Comprehensive Guide to Extracting and Expressing Fungal Secondary Metabolites with Aspergillus fumigatus as a Case Study

2021 ◽  
Vol 1 (12) ◽  
Author(s):  
Grant Nickles ◽  
Isabelle Ludwikoski ◽  
Jin Woo Bok ◽  
Nancy P. Keller
Keyword(s):  
Secondary Metabolites ◽  
Aspergillus Fumigatus ◽  
Fungal Secondary Metabolites

scholarly journals To Stretch the Boundary of Secondary Metabolite Production in Plant Cell-Based Bioprocessing: Anthocyanin as a Case Study

2004 ◽  
Vol 2004 (5) ◽  
pp. 264-271 ◽  
Author(s):  
Wei Zhang ◽  
Chris Franco ◽  
Chris Curtin ◽  
Simon Conn
Keyword(s):  
Secondary Metabolites ◽  
Plant Cell ◽  
Metabolic Pathways ◽  
Anthocyanin Biosynthesis ◽  
Rational Approach ◽  
Great Promise ◽  
Primary Metabolism ◽  
Global Correlation ◽  
Empirical Level

Plant cells and tissue cultures hold great promise for controlled production of a myriad of useful secondary metabolites on demand. The current yield and productivity cannot fulfill the commercial goal of a plant cell-based bioprocess for the production of most secondary metabolites. In order to stretch the boundary, recent advances, new directions and opportunities in plant cell-based bioprocessing, have been critically examined for the 10 years from 1992 to 2002. A review of the literature indicated that most of the R&D work was devoted predominantly to studies at an empirical level. A rational approach to molecular plant cell bioprocessing based on the fundamental understanding of metabolic pathways and their regulations is urgently required to stimulate further advances; however, the strategies and technical framework are still being developed. It is the aim of this review to take a step forward in framing workable strategies and technologies for molecular plant cell-based bioprocessing. Using anthocyanin biosynthesis as a case study, an integrated postgenomic approach has been proposed. This combines the functional analysis of metabolic pathways for biosynthesis of a particular metabolite from profiling of gene expression and protein expression to metabolic profiling. A global correlation not only can thus be established at the three molecular levels, but also places emphasis on the interactions between primary metabolism and secondary metabolism; between competing and/or complimentary pathways; and between biosynthetic and post-biosynthetic events.

The fungal secondary metabolites beauvericin and enniatin differentially regulate osteoclast inhibition during differentiation and bone resorption

Bone ◽  
2009 ◽  
Vol 44 ◽  
pp. S327
Author(s):  
F. Tedjiotsop-Feudjio⁎ ◽  
R. Lemmens-Gruber ◽  
O. Hoffmann
Keyword(s):  
Bone Resorption ◽  
Secondary Metabolites ◽  
Fungal Secondary Metabolites

Mycotoxins: Fungal Secondary Metabolites with Toxic Properties

Fungi ◽  
2018 ◽  
pp. 318-371
Author(s):  
P.M. Cano ◽  
O. Puel ◽  
I.P. Oswald
Keyword(s):  
Secondary Metabolites ◽  
Fungal Secondary Metabolites

scholarly journals Secondary metabolites of a marine-derived Penicillium ochrochloron

2021 ◽  
Vol 13 (3) ◽  
pp. 11020
Author(s):  
Peter M. EZE ◽  
Ying GAO ◽  
Yang LIU ◽  
Lasse Van GEELEN ◽  
Chika P. EJIKEUGWU ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Biological Properties ◽  
Industrial Applications ◽  
Biologically Active ◽  
Fungal Isolation ◽  
The North ◽  
Wide Range ◽  
Extremophilic Fungi ◽  
Fungal Secondary Metabolites

Extremophilic fungi have received considerable attention recently as new promising sources of biologically active compounds with potential pharmaceutical applications. This study investigated the secondary metabolites of a marine-derived Penicillium ochrochloron isolated from underwater sea sand collected from the North Sea in St. Peter-Ording, Germany. Standard techniques were used for fungal isolation, taxonomic identification, fermentation, extraction, and isolation of fungal secondary metabolites. Chromatographic separation and spectroscopic analyses of the fungal secondary metabolites yielded eight compounds: talumarin A (1), aspergillumarin A (2), andrastin A (3), clavatol (4), 3-acetylphenol (5), methyl 2,5-dihydro-4-hydroxy-5-oxo-3-phenyl-2-furanpropanoate (6), emodin (7) and 2-chloroemodin (8). After co-cultivation with Bacillus subtilis, the fungus was induced to express (-)-striatisporolide A (9). Compound 1 was evaluated for antibacterial activity against Staphylococcus aureus, Acinetobacter baumannii, Mycobacterium smegmatis, and M. tuberculosis, as well as cytotoxicity against THP-1 cells. The compound, however, was not cytotoxic to THP-1 cells and had no antibacterial activity against the microorganisms tested. The compounds isolated from P. ochrochloron in this study are well-known compounds with a wide range of beneficial biological properties that can be explored for pharmaceutical, agricultural, or industrial applications. This study highlights the bioprospecting potential of marine fungi and confirms co-cultivation as a useful strategy for the discovery of new natural products.

Biological Strategies To Counteract the Effects of Mycotoxins

2009 ◽  
Vol 72 (9) ◽  
pp. 2006-2016 ◽  
Author(s):  
BULENT KABAK ◽  
ALAN D. W. DOBSON
Keyword(s):  
Biological Control ◽  
Gastrointestinal Tract ◽  
Secondary Metabolites ◽  
Adverse Effects ◽  
Fungal Infection ◽  
Physical Chemical ◽  
Biological Methods ◽  
Fungal Secondary Metabolites

Mycotoxins are fungal secondary metabolites that if ingested can cause a variety of adverse effects on both humans and animals, ranging from allergic responses to death. Therefore, exposure to mycotoxins should be minimized. A variety of physical, chemical, and biological methods have been developed for decontamination and/or detoxification of mycotoxins from contaminated foods and feeds. This overview details the latest developments in the biological control of both fungal infection and mycotoxin formation and describes the detoxification of many of the most important mycotoxins by microorganisms. This review also addresses the potential for use of microorganisms as mycotoxin binders in the gastrointestinal tract of both humans and animals, thereby reducing the potential deleterious effects of exposure to these toxins.

In vitro Production of Secondary Metabolites Using Elicitor in Catharanthus roseus: A Case Study

2013 ◽  
pp. 401-419 ◽  
Author(s):  
Zahid Hameed Siddiqui ◽  
Abdul Mujib ◽  
Mahmooduzzafar ◽  
Junaid Aslam ◽  
Khalid Rehman Hakeem ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Catharanthus Roseus ◽  
In Vitro Production ◽  
Vitro Production

Fungal Secondary Metabolites as Source of Antifungal Compounds

FUNGI ◽  
2018 ◽  
pp. 89-125
Author(s):  
MISSING-VALUE Compounds
Keyword(s):  
Secondary Metabolites ◽  
Antifungal Compounds ◽  
Fungal Secondary Metabolites

Fungal Secondary Metabolites: Current Research, Commercial Aspects, and Applications

2021 ◽  
pp. 299-346
Author(s):  
Marwa Tamim A. Abdel-Wareth
Keyword(s):  
Secondary Metabolites ◽  
Fungal Secondary Metabolites
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