scholarly journals Determination of lipid content and stability in lipid nanoparticles using ultra high‐performance liquid chromatography in combination with a Corona Charged Aerosol Detector

2021 ◽  
Author(s):  
Caleb Kinsey ◽  
Tian Lu ◽  
Alyssa Deiss ◽  
Kim Vuolo ◽  
Lee Klein ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Lipid Content ◽  
High Performance ◽  
Lipid Nanoparticles ◽  
Charged Aerosol ◽  
Charged Aerosol Detector

Application of high performance liquid chromatography with a charged aerosol detector for the determination of quaternary ammonium compounds in disinfectants

2020 ◽  
pp. 18-27
Author(s):  
Daria A. Merkulova ◽  
◽  
Anastasia S. Ivanova ◽  
Anna D. Salimova ◽  
Sergey V. Andreev ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ammonium Chloride ◽  
Quaternary Ammonium ◽  
Cationic Surfactants ◽  
High Performance ◽  
Charged Aerosol ◽  
Charged Aerosol Detector ◽  
Ammonium Compounds

Cationic surfactants based disinfectants have been widely used in practice for over 20 years. Despite the significant advances in analytical chemistry during this period, the determination of mixtures of several quaternary ammonium compounds in one disinfectant still causes certain difficulties. This work describes the separation in the combined presence of alkyldimethylbenzylammonium chloride, alkyldimethyl (ethylbenzyl) ammonium chloride, and dimethyldioctylammonium chloride in disinfectants using high performance liquid chromatography with a charged aerosol detector. The method was tested on model solutions and real disinfectants. The linearity range for alkyldimethylbenzylammonium chloride was from 0.03 to 0.17 %, for alkyldimethyl (ethylbenzyl) ammonium chloride – from 0.03 to 0.15 %, and from 0.01 to 0.11 % for dimethyldioctylammonium chloride. The calculated relative error of the method did not exceed 9 %. Thus, the proposed method can be used to analyze preparative forms of disinfectants containing these and other cationic surfactants.

scholarly journals High-Performance Liquid Chromatography Determination of Free Sugars and Mannitol in Mushrooms Using Corona Charged Aerosol Detection

2020 ◽  
Author(s):  
Aneta Sławińska ◽  
Ewa Jabłońska-Ryś ◽  
Anna Stachniuk
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Limit Of Detection ◽  
Free Sugars ◽  
Charged Aerosol ◽  
Charged Aerosol Detector ◽  
Refractive Index Detector ◽  
First Time

Abstract Refractive index detector is usually used in the analysis of sugars in mushrooms, which is characterized by poor sensitivity, reproducibility, and susceptibility to interference from co-eluting sample components. In the current study, identification and determination of free sugars in mushroom samples by high-performance liquid chromatography coupled to corona charged aerosol detector (HPLC-CAD) were presented for the first time. The best chromatographic separation was performed on a Shodex Asahipak NH2P-50 4E 5 μm and mobile phase composed of 75% acetonitrile and 25% water with flow rate was 1 mL/min. The developed method offers good linearity in concentration range 0.001–0.01 or 0.01–0.2 mg/mL for tested compounds with R2 > 0.99. Limit of detection (LOD) for analytes was in the range of 7.1–120.2 ng on column. HPLC-CAD method showed very good reproducibility (RSD < 5.1%). Fructose, mannitol, and glucose were detected in all examined mushroom samples. For white Agaricus bisporus, mannitol was the most abundant sugar (7.575 mg/g dw), whereas trehalose for Pleurotus ostreatus (3.426 mg/g dw). The developed method was successfully applied for quantification of free sugars and mannitol in mushrooms. The optimized method proved to be sensitive, reproducible, and accurate.

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