Effects of External Mass Transfer and Product Inhibition on a Simulated Immobilized Enzyme-Catalyzed Reactor for Lactose Hydrolysis

2004 ◽  
Vol 4 (4) ◽  
pp. 326-340 ◽  
Author(s):  
A. E. AL-Muftah ◽  
I. M. Abu-Reesh
2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Shakeel Ahmed Ansari ◽  
Rukhsana Satar ◽  
Syed Kashif Zaidi ◽  
Abrar Ahmad

The present study demonstrates the immobilization of Aspergillus oryzae β-galactosidase on cellulose acetate-polymethylmethacrylate (CA-PMMA) membrane and its application in hydrolyzing lactose in dairy industries. The effect of physical and chemical denaturants like pH, temperature, product inhibition by galactose, storage stability, and reuse number of the enzyme immobilized on CA-PMMA membrane has been investigated. Lactose was hydrolyzed from milk and whey in batch reactors at 50°C by free and immobilized β-galactosidase (IβG). Optimum pH for the free and immobilized enzyme was found to be the same, that is, 4.5. However, IβG retained greater fractions of catalytic activity at lower and higher pH ranges. The temperature optimum for the immobilized enzyme was increased by 10°C. Moreover, Michaelis-Menten constant was increased for IβG as compared to the native one while maximum reaction rate was reduced for the immobilized enzyme. The preserved activity of free and immobilized enzyme was found to be 45% and 83%, respectively, after five weeks of storage at 4°C. Reusability of IβG was observed to be 86% even after fifth repeated use, thereby signifying its application in lactose hydrolysis (as shown in lab-scale batch reactors) in various dairy products including milk and whey.


1982 ◽  
Vol 47 (11) ◽  
pp. 3013-3018
Author(s):  
František Kaštánek ◽  
Jindřich Zahradník ◽  
Germanico Ocampo

Calculation procedure is suggested for flow intensity of substrate toward reaction interface of immobilized enzyme at simultaneous effect of enzymatic reaction and internal diffusion. The approximate model is presented in an analytical form for the basic type of Michaelis-Menten kinetics and for the case of inhibition in excess of substrate.


Author(s):  
А.В. АКУЛИЧ ◽  
Л.А. ГОСТИНЩИКОВА

Исследованы закономерности внешнего массообмена при сушке ягодного сырья для различных способов энергоподвода. Установлено, что ягоды при сушке подвержены значительной объемной (72–81%) и линейной (35–43%) усадке, которую необходимо учитывать при расчете коэффициентов массоотдачи. Объекты исследования – ягоды черники, красной и черной смородины. Проведены экспериментальные исследования по сушке ягодного сырья при конвективном и конвективном с ИК-излучением способах энергоподвода. Исследования проведены при скорости сушильного агента хса1,2 м/с и температуре tса 70°С. Получены критериальные уравнения внешнего массоообмена при сушке ягод для периода постоянной скорости, учитывающие объемную усадку. Определено, что при сушке с конвективным энергоподводом объемная усадка при одном и том же значении критерия Рейнольдса оказывает на 12–20% большее влияние на интенсивность массоообмена для красной смородины по сравнению с черникой и черной смородиной. При сушке с конвективным энергоподводом, ИК-излучением и предварительным прокалыванием объемная усадка наиболее существенно влияет на массообмен для черной смородины, протекающий в 1,5–1,8 раза интенсивнее по сравнению с черникой и красной смородиной. Полученные критериальные уравнения могут быть использованы при расчете и проектировании установок для сушки ягодного сырья. The laws of external mass transfer during drying of berry raw materials for various methods of energy supply are investigated. It is established that the berries during drying are subject to significant volumetric (72–81%) and surface (35–43%) shrinkage, which must be taken into account when calculating the mass transfer coefficients. The objects of the study were various berries: blueberries, red and black currants. Experimental studies have been carried out on the drying of selected berries both in the case of convective and convective methods with IR radiation. Investigations were carried out at a drying agent velocity хса1,2 m/s and its temperature tca70°С. The criterion equations of external mass transfer during drying of berries for a period of constant speed, taking into account the volume shrinkage, are obtained. It was determined that during drying with convective energy supply, volume shrinkage with the same value of the Reynolds criterion on 12–20% has a greater effect on the mass transfer rate for red currants. When drying with convective energy supply, IR radiation and preliminary piercing, the volume shrinkage most significantly affects the mass transfer for black currants, which is 1,5–1,8 times more intense than blueberries and red currants. The obtained criterial equations can be used in the calculation and design of berries dryers.


2006 ◽  
Vol 71 (8-9) ◽  
pp. 957-967 ◽  
Author(s):  
Ljiljana Markovska ◽  
Vera Meshko ◽  
Mirko Marinkovski

The isotherms and kinetics of zinc adsorption from aqueous solution onto granular activated carbon (GAC) and natural zeolite were studied using an agitated batch adsorber. The maximum adsorption capacities of GAC and natural zeolite towards zinc(II) from Langmuir adsorption isotherms were determined using experimental adsorption equilibrium data. The homogeneous solid diffusion model (HSD-model) combined with external mass transfer resistance was applied to fit the experimental kinetic data. The kinetics simulation study was performed using a computer program based on the proposed mathematical model and developed using gPROMS. As the two-mass transfer resistance approach was applied, two model parameters were fitted during the simulation study. External mass transfer and solid phase diffusion coefficients were obtained to predict the kinetic curves for varying initial Zn(II) concentration at constant agitation speed and constant adsorbent mass. For any particular Zn(II) - adsorbent system, k f was constant, except for the lowest initial concentration, while D s was found to increase with increasing initial Zn(II) concentration.


1992 ◽  
Vol 9 (2) ◽  
pp. 109-120 ◽  
Author(s):  
Mohammad S. El-Geundi

The adsorption of basic dyestuffs (Basic Blue 69 and Basic Red 22) onto natural clay has been studied using a series of batch adsorption runs. The pore diffusion model (PDM) has been developed based on external mass transfer and pore diffusion to predict the performance of a batch adsorber. A computer program has been developed to generate theoretical Sherwood number-time curves and these results were adjusted to experimental Sherwood number-time curves by means of a ‘best fit’ approach. The variables of initial dye concentration and natural clay mass have been successfully correlated using a single external mass-transfer coefficient, Ks, and a single effective pore diffusion coefficient, Deff. The Ks values are 3.3 × 10−5 and 2.6 × 10−5 m/s for Basic Blue 69 and Basic Red 22, respectively. The Deff values are 7.3 × 10−10 and 9.6 × 10−10 m2/s for Basic Blue 69 and Basic Red 22, respectively.


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