Characterization of the impact of classical cell‐culture media on the response of electrochemical sensors

2021 ◽  
Author(s):  
Ayman Chmayssem ◽  
Lauriane Petit ◽  
Nicolas Verplanck ◽  
Véronique Mourier ◽  
Séverine Vignoud ◽  
...  
Keyword(s):  
Cell Culture ◽  
Electrochemical Sensors ◽  
Culture Media ◽  
Cell Culture Media ◽  
The Impact

scholarly journals Electrospun conductive gold covered polycaprolactone fibers as electrochemical sensors for O2 monitoring in cell culture media

2020 ◽  
Vol 111 ◽  
pp. 106662 ◽  
Author(s):  
Ariana Serban ◽  
Alexandru Evanghelidis ◽  
Melania Onea ◽  
Victor Diculescu ◽  
Ionut Enculescu ◽  
...  
Keyword(s):  
Cell Culture ◽  
Electrochemical Sensors ◽  
Culture Media ◽  
Cell Culture Media

Characterization of Biomimetic Mineral Coated 3D PLGA Scaffolds

2006 ◽  
Author(s):  
A. Champa Jayasuriya ◽  
Chiragkumar Shah ◽  
Vijay Goel ◽  
Nabil A. Ebraheim
Keyword(s):  
Cell Culture ◽  
Culture Media ◽  
Biological Molecules ◽  
Polymer Surfaces ◽  
Human Blood Plasma ◽  
Carbonate Apatite ◽  
X Ray Diffraction ◽  
Cell Culture Media ◽  
Salt Leaching

The bone-like carbonate apatite (BLCA) coatings can be coated biomimetically in the polymer surfaces by soaking in the simulated body fluid (SBF). This SBF contains similar ionic constituents to human blood plasma. Micro-porous 3D poly(lactic-co-glycolic acid) PLGA scaffolds were fabricated by the solvent casting/salt leaching technique using chloroform to dissolve the polymer. We accelerated the deposition of mineral on scaffolds for 1-2 days, modifying the mineralization process using surface treatments and 5x SBF. These scaffolds were analyzed by Scanning Electron Microscopy (SEM), Fourier Transform Infra-Red (FTIR) and X-ray Diffraction (XRD). The scaffolds coated with BLCA layer were placed in the 24 well plates containing 2 ml of media, such as Tris Buffered Saline-pH 7.4, cell culture media containing αMEM supplemented with 10% FBS, and 1% penicillin-streptomycin and incubated at 37°C for 21 days. The BLCA layer on surfaces of scaffold was stable even after 21 days immersed in Tris Buffered Saline and cell culture media. This study suggests that BLCA were stable for at least 3 weeks in the both media, and therefore, mineral has a potential to use as a carrier for biological molecules for localized release applications as well as bone tissue engineering applications.

Elucidating the Impact of CHO Cell Culture Media on Tryptophan Oxidation of a Monoclonal Antibody Through Gene Expression Analyses

2018 ◽  
Vol 13 (10) ◽  
pp. 1700254 ◽  
Author(s):  
Luhong He ◽  
Jairav X. Desai ◽  
Jinxin Gao ◽  
Laurie B. Hazeltine ◽  
Zhirui Lian ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Culture ◽  
Monoclonal Antibody ◽  
Culture Media ◽  
Cho Cell ◽  
Cell Culture Media ◽  
Gene Expression Analyses ◽  
Tryptophan Oxidation ◽  
Cho Cell Culture ◽  
The Impact

A Microfluidic Sensing System with a Multichannel Surface Plasmon Resonance Chip: Damage-free Characterization of Cells by Pattern Recognition

2020 ◽  
Author(s):  
Hiroka Sugai ◽  
Shunsuke Tomita ◽  
Sayaka Ishihara ◽  
Kyoko Yoshioka ◽  
Ryoji Kurita
Keyword(s):  
Cell Culture ◽  
Pattern Recognition ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Cultured Cells ◽  
Culture Media ◽  
Biological Research ◽  
Cell Culture Media

<p>The development of a versatile sensing strategy for the damage-free characterization of cultured cells is of great importance for both fundamental biological research and industrial applications. Here, we present a pattern-recognition-based cell-sensing approach using a multichannel surface plasmon resonance (SPR) chip. The chip, in which five cysteine derivatives with different structures are immobilized on Au films, is capable of generating five unique SPR sensorgrams for the cell-secreted molecules that are contained in cell culture media. An automatic statistical program was built to acquire kinetic parameters from the SPR sensorgrams and to select optimal parameters as “pattern information” for subsequent multivariate analysis. Our system rapidly (~ 10 min) provides the complex information by merely depositing a small amount of cell culture media (~ 25 µL) onto the chip, and the amount of information obtained is comparable to that furnished by a combination of conventional laborious biochemical assays. This non-invasive pattern-recognition-based cell-sensing approach could potentially be employed as a versatile tool for characterizing cells. </p>

Isolation and characterization of extracellular vesicles

2019 ◽  
Vol 1 (1) ◽  
pp. 18-26
Author(s):  
Fabia Fricke ◽  
Dominik Buschmann ◽  
Michael W. Pfaffl
Keyword(s):  
Cell Culture ◽  
Extracellular Vesicles ◽  
Culture Media ◽  
Body Fluids ◽  
Cell Culture Media ◽  
Advantages And Disadvantages ◽  
The Past ◽  
Isolation And Characterization ◽  
Basic Biology

Research into extracellular vesicles (EVs) gained significant traction in the past decade and EVs have been investigated in a wide variety of studies ranging from basic biology to diagnostic and therapeutic applications. Since EVs are secreted by most, if not all, eukaryotic and prokaryotic cells, they have been detected in body fluids as diverse as blood, urine and saliva as well as in cell culture media. In this chapter, we will provide an overview of EV isolation and characterization strategies and highlight their advantages and disadvantages.

A Microfluidic Sensing System with a Multichannel Surface Plasmon Resonance Chip: Damage-free Characterization of Cells by Pattern Recognition

2020 ◽  
Author(s):  
Hiroka Sugai ◽  
Shunsuke Tomita ◽  
Sayaka Ishihara ◽  
Kyoko Yoshioka ◽  
Ryoji Kurita
Keyword(s):  
Cell Culture ◽  
Pattern Recognition ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Cultured Cells ◽  
Culture Media ◽  
Biological Research ◽  
Cell Culture Media

<p>The development of a versatile sensing strategy for the damage-free characterization of cultured cells is of great importance for both fundamental biological research and industrial applications. Here, we present a pattern-recognition-based cell-sensing approach using a multichannel surface plasmon resonance (SPR) chip. The chip, in which five cysteine derivatives with different structures are immobilized on Au films, is capable of generating five unique SPR sensorgrams for the cell-secreted molecules that are contained in cell culture media. An automatic statistical program was built to acquire kinetic parameters from the SPR sensorgrams and to select optimal parameters as “pattern information” for subsequent multivariate analysis. Our system rapidly (~ 10 min) provides the complex information by merely depositing a small amount of cell culture media (~ 25 µL) onto the chip, and the amount of information obtained is comparable to that furnished by a combination of conventional laborious biochemical assays. This non-invasive pattern-recognition-based cell-sensing approach could potentially be employed as a versatile tool for characterizing cells. </p>

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