scholarly journals Novel method of cell line establishment utilizing fluorescence-activated cell sorting resulting in 6 new head and neck squamous cell carcinoma lines

Head & Neck ◽  
2015 ◽  
Vol 38 (S1) ◽  
pp. E459-E467 ◽  
Author(s):  
John Henry Owen ◽  
Martin P. Graham ◽  
Steven B. Chinn ◽  
Owen F. Darr ◽  
Douglas B. Chepeha ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Cell Line ◽  
Squamous Cell ◽  
Cell Sorting ◽  
Neck Squamous Cell Carcinoma ◽  
Fluorescence Activated Cell Sorting ◽  
Cell Line Establishment ◽  
Novel Method

scholarly journals No direct effects of erythropoietin beta on a head and neck squamous cell carcinoma cell line which is growth stimulatedin vivo

2009 ◽  
Vol 48 (7) ◽  
pp. 1062-1069 ◽  
Author(s):  
Yutaka Sasaki ◽  
Elisabeth Kjellén ◽  
Hiroyuki Mineta ◽  
Johan Wennerberg ◽  
Lars Ekblad
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Cell Line ◽  
Squamous Cell ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Neck Squamous Cell Carcinoma ◽  
Direct Effects ◽  
Squamous Cell Carcinoma Cell

An experimental study of the combined effect of radiotherapy and chemotherapy on head and neck squamous cell carcinoma cell line

2001 ◽  
Vol 28 ◽  
pp. S83-S86
Author(s):  
Masahiko Takizawa ◽  
Satoshi Fukuda ◽  
Masaki Yokohama ◽  
Yukiko Miyatake ◽  
Yukio Inuyama
Keyword(s):  
Experimental Study ◽  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Cell Line ◽  
Squamous Cell ◽  
Carcinoma Cell ◽  
Combined Effect ◽  
Neck Squamous Cell Carcinoma ◽  
Squamous Cell Carcinoma Cell

Quantification of a panel of prognostic markers gives a novel method for predicting radiosensitivity in head and neck squamous cell carcinoma cell lines

2007 ◽  
Vol 25 (18_suppl) ◽  
pp. 6082-6082
Author(s):  
K. B. Roberg ◽  
L. Farnebo ◽  
L. Norberg-Spaak
Keyword(s):  
Squamous Cell Carcinoma ◽  
Prognostic Factors ◽  
Protein Expression ◽  
Western Blot ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Cell Lines ◽  
Squamous Cell ◽  
Neck Squamous Cell Carcinoma ◽  
Novel Method

6082 Background: The present study was undertaken to evaluate the possibility of using the expression of a panel of proteins involved in apoptosis and/or growth control as predictive markers for radiotherapy response. Methods: Nine head and neck squamous cell carcinoma (HNSCC) cell lines were selected to represent different parts of the spectrum of in vitro radiosensitivity. These cell lines have an intrinsic radiosensitivity (IR) between 1.4 and 2.6 measured with a 96 well plate clonogenic assay. Results: Fourteen proteins e.g. Bcl-2, Bcl-XL, Bax, Bad, Bak, Puma, survivin, COX-2, Hsp70, MDM2, p53, EGFR, cyklin D1 and SMAD4 were investigated in above mentioned cell lines and in normal oral keratinocytes (NOK). Each protein was quantified using Western blot analyse and the relative densiometric value from each blot was adjusted for actin and standardized to NOK. The correlation between each protein and IR was analyzed but none significant correlation was found. When combine different proteins, EGFR, Survivin, Bcl-2, Bak, Hsp70 and Smad4 together show the highest significant correlation to the IR. As Western blot is a semi quantitative method we decided that a division into groups depending on the level of protein expression would give a more realistic description of the prognostic value of the detected changes The protein expression was classified into four groups (0–4 point; no, small, middle or large changes compared to NOK) and each p53 mutation got one point. We named this grouping Number of Negative Points (NNP). The NNP for all 15 factors together show a significant correlations to IR, R=0.826 and P= 0.006. A multivariate statistical calculation was carried out to select the combination of factors that had the strongest correlation to IR and the best combination was EGFR, survivin and p53 mutations, R=0.935 and P<0.001. Conclusions: Our results show that it is only possible to predict the IR of tumor cells if combinations of prognostic factors can be analysed. Taken together, we here present a novel method that gives the possibility to combine different prognostic factors and which also enhances the chance to predict the radiosensitivity of HNSCC cells. No significant financial relationships to disclose.

scholarly journals Radioimmunotherapy of experimental head and neck squamous cell carcinoma (HNSCC) with E6-specific antibody using a novel HPV-16 positive HNSCC cell line

2011 ◽  
Vol 3 (1) ◽  
pp. 9 ◽  
Author(s):  
Matthew Harris ◽  
Xing Wang ◽  
Zewei Jiang ◽  
Gary L Goldberg ◽  
Arturo Casadevall ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Cell Line ◽  
Squamous Cell ◽  
Specific Antibody ◽  
Neck Squamous Cell Carcinoma ◽  
Hpv 16 ◽  
Hnscc Cell ◽  
Hnscc Cell Line

(Cyto)genomic and epigenetic characterization of BICR 10 cell line and three new established primary human head and neck squamous cell carcinoma cultures

2019 ◽  
Vol 41 (10) ◽  
pp. 1207-1221
Author(s):  
Ilda P. Ribeiro ◽  
Joana M. Rodrigues ◽  
Alexandra Mascarenhas ◽  
Vanessa Marques ◽  
Francisco Caramelo ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Cell Line ◽  
Squamous Cell ◽  
Human Head ◽  
Neck Squamous Cell Carcinoma
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