Application of online two‐dimensional high‐performance liquid chromatography as purification procedure to determine the origin of 19‐norandrosterone in urine by gas chromatography–combustion–isotope ratio mass spectrometry

2020 ◽  
Author(s):  
Chao Wen ◽  
Tianshuo Zhu ◽  
Jingzhu Wang ◽  
Xin Liu ◽  
Shan Wang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Isotope Ratio ◽  
High Performance ◽  
Isotope Ratio Mass Spectrometry ◽  
Two Dimensional ◽  
Purification Procedure

scholarly journals In-Depth Qualitative Analysis of Lime Essential Oils Using the Off-Line Combination of Normal Phase High Performance Liquid Chromatography and Comprehensive Two-Dimensional Gas Chromatography-Quadrupole Mass Spectrometry

Foods ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 580 ◽  
Author(s):  
Mariosimone Zoccali ◽  
Barbara Giocastro ◽  
Ivana L. Bonaccorsi ◽  
Alessandra Trozzi ◽  
Peter Q. Tranchida ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Essential Oil ◽  
Qualitative Analysis ◽  
High Performance ◽  
Normal Phase ◽  
Two Dimensional ◽  
Quadrupole Mass

The present research is focused on the in-depth qualitative analysis of three types of lime essential oil (EO), viz., Key (A and B) and Persian, using the off-line combination of normal phase high performance liquid chromatography (NP-HPLC) and comprehensive two-dimensional gas chromatography–quadrupole mass spectrometry (GC × GC-QMS). The first analytical dimension (NP-HPLC) was exploited for the isolation of the hydrocarbon constituents from the oxygenated ones. Each fraction was then reduced in volume and analyzed using (cryogenic modulation) GC × GC-QMS. Peak assignment was carried out through the combined use of mass spectral database and linear retention index matching processes. The powerful four-dimensional technology enabled the separation and identification of a very high number (153) of lime essential oil volatile compounds.

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