Comparative performance of PETase as a function of reaction conditions, substrate properties, and product accumulation

ChemSusChem ◽  
2021 ◽  
Author(s):  
Erika Erickson ◽  
Thomas J Shakespeare ◽  
Felicia Bratti ◽  
Bonnie L Buss ◽  
Rosie Graham ◽  
...  
Keyword(s):  
Reaction Conditions ◽  
Comparative Performance ◽  
Substrate Properties ◽  
Product Accumulation

scholarly journals Comparative Performance of PETase as a Function of Reaction Conditions, Substrate Properties, and Product Accumulation

ChemSusChem ◽  
2021 ◽  
Author(s):  
Erika Erickson ◽  
Thomas J. Shakespeare ◽  
Felicia Bratti ◽  
Bonnie L. Buss ◽  
Rosie Graham ◽  
...  
Keyword(s):  
Reaction Conditions ◽  
Comparative Performance ◽  
Substrate Properties ◽  
Product Accumulation

scholarly journals Comparative Performance of PETase as a Function of Reaction Conditions, Substrate Properties, and Product Accumulation

ChemSusChem ◽  
2021 ◽  
Author(s):  
Erika Erickson ◽  
Thomas J. Shakespeare ◽  
Felicia Bratti ◽  
Bonnie L. Buss ◽  
Rosie Graham ◽  
...  
Keyword(s):  
Reaction Conditions ◽  
Comparative Performance ◽  
Substrate Properties ◽  
Product Accumulation

scholarly journals Enzymatic synthesis and phosphorolysis of 4(2)-thioxo- and 6(5)-azapyrimidine nucleosides by E. coli nucleoside phosphorylases

2016 ◽  
Vol 12 ◽  
pp. 2588-2601 ◽  
Author(s):  
Vladimir A Stepchenko ◽  
Anatoly I Miroshnikov ◽  
Frank Seela ◽  
Igor A Mikhailopulo
Keyword(s):  
Purine Nucleoside Phosphorylase ◽  
Reaction Conditions ◽  
E Coli ◽  
No Formation ◽  
Structural Peculiarities ◽  
Substrate Properties ◽  
Nucleoside Phosphorylases ◽  
Derivatives Of

The trans-2-deoxyribosylation of 4-thiouracil (4SUra) and 2-thiouracil (2SUra), as well as 6-azauracil, 6-azathymine and 6-aza-2-thiothymine was studied using dG and E. coli purine nucleoside phosphorylase (PNP) for the in situ generation of 2-deoxy-α-D-ribofuranose-1-phosphate (dRib-1P) followed by its coupling with the bases catalyzed by either E. coli thymidine (TP) or uridine (UP) phosphorylases. 4SUra revealed satisfactory substrate activity for UP and, unexpectedly, complete inertness for TP; no formation of 2’-deoxy-2-thiouridine (2SUd) was observed under analogous reaction conditions in the presence of UP and TP. On the contrary, 2SU, 2SUd, 4STd and 2STd are good substrates for both UP and TP; moreover, 2SU, 4STd and 2’-deoxy-5-azacytidine (Decitabine) are substrates for PNP and the phosphorolysis of the latter is reversible. Condensation of 2SUra and 5-azacytosine with dRib-1P (Ba salt) catalyzed by the accordant UP and PNP in Tris∙HCl buffer gave 2SUd and 2’-deoxy-5-azacytidine in 27% and 15% yields, respectively. 6-Azauracil and 6-azathymine showed good substrate properties for both TP and UP, whereas only TP recognizes 2-thio-6-azathymine as a substrate. 5-Phenyl and 5-tert-butyl derivatives of 6-azauracil and its 2-thioxo derivative were tested as substrates for UP and TP, and only 5-phenyl- and 5-tert-butyl-6-azauracils displayed very low substrate activity. The role of structural peculiarities and electronic properties in the substrate recognition by E. coli nucleoside phosphorylases is discussed.

Undecagold labeling of tRNA

1991 ◽  
Vol 49 ◽  
pp. 44-45
Author(s):  
James F. Hainfeld ◽  
Kyra M. Alford ◽  
Mathias Sprinzl ◽  
Valsan Mandiyan ◽  
Santa J. Tumminia ◽  
...  
Keyword(s):  
High Resolution ◽  
Transmission Electron Micrograph ◽  
Anticodon Loop ◽  
Electron Micrograph ◽  
Reaction Conditions ◽  
Scanning Transmission Electron ◽  
Transmission Electron ◽  
Scanning Transmission

The undecagold (Au11) cluster was used to covalently label tRNA molecules at two specific ribonucleotides, one at position 75, and one at position 32 near the anticodon loop. Two different Au11 derivatives were used, one with a monomaleimide and one with a monoiodacetamide to effect efficient reactions.The first tRNA labeled was yeast tRNAphe which had a 2-thiocytidine (s2C) enzymatically introduced at position 75. This was found to react with the iodoacetamide-Aun derivative (Fig. 1) but not the maleimide-Aun (Fig. 2). Reaction conditions were 37° for 16 hours. Addition of dimethylformamide (DMF) up to 70% made no improvement in the labeling yield. A high resolution scanning transmission electron micrograph (STEM) taken using the darkfield elastically scattered electrons is shown in Fig. 3.

Comparative performance of low-ability airmen

1970 ◽  
Author(s):  
Marvin E. Grunzke ◽  
Nancy Guinn ◽  
Glenn F. stauffer
Keyword(s):  
Comparative Performance ◽  
Low Ability

Dimensionally-Controlled Hydrothermal Tm3+-doped Oxidic Nanocrystals and Their Photoluminescence Properties

2010 ◽  
Vol 1247 ◽  
Author(s):  
Rocío Calderón-Villajos ◽  
Carlos Zaldo ◽  
Concepción Cascales
Keyword(s):  
Single Crystals ◽  
Fluorescence Lifetimes ◽  
Photoluminescence Properties ◽  
Reaction Conditions ◽  
Hydrothermal Processes ◽  
Bulk Single Crystals ◽  
Template Free ◽  
Reaction Media

AbstractControlled reaction conditions in simple, template-free hydrothermal processes yield Tm-Lu2O3 and Tm-GdVO4 nanocrystals with well-defined specific morphologies and sizes. In both oxide families, nanocrystals prepared at pH 7 reaction media exhibit photoluminescence in ∼1.95 μm similar to bulk single crystals. For the lowest Tm3+ concentration (0.2 % mol) in GdVO4 measured 3H4 and 3F4 fluorescence lifetimes τ are very near to τrad.

SME’s Performance Through Comparative Performance Indicators, Measured by Business Statistics- Albania Case

2017 ◽  
Vol 9 (1) ◽  
pp. 361
Author(s):  
Godiva Rembeci
Keyword(s):  
Economic Performance ◽  
National Economy ◽  
Performance Indicators ◽  
Future Research ◽  
Productivity Measurement ◽  
Comparative Performance ◽  
Global Consensus ◽  
Research Activities ◽  
Business Statistics ◽  
National Economies

Now days there is a global consensus among all stakeholders that SMEs represent a driving force to the overall economic development, due to their significant contribution both on GDP and employment of national economies. SMEs also by numbers dominate the world business stage, although their contribution does vary among the countries. SMEs in Albania represents about 98% of the total enterprises with a contribution to national GDP for about 70%. The structure and the performance of national economy is depended very much on the economic performance and contribution of SME, that’s why most of the governments have strategic programmes which support the SME’s development. To measure SMEs’ performance and their ability to compete on national and international markets requires a lot of information in all aspects. Through this paper the author aims to measure and analyze the economic performance of SMEs operating in Albania. To achieve this objective, official data on business statistics published by national the statistical office (INSTAT) are used for two years period 2014-2015. In addition using an international framework addressed to the objective “improve the techniques for SMEs productivity measurement”, for the first time, a set of comparative performance indicators is established and in doing so, those results can be used as term of reference in future research activities in SMEs sector. From the results it came out that although the positive growth rate of GDP during the last years , the performance indicators of SMEs show a slightly negative trend, indicating indirectly the need for support, in order to empower their contribution in national economy.

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