Species diversity of freshwater shrimp in Henan Province, China, based on morphological characters and COI mitochondrial gene

Freshwater shrimp is an extremely rich species group with a long and problematic taxonomic history, attributed to its wide distribution, numerous species and similar morphology. Shrimp diversity and species identification is utmost important for fisheries management. However, identification based on morphological characteristics is difficult and complex for a non-specialist to perform. The water system of Henan Province is relatively abundant, but there are few investigations of freshwater shrimps and no description of molecular features. The aim of this study was to uncover the species diversity and phylogenetic of freshwater shrimp in Henan province by combining morphological identification and molecular species delimitation (barcoding gene: COI gene). About 1,200 freshwater shrimp samples from 46 sampling sites were collected for preliminary traditional morphological identification, 222 samples of these were been further microscopic examination and molecular delimitation. Here we used tree based method (NJ, ML) and distance based method (ABGD, bPTP) mainly to define species, detect the cryptic species and assess the validity of the barcoding in molecular. Comprehensive morphological identification and molecular delimitation results, there were 9 effective species and more than one cryptic species of freshwater shrimp in the province and moreover all of them can be identified by DNA barcoding. The results of morphological identification and molecular identification show high consistency, which proves the high efficiency in freshwater shrimp species identification of the DNA barcoding and the presence of cryptic species.

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A new cryptic arboreal species of the Cyrtodactylus brevipalmatus group (Squamata: Gekkonidae) from the uplands of western Thailand

Vertebrate Zoology ◽

10.3897/vz.71.e76069 ◽

2021 ◽

Vol 71 ◽

pp. 723-746

Author(s):

L. Lee Grismer ◽

Chatmongkon Suwannapoom ◽

Parinya Pawangkhanant ◽

Roman A. Nazarov ◽

Platon V. Yushchenko ◽

...

Keyword(s):

New Species ◽

Mitochondrial Gene ◽

Sequence Divergence ◽

Field Work ◽

Morphological Characters ◽

Sister Species ◽

Base Pairs ◽

Meristic Characters ◽

Nadh Dehydrogenase Subunit 2 ◽

A New Species

The first integrative taxonomic analysis of the Cyrtodactylus brevipalmatus group of Southeast Asia recovered two newly discovered populations from the Tenasserim Mountains in Suan Phueng District, Ratchaburi Province, Thailand as a new species described here as C. rukhadeva sp. nov. Based on 1397 base pairs of the mitochondrial gene NADH dehydrogenase subunit 2 (ND2), C. rukhadeva sp. nov. is the well-supported sister species to a clade containing three undescribed species, C. ngati, and C. cf. interdigitalis with a large uncorrected pairwise sequence divergence from other species in the brevipalmatus group ranging from 15.4–22.1%. Cyrtodactylus elok and C. brevipalmatus are recovered as poorly supported sister species and the well-supported sister lineage to the remainder of the brevipalmatus group. Cyrtodactylus rukhadeva sp. nov. is putatively diagnosable on the basis of a number of meristic characters and easily separated from the remaining species of the brevipalmatus group by a number of discrete morphological characters as well as its statistically significant wide separation in multivariate morphospace. The discovery of C. rukhadeva sp. nov. continues to underscore the unrealized herpetological diversity in the upland forests of the Tenasserim Mountains and that additional field work will undoubtedly result in the discovery of additional new species.

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A new species of Bent-toed Gecko (Squamata, Gekkonidae, Cyrtodactylus) from the Shan Plateau in eastern Myanmar (Burma)

Zootaxa ◽

10.11646/zootaxa.4624.3.1 ◽

2019 ◽

Vol 4624 (3) ◽

pp. 301-321 ◽

Cited By ~ 1

Author(s):

L. LEE GRISMER ◽

PERRY L. JR. WOOD ◽

EVAN S. H. QUAH ◽

MYINT KYAW THURA ◽

JAMIE R. OAKS ◽

...

Keyword(s):

New Species ◽

Hot Spot ◽

Mitochondrial Gene ◽

Sequence Divergence ◽

Morphological Characters ◽

Color Pattern ◽

Pairwise Sequence Divergence ◽

Taxonomic Analysis ◽

A New Species ◽

Karst Habitats

An integrative taxonomic analysis of the Cyrtodactylus linnwayensis group of the Shan Plateau recovered two new populations from isolated karst habitats near Pinlaung Town, Shan State as a new species, C. pinlaungensis sp. nov. Cyrtodactylus pinlaungensis sp. nov. is most closely related to a clade comprising C. linnwayensis and C. ywanganensis from the western edge of the Shan Plateau approximately 90 km to the northwest. Cyrtodactylus pinlaungensis sp. nov. can be distinguished from all members of the C. linnwayensis group by a number of statistically different morphological characters, discrete color pattern differences, and its heavy tuberculation. It also bears an uncorrected pairwise sequence divergence of 5.0–7.6% from all other species combined based on the mitochondrial gene ND2 and its flanking tRNAs. The discovery of this new species on the Shan Plateau continues to underscore the fact that this region is rapidly emerging as a herpetological diversity hot-spot for Myanmar.

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Molecular phylogeny of Fordini (Hemiptera: Aphididae: Pemphiginae) inferred from nuclear gene EF-1 α and mitochondrial gene COI

Bulletin of Entomological Research ◽

10.1017/s0007485307005020 ◽

2007 ◽

Vol 97 (4) ◽

pp. 379-386 ◽

Cited By ~ 17

Author(s):

H.C. Zhang ◽

G.X. Qiao

Keyword(s):

Phylogenetic Trees ◽

Host Plants ◽

Mitochondrial Gene ◽

Nuclear Gene ◽

Morphological Characters ◽

Primary Host ◽

Gene Coi ◽

The Common ◽

Different Parts ◽

Selection Of

AbstractThe tribe Fordini is a fascinating group because of its complicated life history, primary host specificity and gall-forming characteristic. Different species produce galls with different morphology on different parts of the host plants. The EF-1α-based, COI-based and combined sequences-based phylogenetic trees with three algorithms MP, ML and Bayes all strongly suggest that Fordini is a monophyletic group with two clades corresponding to two subtribes, Fordina and Melaphidina, each also monophyletic. Some important morphological characters and primary host plants of aphids were mapped onto the phylogenetic tree to analyse the division of subtribes and to uncover at which level the aphids correspond to their primary hosts, Pistacia and Rhus. Results suggest that the division of subtribes in Fordini is closely related to host selection of aphids. The evolution of gall morphology and the probable driving force behind it in this tribe were also discussed. The Fordini aphids seem to have evolved towards a better ability to manipulate their host plant, induce strong sinks and gain high reproductive success. Galls in this tribe evolved mainly along two directions to attain this goal: (i) by enlarging the gall from small bag to spherical, even big cauliflower-like, and changing the galls' location or forming two galls in their life cycle (Fordina); (ii) by moving the gall position from midrib, petiole of the leaflet, and eventually to the common petiole of the compound leaf (Melaphidina).

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Mitochondrial DNA sequence evidence supporting the recognition of a new North Atlantic Pseudostichopus species (Echinodermata: Holothuroidea)

Journal of the Marine Biological Association of the United Kingdom ◽

10.1017/s002531540401046xh ◽

2004 ◽

Vol 84 (5) ◽

pp. 1077-1084 ◽

Cited By ~ 4

Author(s):

Francisco A. Solís-Marín ◽

David S.M. Billett ◽

Joanne Preston ◽

Alex D. Rogers

Keyword(s):

Dna Sequences ◽

Mitochondrial Gene ◽

Distinct Species ◽

Molecular Study ◽

Morphological Characters ◽

Coi Gene ◽

Species Status ◽

Separate Species ◽

The Family ◽

A New Species

A new species of the synallactid sea cucumber genus Pseudostichopus is described, P. aemulatus sp. nov., based on genetic (DNA sequences of the mitochondrial gene Cytochrome Oxidase I [COI] gene) and morphological characters. A comparative molecular study with two other species of the same genus (P. villosus and P. mollis) and from a different family (Isostichopus fuscus) was carried out in order to clarify its taxonomic identity. The nucleotide distance between P. aemulatus sp. nov. and P. villosus and P. mollis is sufficient to support distinct species status. The estimated difference in the number of amino acids, coded for by a partially sequenced COI gene, within the species of the family Synallactidae ranged from 4 to 18. The phylogenetic analysis clearly supports separate species status of these sympatric morphotypes, as indicated by the morphological analysis.

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Revision of the Neotropical water scavenger beetle genus Globulosis García, 2001 (Coleoptera: Hydrophilidae: Acidocerinae)

Zootaxa ◽

10.11646/zootaxa.4232.2.10 ◽

2017 ◽

Vol 4232 (2) ◽

pp. 271 ◽

Cited By ~ 4

Author(s):

ANDREW EDWARD Z. SHORT ◽

MAURICIO GARCÍA ◽

JENNIFER C. GIRÓN

Keyword(s):

Mitochondrial Gene ◽

Genetic Data ◽

Distinct Species ◽

Morphological Characters ◽

Single Male ◽

Male Specimen ◽

Distribution Maps ◽

Lotic Habitats ◽

Gene Coi ◽

One New Species

The Neotropical water scavenger beetle genus Globulosis García, 2001 is revised. Originally described from a single male specimen from southeastern Venezuela, the genus has since been found in localities across the Guiana Shield region and beyond, including Colombia, Suriname, Guyana, and Brazil. The genus is redescribed. Morphological characters and genetic data from the mitochondrial gene COI from ten populations across its range provide support for two distinct species, corresponding loosely to geography. The type species, G. hemisphericus García, is redescribed and newly recorded from Guyana, Suriname, and Brazil (Amazonas, Para). One new species is described, Globulosis flavus sp. n. from southwestern Venezuela (Amazonas, Bolivar). The genus is associated with lotic habitats, and typically found along the margins of small to medium sized streams. High-resolution habitus and aedeagal images and distribution maps for all species are provided.

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Morphology of the first zoeal stage of Macrobrachium surinamicum Holthuis, 1948 (Malacostraca, Decapoda, Palaemonidae) hatched in the laboratory

Crustaceana ◽

10.1163/15685403-00003723 ◽

2017 ◽

Vol 90 (11-12) ◽

pp. 1337-1350

Author(s):

Rony R. R. Vieira ◽

Cleverson R. M. Santos ◽

Arthur Anker

Keyword(s):

Larval Development ◽

Ovigerous Female ◽

Morphological Characters ◽

Zoeal Stage ◽

Freshwater Shrimp ◽

Larval Stages ◽

Brazilian State ◽

Smooth Seta

The first zoeal stage of the freshwater shrimp Macrobrachium surinamicum Holthuis, 1948 is described and illustrated based on hatchlings from an ovigerous female, collected in Rio Campupema, Abaetetuba, in the northern Brazilian state of Pará, and compared with the first zoeae of several other species of the genus occurring in Brazil. Although the first zoeae of the different species of Macrobrachium are morphologically very similar, they can still be separated by a combination of morphological characters but only M. surinamicum has one smooth seta on the maxillular endopod. Although a complete larval series of M. surinamicum presently remains unknown, as pereiopods 3-5 and pleopods are absent, the outcome of this study suggests that the larval development of this species is extended and includes at least seven larval stages.

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Unrealized diversity in an urban rainforest: A new species of Lygosoma (Squamata: Scincidae) from western Sarawak, Malaysia (Borneo)

Zootaxa ◽

10.11646/zootaxa.4370.4.2 ◽

2018 ◽

Vol 4370 (4) ◽

pp. 345 ◽

Cited By ~ 3

Author(s):

BENJAMIN R. KARIN ◽

ELYSE S. FREITAS ◽

SAMUEL SHONLEBEN ◽

L. LEE GRISMER ◽

AARON M. BAUER ◽

...

Keyword(s):

New Species ◽

Species Diversity ◽

East Asian ◽

Morphological Characters ◽

Lizard Species ◽

Protein Coding ◽

East Malaysia ◽

Mitochondrial Nd1 ◽

Distinct Morphology ◽

A New Species

We collected two specimens of an undescribed species of Lygosoma from pitfall traps in an urban rainforest in Kuching and from the base of a forested hill in western Sarawak, East Malaysia. The new species is diagnosable from all south-east Asian congeners by morphological characters, and most closely resembles Lygosoma herberti from the Thai-Malay Peninsula. The new species shows substantial molecular divergence from its closest relatives in two protein-coding genes, one mitochondrial (ND1) and one nuclear (R35) that we sequenced for several south-east Asian congeners. We describe the new species on the basis of this distinct morphology and genetic divergence. It is the third species of Lygosoma known from Borneo, and highlights the continuing rise in lizard species diversity on the island. In addition, the discovery of this species from a small urban rainforest underscores the importance of preserving intact rainforest areas of any size in maintaining species diversity.

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Towards reliable identification of male Dicerura: descriptions of three new and seven poorly known species in the Palearctic region (Diptera: Cecidomyiidae, Porricondylinae)

Zootaxa ◽

10.11646/zootaxa.4422.1.5 ◽

2018 ◽

Vol 4422 (1) ◽

pp. 85

Author(s):

MATHIAS JASCHHOF ◽

VOLDEMARS SPUNGIS

Keyword(s):

New Species ◽

Species Diversity ◽

Morphological Characters ◽

Junior Synonym ◽

Character Analysis ◽

Reliable Identification ◽

Large Numbers ◽

Palearctic Region ◽

Holarctic Region ◽

Three New Species

The taxonomy of Dicerura Kieffer, 1898, a genus of Porricondylinae now comprising 32 species in the Holarctic region, is revised. Three new species are described based on morphological characters of male adults: D. jakovlevi Jaschhof & Spungis sp. nov. (from Finland), D. penttineni Jaschhof & Spungis sp. nov. (Finland), and D. yezoensis Jaschhof & Spungis sp. nov. (Japan). Dicerura padi Mamaev, 1975 syn. nov. is revealed to be a junior synonym of D. furculata Mamaev, 1968. Seven species, whose original descriptions are from a present-day perspective inadequate for the purpose of identification, are redescribed: D. barbata Mamaev, 1966; D. formosa Mamaev, 1998; D. foliicola Mamaev, 1968; D. furculata; D. iridis (Kaltenbach, 1873); D. stipator Mamaev, 1972; and D. unidentata Spungis, 1987. A key to males of Palearctic Dicerura is provided. New faunistic records are presented for D. barbata; D. complicata Spungis, 1987; D. dentata Spungis, 1979; D. formosa; D. fungicola (Mamaev, 1964); D. mixta Spungis, 1987; and D. unidentata. Male genitalic characters are shown to be useful to a certain extent for structuring the species diversity found within the genus Dicerura. The following groups of species are defined: iridis group (with nine species), dentata group (six species), fungicola group (four species), and formosa group (four species). Six of the species whose morphology is adequately known cannot be grouped within the proposed scheme, indicating the need for further character analysis and continuing search for yet undiscovered species that are assumed to exist in large numbers.

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The identity of Aster lushiensis (Asteraceae)

Phytotaxa ◽

10.11646/phytotaxa.105.1.5 ◽

2013 ◽

Vol 105 (1) ◽

pp. 29

Author(s):

ZHIXI FU ◽

CAIFEI ZHANG ◽

TIANGANG GAO

Keyword(s):

Morphological Characters ◽

Henan Province ◽

Type Specimens

Aster lushiensis (J.Q.Fu 1983: 110) Brouillet, Semple & Y.L.Chen (2011: 607) (Astereae, Asteraceae) was recently combined from the genus Gymnaster Kitamura (1937: 301) (=Miyamayomena Kitamura (1982: 409)) without a study of specimens. It is only known from the type specimens collected in Lushi County, Henan Province, China. Examination of morphological characters of types showed that it is conspecific with Chrysanthemum vestitum (Hemsley 1888: 438) Stapf (1933: t. 9330) (Anthemideae; Shih et al. 2011), and is here reduced to a synonym of the latter species.

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