NCOA2is a candidate target gene of 8q gain associated with clinically aggressive prostate cancer

Introduction. miR-199a has been reported as an oncogene of various cancers. However, the biological function and regulatory mechanism of miR-199a in keratinocytes of cholesteatoma are still unclear. Methods. Detection by qRT-PCR was conducted on miR-199a’s expression in both thirty pairs of cholesteatoma tissues and normal skins. For characterizing the function of miR-199a, this research adopted transwell assay, wound healing assay, and CCK8 assays. Under the support of qRT-PCR, efforts were made to investigate the relative expression of candidate target genes. Moreover, the evaluation of the targeting relationship between miR-199a and the candidate target gene was conducted with the dual-luciferase reporter assay. Results. The upregulation of miR-199a was found in cholesteatoma tissues, which facilitated the proliferation, migration, and invasion of HaCaT cells, while its downregulation caused opposite results. Conclusions. The findings of the present research offer more insights into the molecular mechanism of cholesteatoma progression.

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Expression pattern of MMAB: Candidate target gene of HOX

Neuroscience Research ◽

10.1016/s0168-0102(98)82196-2 ◽

1998 ◽

Vol 31 ◽

pp. S278

Author(s):

Ryuichi Yamada ◽

Kosei Takeuchi ◽

Yuko Muroyama ◽

Masato Hagiwara ◽

Daihachiro Tomotsune ◽

...

Keyword(s):

Expression Pattern ◽

Target Gene ◽

Candidate Target ◽

Candidate Target Gene

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Myeloid cell leukemia-1 (Mc1-1) is a candidate target gene of hypoxia-inducible factor-1 (HIF-1) in the testis

Reproductive Biology and Endocrinology ◽

10.1186/1477-7827-10-104 ◽

2012 ◽

Vol 10 (1) ◽

pp. 104 ◽

Cited By ~ 8

Author(s):

Michael A Palladino ◽

Anoop Shah ◽

Rebecca Tyson ◽

Jaclyn Horvath ◽

Christine Dugan ◽

...

Keyword(s):

Target Gene ◽

Hypoxia Inducible Factor ◽

Myeloid Cell ◽

Cell Leukemia ◽

Hypoxia Inducible Factor 1 ◽

Candidate Target ◽

Candidate Target Gene

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MicroRNA-5572 Is a Novel MicroRNA-Regulating SLC30A3 in Sporadic Amyotrophic Lateral Sclerosis

International Journal of Molecular Sciences ◽

10.3390/ijms21124482 ◽

2020 ◽

Vol 21 (12) ◽

pp. 4482

Author(s):

Hisaka Kurita ◽

Saori Yabe ◽

Tomoyuki Ueda ◽

Masatoshi Inden ◽

Akiyoshi Kakita ◽

...

Keyword(s):

Amyotrophic Lateral Sclerosis ◽

Motor Neurons ◽

Target Gene ◽

Target Genes ◽

Sporadic Amyotrophic Lateral Sclerosis ◽

Novel Mirna ◽

Sporadic Als ◽

Candidate Target ◽

Candidate Target Gene ◽

Lateral Sclerosis

Amyotrophic lateral sclerosis (ALS) is a progressive degenerative disease caused by the loss of motor neurons. Although the pathogenesis of sporadic ALS (sALS) remains unclear, it has recently been suggested that disorders of microRNA (miRNA) may be involved in neurodegenerative conditions. The purpose of this study was to investigate miRNA levels in sALS and the target genes of miRNA. Microarray and real-time RT-PCR analyses revealed significantly-decreased levels of miR-139-5p and significantly increased levels of miR-5572 in the spinal cords of sALS patients compared with those in controls. We then focused on miR-5572, which has not been reported in ALS, and determined its target gene. By using TargetScan, we predicted SLC30A3 as the candidate target gene of miR-5572. In a previous study, we found decreased SLC30A3 levels in the spinal cords of sALS patients. We revealed that SLC30A3 was regulated by miR-5572. Taken together, these results demonstrate that the level of novel miRNA miR-5572 is increased in sALS and that SLC30A3 is one of the target genes regulated by miR-5572.

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Obesity-associated exosomal miRNAs modulate glucose and lipid metabolism in mice

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1808855115 ◽

2018 ◽

Vol 115 (48) ◽

pp. 12158-12163 ◽

Cited By ~ 62

Author(s):

Carlos Castaño ◽

Susana Kalko ◽

Anna Novials ◽

Marcelina Párrizas

Keyword(s):

Glucose Intolerance ◽

Central Obesity ◽

Target Gene ◽

Obese Mice ◽

Glucose And Lipid Metabolism ◽

Exosomal Mirnas ◽

Candidate Target ◽

Candidate Target Gene ◽

Pparα Agonist

Obesity is frequently associated with metabolic disease. Here, we show that obesity changes the miRNA profile of plasma exosomes in mice, including increases in miR-122, miR-192, miR-27a-3p, and miR-27b-3p. Importantly, treatment of lean mice with exosomes isolated from obese mice induces glucose intolerance and insulin resistance. Moreover, administration of control exosomes transfected with obesity-associated miRNA mimics strongly induces glucose intolerance in lean mice and results in central obesity and hepatic steatosis. Expression of the candidate target gene Ppara is decreased in white adipose tissue but not in the liver of mimic-treated (MIMIC) mice, and this is accompanied by increased circulating free fatty acids and hypertriglyceridemia. Treatment with a specific siRNA targeting Ppara transfected into exosomes recapitulates the phenotype induced by obesity-associated miRNAs. Importantly, simultaneously reducing free fatty acid plasma levels in MIMIC mice with either the lipolysis inhibitor acipimox or the PPARα agonist fenofibrate partially protects against these metabolic alterations. Overall, our data highlight the central role of obesity-associated exosomal miRNAs in the etiopathogeny of glucose intolerance and dyslipidemia.

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