Unidirectional growth of heavy meromyosin clusters along actin filaments revealed by real-time fluorescence microscopy

Cytoskeleton ◽  
2017 ◽  
Vol 74 (12) ◽  
pp. 482-489 ◽  
Author(s):  
Rika Hirakawa ◽  
Yusuke Nishikawa ◽  
Taro Q.P. Uyeda ◽  
Kiyotaka Tokuraku
Keyword(s):  
Fluorescence Microscopy ◽  
Real Time ◽  
Actin Filaments ◽  
Heavy Meromyosin

scholarly journals Actin-binding protein promotes the bipolar and perpendicular branching of actin filaments.

1980 ◽  
Vol 87 (3) ◽  
pp. 841-848 ◽  
Author(s):  
J H Hartwig ◽  
J Tyler ◽  
T P Stossel
Keyword(s):  
Actin Filaments ◽  
Actin Polymerization ◽  
Average Length ◽  
Binding Protein ◽  
Actin Binding ◽  
Branch Points ◽  
Heavy Meromyosin ◽  
Actin Binding Protein ◽  
Protein Dimers ◽  
Protein Molecules

Branching filaments with striking perpendicularity form when actin polymerizes in the presence of macrophage actin-binding protein. Actin-binding protein molecules are visible at the branch points. Compared with actin polymerized in the absence of actin-binding proteins, not only do the filaments branch but the average length of the actin filaments decreases from 3.2 to 0.63 micrometer. Arrowhead complexes formed by addition of heavy meromyosin molecules to the branching actin filaments point toward the branch points. Actin-binding protein also accelerates the onset of actin polymerization. All of these findings show that actin filaments assemble from nucleating sites on actin-binding protein dimers. A branching polymerization of actin filaments from a preexisting lattice of actin filaments joined by actin-binding protein molecules could generate expansion of cortical cytoplasm in amoeboid cells.

scholarly journals Nanofluidic Fluorescence Microscopy (NFM) for real-time monitoring of protein binding kinetics and affinity studies

2017 ◽  
Vol 88 ◽  
pp. 25-33 ◽  
Author(s):  
Pattamon Teerapanich ◽  
Martine Pugnière ◽  
Corinne Henriquet ◽  
Yii-Lih Lin ◽  
Chia-Fu Chou ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Protein Binding ◽  
Real Time ◽  
Binding Kinetics ◽  
Real Time Monitoring ◽  
Protein Binding Kinetics

scholarly journals Energy Metabolism and Intracellular pH Alteration in Neural Spheroids Carrying Down syndrome

Biomedicines ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 1741
Author(s):  
Alena Kashirina ◽  
Alena Gavrina ◽  
Emil Kryukov ◽  
Vadim Elagin ◽  
Yuliya Kolesova ◽  
...  
Keyword(s):  
Down Syndrome ◽  
Fluorescence Microscopy ◽  
Minimally Invasive ◽  
Real Time ◽  
Intracellular Ph ◽  
Normal Karyotype ◽  
3D Models ◽  
Brain Diseases ◽  
Fluorescence Lifetimes ◽  
Metabolic Analysis

Brain diseases including Down syndrome (DS/TS21) are known to be characterized by changes in cellular metabolism. To adequately assess such metabolic changes during pathological processes and to test drugs, methods are needed that allow monitoring of these changes in real time with minimally invasive effects. Thus, the aim of our work was to study the metabolic status and intracellular pH of spheroids carrying DS using fluorescence microscopy and FLIM. For metabolic analysis we measured the fluorescence intensities, fluorescence lifetimes and the contributions of the free and bound forms of NAD(P)H. For intracellular pH assay we measured the fluorescence intensities of SypHer-2 and BCECF. Data were processed with SPCImage and Fiji-ImageJ. We demonstrated the predominance of glycolysis in TS21 spheroids compared with normal karyotype (NK) spheroids. Assessment of the intracellular pH indicated a more alkaline intracellular pH in the TS21 spheroids compared to NK spheroids. Using fluorescence imaging, we performed a comprehensive comparative analysis of the metabolism and intracellular pH of TS21 spheroids and showed that fluorescence microscopy and FLIM make it possible to study living cells in 3D models in real time with minimally invasive effects.

Multispectral Bayesian reconstruction technique for real-time two color fluorescence microscopy

RSC Advances ◽  
2015 ◽  
Vol 5 (17) ◽  
pp. 13175-13183 ◽  
Author(s):  
Shilpa Dilipkumar ◽  
Ravi Manjithaya ◽  
Partha Pratim Mondal
Keyword(s):  
Image Reconstruction ◽  
Fluorescence Microscopy ◽  
Real Time ◽  
Spectral Imaging ◽  
Reconstruction Technique ◽  
Imaging Method ◽  
Combined Approach ◽  
Bayesian Reconstruction ◽  
Image Reconstruction Technique ◽  
Bayesian Image Reconstruction

We have developed a real-time imaging method for two-color widefield fluorescence microscopy using a combined approach that integrates multi-spectral imaging and Bayesian image reconstruction technique.

Real-Time Imaging of Immune Modulation by Cannabinoids Using Intravital Fluorescence Microscopy

2021 ◽  
Author(s):  
Juan Zhou ◽  
Kiyana Kamali ◽  
J. Daniel Lafreniere ◽  
Christian Lehmann
Keyword(s):  
Fluorescence Microscopy ◽  
Real Time ◽  
Immune Modulation ◽  
Intravital Fluorescence Microscopy ◽  
Real Time Imaging

scholarly journals Feasibility of confocal fluorescence microscopy for real-time evaluation of neoplasia in fresh human breast tissue

2013 ◽  
Vol 18 (10) ◽  
pp. 106016 ◽  
Author(s):  
Jessica L. Dobbs ◽  
Hao Ding ◽  
Ana Paula Benveniste ◽  
Henry M. Kuerer ◽  
Savitri Krishnamurthy ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Real Time ◽  
Breast Tissue ◽  
Confocal Fluorescence Microscopy ◽  
Human Breast ◽  
Human Breast Tissue ◽  
Confocal Fluorescence ◽  
Time Evaluation
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