Hypoxia increases normal prostate epithelial cell resistance to receptor-mediated apoptosisviaAKT activation

Prostate cancer (PCa) is the most common non-cutaneous cancer and second leading cause of cancer-related death for men in the United States. The nonprotein coding gene locus plasmacytoma variant translocation 1 (PVT1) is located at 8q24 and is dysregulated in different cancers. PVT1 gives rise to several alternatively spliced transcripts and microRNAs. There are at least twelve exons of PVT1, which make separate transcripts, and likely have different functions. Here, we demonstrate that PVT1 exon 9 is significantly overexpressed in PCa tissues in comparison to normal prostate tissues. Both transient and stable overexpression of PVT1 exon 9 significantly induced greater prostate epithelial cell migration, as well as increased proliferation and corresponding proliferating cell nuclear antigen (PCNA) expression. Notably, implantation into mice of a non-tumorigenic prostate epithelial cell line stably overexpressing PVT1 exon 9 resulted in the formation of malignant tumors. Furthermore, PVT1 exon 9 overexpression significantly induced castration resistance. Consequently, PVT1 exon 9 expression is important for PCa initiation and progression, and holds promise as a therapeutic target in PCa.

Download Full-text

Novel Human Prostate Epithelial Cell Cultures

Advances in Experimental Medicine and Biology - Human Cell Transformation ◽

10.1007/978-3-030-22254-3_7 ◽

2019 ◽

pp. 91-99

Author(s):

Johng S. Rhim

Keyword(s):

Epithelial Cell ◽

Cell Cultures ◽

Human Prostate ◽

Prostate Epithelial Cell ◽

Epithelial Cell Cultures ◽

Human Prostate Epithelial Cell

Download Full-text

The Importance of LDL and Cholesterol Metabolism for Prostate Epithelial Cell Growth

PLoS ONE ◽

10.1371/journal.pone.0039445 ◽

2012 ◽

Vol 7 (6) ◽

pp. e39445 ◽

Cited By ~ 39

Author(s):

Teemu J. Murtola ◽

Heimo Syvälä ◽

Pasi Pennanen ◽

Merja Bläuer ◽

Tiina Solakivi ◽

...

Keyword(s):

Cell Growth ◽

Epithelial Cell ◽

Cholesterol Metabolism ◽

Prostate Epithelial Cell

Download Full-text

Androgen induces differentiation of a human papillomavirus 16 E6/E7 immortalized prostate epithelial cell line

Journal of Endocrinology ◽

10.1677/joe.0.1700287 ◽

2001 ◽

Vol 170 (1) ◽

pp. 287-296 ◽

Cited By ~ 41

Author(s):

MT Ling ◽

KW Chan ◽

CK Choo

Keyword(s):

Human Papillomavirus ◽

Epithelial Cell ◽

Cell Line ◽

Specific Antigen ◽

Telomerase Activity ◽

Epithelial Cell Line ◽

Prostate Cell ◽

Human Papillomavirus 16 ◽

Prostate Epithelial Cell ◽

Prostate Epithelial Cells

Androgen signaling is crucial for the growth and development, as well as for tumorigenesis of the prostate. However, many of the prostate epithelial cell lines developed previously, either normal or tumorigenic, do not express androgen receptor (AR) or respond to androgen. In order to advance our understanding on how androgen signaling regulates the growth and the differentiation status, and affects tumorigenicity of the epithelial cell, we performed experiments on HPr-1, a prostate cell line recently immortalized from normal human prostate epithelial cells. In the present study, AR was stably transfected into HPr-1 cells by replication-defective retrovirus. Treatment of HPr-1AR cells with androgen resulted in cell differentiation and growth retardation accompanied with up-regulation of cytokeratins K8 and K18, prostate specific antigen, p21 and p27, and down-regulation of c-myc, bcl-2 and telomerase activity. Our results suggest that androgen promotes the process of differentiation in a human papillomavirus 16 E6/E7 immortalized prostate epithelial cell line which may reflect the normal effects of androgen on prostate cells.

Download Full-text