keyword(s):  
Plasma Spray ◽  
Suspension Plasma Spray ◽  
Fragmentation Behavior

Explosively Driven Expansion and Fragmentation Behavior for Cylinders, Spheres and Rings of 304 Stainless Steel

2010 ◽  
Vol 638-642 ◽  
pp. 1035-1040 ◽  
Author(s):  
Tetsuyuki Hiroe ◽  
Kazuhito Fujiwara ◽  
Hidehiro Hata ◽  
Mitsuru Yamauchi ◽  
Kiyotaka Tsutsumi ◽  
...  
Keyword(s):  
Stainless Steel ◽  
High Speed ◽  
Fracture Behavior ◽  
Copper Wire ◽  
304 Stainless Steel ◽  
Fragmentation Model ◽  
Fragmentation Behavior ◽  
Deformation And Fracture ◽  
Test Parameters ◽  
Fine Copper

Explosive loading techniques are applied to expand tubular cylinders, spherical shells and rings of 304 stainless steel to fragmentation, and the effects of wall thicknesses, explosive driver diameters and the constant proportionality of the in-plane biaxial stretching rates are investigated on the deformation and fracture behavior of three basic structures experimentally and numerically. In the cylinder tests, the driver is a column of high explosive PETN, inserted coaxially into the bore of a cylinder and initiated by exploding a fine wire bundle at the column axis using a discharge current from a high-voltage capacitor bank. In case of the ring tests, ring specimens are placed onto a single cylinder filled with the PETN as a expansion driver, and for sphere tests, specimens filled with the PETN are also initiated by exploding a fine copper wire line with small length located at the central point. Two types of experiments are conducted for every specimen and test condition. The first type uses high speed cameras to observe the deformation and crack generation of expanding specimens showing the final maximum in-plane stretching rate of above , and the second uses soft capturing system recovering typically most fragments successfully. The fragments are measured and investigated using a fragmentation model. The effects of test parameters on the deformation and fracture behavior for three types of structures are discussed in need of modified fragmentation model for shell structural elements.

Visual Observation of Fragmentation Behavior on Molten Material Jet Surface in Coolant

2008 ◽  
Author(s):  
Yuta Uchiyama ◽  
Yutaka Abe ◽  
Akiko Fujiwara ◽  
Hideki Nariai ◽  
Eiji Matsuo ◽  
...  
Keyword(s):  
Water Surface ◽  
High Speed ◽  
Internal Flow ◽  
Core Material ◽  
Generation Process ◽  
High Speed Camera ◽  
Sodium Coolant ◽  
Molten Material ◽  
Fragmentation Behavior ◽  
Jet Breakup

For the safety design of the Fast Breeder Reactor (FBR), it is strongly required that the post accident heat removal (PAHR) is achieved after a postulated core disruptive accident (CDA). In the PAHR, it is important that the molten core material is solidified in sodium coolant which has high boiling point. Thus it is necessary to estimate the jet breakup length which is the distance that the molten core material is solidified in sodium coolant. In the previous studies (Abe et al., 2006), it is observed that the jet is broken up with fragmenting in water coolant by using simulated core material. It is pointed out that the jet breakup behavior is significantly influenced by the fragmentation behavior on the molten material jet surface in the coolant. However, the relation between the jet breakup behavior and fragmentation on the jet surface during a CDA for a FBR is not elucidated in detail yet. The objective of the present study is to elucidate the influence of the internal flow in the jet and fragmentation behavior on the jet breakup behavior. The Fluorinert™ (FC-3283) which is heavier than water and is transparent fluid is used as the simulant material of the core material. It is injected into the water as the coolant. The jet breakup behavior of the Fluorinert™ is observed by high speed camera to obtain the fragmentation behavior on the molten material jet surface in coolant in detail. To be cleared the effect of the internal flow of jet and the surrounding flow structure on the fragmentation behavior, the velocity distribution of internal flow of the jet is measured by PIV (Particle Image Velocimetry) technique with high speed camera. From the obtained images, unstable interfacial wave is confirmed at upstream of the jet surface, and the wave grows along the jet-water surface in the flow direction. The fragments are torn apart at the end of developed wave. By using PIV analysis, the velocity at the center of the jet is fast and it suddenly decreases near the jet surface. This means that the shear force acts on the jet and water surface. From the results of experiment, the correlation between the interfacial behavior of the jet and the generation process of fragments are discussed. In addition, the influence of surface instability of the jet induced by the relative velocity between Fluorinert™ and coolant water on the breakup behavior is also discussed.

scholarly journals Fragmentation Behavior Studies of Chalcones Employing Direct Analysis in Real Time (DART)

2013 ◽  
Vol 4 (2) ◽  
pp. 30-33 ◽  
Author(s):  
A.F.M. Motiur Rahman ◽  
Mohamed W. Attwa ◽  
Pervez Ahmad ◽  
Mohammad Baseeruddin ◽  
Adnan A. Kadi
Keyword(s):  
Real Time ◽  
Direct Analysis ◽  
Fragmentation Behavior

scholarly journals Identification of In Vitro Metabolites of Synthetic Phenolic Antioxidants BHT, BHA, and TBHQ by LC-HRMS/MS

2020 ◽  
Vol 21 (24) ◽  
pp. 9525
Author(s):  
Ons Ousji ◽  
Lekha Sleno
Keyword(s):  
Isotope Labeling ◽  
Butylated Hydroxytoluene ◽  
Phenolic Antioxidants ◽  
Fragmentation Behavior ◽  
Accurate Mass Measurements ◽  
Oxidative Metabolites ◽  
Biotransformation Products ◽  
Pharmaceutical Industries ◽  
Synthetic Phenolic Antioxidants

Butylated hydroxytoluene (BHT) and its analogs, butylated hydroxyanisole (BHA) and tert-butyl-hydroquinone (TBHQ), are widely used synthetic preservatives to inhibit lipid oxidation in the food, cosmetic and pharmaceutical industries. Despite their widespread use, little is known about their human exposure and related biotransformation products. The metabolism of these compounds was investigated using in vitro incubations with human and rat liver fractions. Liquid chromatography coupled to high-resolution tandem mass spectrometry was employed to detect and characterize stable and reactive species formed via oxidative metabolism, as well as phase II conjugates. Several oxidative metabolites have been detected, as well as glutathione, glucuronide, and sulfate conjugates, many of which were not previously reported. A combination of accurate mass measurements, MS/MS fragmentation behavior, and isotope-labeling studies were used to elucidate metabolite structures.

Formation of Highly Charged Quasi-Molecular Ions of a Polycationic [60]Fullerene Hexakis-Adduct and Their Fragmentation Behavior in the Gas Phase

2015 ◽  
Vol 2015 (10) ◽  
pp. 2282-2290 ◽  
Author(s):  
Jing Li ◽  
Leanne C. Nye ◽  
Lennard K. Wasserthal ◽  
Chau Vinh ◽  
Rolf W. Kirschbaum ◽  
...  
Keyword(s):  
Gas Phase ◽  
Molecular Ions ◽  
Fragmentation Behavior
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