Cordyceps militaris extract induces apoptosis and pyroptosis via caspase‐3/PARP/GSDME pathways in A549 cell line

Valproic acid inhibit non-small-cell lung cancer (A549 cell line) metastasis via inhibition of CD44v6 and Nm23H1

10.31219/osf.io/j6tcq ◽

2019 ◽

Cited By ~ 1

Author(s):

Sorush Niknamian

Keyword(s):

Lung Cancer ◽

Valproic Acid ◽

Cell Line ◽

Histone Deacetylase ◽

A549 Cell ◽

Histone Deacetylase Inhibitors ◽

Caspase 3 ◽

Cancer Cell Line ◽

Lung Cancer Cell Line ◽

A549 Cell Line

Currently, epigenetic changes are considered to be one of the most important factors in the incidence of cancer and its metastasis to various tissues. It has been shown that CD44v6 and Nm23-H1 genes play a crucial role in the metastasis of various cancers. However, no study has been done on the effect of epigenetic factors on the expression of CD44v6 and Nm23-H1 genes in the lung cancer cell line, A549.Therefore, the present study investigated the role of a histone deacetylase inhibitor, valproic acid, on the expression of CD44v6 and Nm23-H1 genes and proteins in the A549 cell line. In this study, the A549 cell line was treated with valproic acid at concentrations of 2.2 mM, 4.5 mM, and 9 mM. We then investigated the expression of CD44v6 and Nm23-H1 genes and proteins, as well as the expression of MMP-2 and MMP-9 genes and caspase-3 activity. The results showed that valproic acid significantly decreased the expression of CD44v6 gene and protein and MMP-2 and MMP-9 genes, but increased the expression of Nm23H1 gene and protein and the activity of caspase-3 (p˂ 0.05). Our results showed that histone deacetylases affected the expression of CD44v6, Nm23-H1, MMP-2, and MMP-9, which are involved in metastasis. Therefore, the use of histone deacetylase inhibitors can be effective in the suppression of metastases and the treatment of lung cancer.

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Anticancer Properties of Fluorinated Aminophenylhydrazines on A549 Lung Carcinoma Cell Line

Iranian Journal of Public Health ◽

10.18502/ijph.v50i3.5596 ◽

2021 ◽

Author(s):

Zafer Hasan Ali SAK ◽

Faruk SÜZERGÖZ ◽

Veli Tarık KASUMOV ◽

Ali Osman GÜROL

Keyword(s):

Lung Cancer ◽

Cell Line ◽

Schiff Bases ◽

A549 Cell ◽

Caspase 3 ◽

A549 Cell Line ◽

Cytotoxic Effects ◽

Antiproliferative Effects ◽

Anticancer Properties ◽

A549 Lung

Background: Non-small cell lung cancer (NSCLC) is responsible for up to 85% of deaths associated with lung cancer. Chemotherapy is still an important treatment method on the treatment of inoperable cases. In this study, the anticancer properties of a series of Schiff bases were tested on the A549 cell line representing NSCLC. Methods: Fluorinated Schiff bases (compounds 1-6) were synthesized based on 2-amino phenylhydrazines and benzaldehydes containing fluorine were used. The cytotoxic effects of the compounds on the A549 cell line were determined by colorimetric MTT assay and the antiproliferative effects of the compounds on the A549 cell line by the CFSE method. To demonstrate the development of apoptosis, cleaved caspase-3 expression in cells was tested using the immunofluorescence method. Morphological changes indicating apoptosis in cells were determined by histopathological staining methods (H & E, giemza, PAP). Results: The strongest cytotoxic effect on A549 lung cancer cells was obtained with compound 6 (IC50: 0.64 μM) containing 5 fluorine atoms. The strongest antiproliferative effect on A549 cells was achieved with compound 5 (PI: 4.95) carrying 2 fluorine atoms. Apoptosis induction was effective in cell death. In addition to cleaved caspase-3 expression, chromatin condensation, marginalization, and apoptotic bodies were observed in the cells. Conclusion: Some of the compounds tested showed high cytotoxic and antiproliferative effects, indicating that these compounds could be potential chemotherapeutic agent candidates for lung cancer. The result of immunofluorescence and immunohistochemical analysis showing that the cytotoxic effects have been induced by apoptosis is an important advantage.

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