Induction of plasma membrane alterations in AKR-2B mouse embryo fibroblasts by endogenous growth factors from malignant human cells

1984 ◽  
Vol 33 (1) ◽  
pp. 139-146 ◽  
Author(s):  
Michael E. Marks ◽  
Michael G. Brattain
Keyword(s):  
Plasma Membrane ◽  
Growth Factors ◽  
Endogenous Growth ◽  
Mouse Embryo ◽  
Human Cells ◽  
Mouse Embryo Fibroblasts ◽  
Embryo Fibroblasts

Effects of glycated low-density lipoprotein on cell viability, proliferation, and growth factors of mouse embryo fibroblasts

2013 ◽  
Vol 91 (1) ◽  
pp. 64-70
Author(s):  
Ruozhi Zhao ◽  
Xueping Xie ◽  
Garry X. Shen
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Cell Viability ◽  
Mouse Embryo ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density ◽  
Mouse Embryo Fibroblasts ◽  
Embryo Fibroblasts ◽  
Fibroblast Growth Factor Basic

The predominant cause of death in diabetic patients is atherosclerotic coronary artery disease (CAD). Major gross cellular changes in the vascular wall of persons with CAD include endothelial injury and foam cell formation, as well as smooth muscle cell and fibroblast proliferation. This study examined the effects of glycated low density lipoprotein (glyLDL), a biochemical marker of diabetes, on cell viability, proliferation, and the expression of multiple growth factors in mouse embryo fibroblasts (MEF). The results demonstrated that exposure to ≥150 μg/mL of glyLDL for 24 h or 100 μg/mL of glyLDL for ≥48 h either significantly reduced cell viability or increased DNA fragmentation in MEF. GlyLDL treatment (25–100 μg/mL for up to 12 h) significantly increased the abundance of proliferating cell nuclear antigen (PCNA) and achieved a peak after 4 h exposure to glyLDL. Abundances of fibroblast growth factor-basic (FGF), transforming growth factor-β (TGF), and platelet-derived growth factor-A (PDGF) in MEF reached maximal levels after 2 h exposure to 50 μg/mL of glyLDL. The maximal increase of vascular endothelial growth factor (VEGF) was detected in MEF after 4 h of exposure to 50 μg/mL of glyLDL. Inhibitors for FGF (AZD4547), VEGF, or PDGF receptors (Axitinib), but not that for TGF receptor (LY364947), significantly decreased the abundance of (PCNA) in endothelial cells. The findings suggest that early exposure to a low dosage of glyLDL transiently increases the proliferation of MEF through the upregulation of FGF, VEGF, and (or) PDGF, and prolonged exposure to high concentrations of glyLDL reduced cell viability, which possibly accelerates atherogenesis under diabetic condition.

scholarly journals Rapamycin-independent IGF2 expression in Tsc2-null mouse embryo fibroblasts and human lymphangioleiomyomatosis cells

PLoS ONE ◽  
2018 ◽  
Vol 13 (5) ◽  
pp. e0197105 ◽  
Author(s):  
Blanca E. Himes ◽  
Kseniya Obraztsova ◽  
Lurong Lian ◽  
Maya Shumyatcher ◽  
Ryan Rue ◽  
...  
Keyword(s):  
Mouse Embryo ◽  
Null Mouse ◽  
Mouse Embryo Fibroblasts ◽  
Embryo Fibroblasts ◽  
Igf2 Expression

Effects of lipopolysaccharide and RU 41740 on prostaglandin production and proliferation of mouse embryo fibroblasts in culture

1984 ◽  
Vol 15 (1-2) ◽  
pp. 66-68
Author(s):  
P. Weinling ◽  
S. Durant ◽  
P. Smets ◽  
R. Zalisz ◽  
D. Duval ◽  
...  
Keyword(s):  
Mouse Embryo ◽  
Prostaglandin Production ◽  
Mouse Embryo Fibroblasts ◽  
Embryo Fibroblasts

SWAP-70 contributes to spontaneous transformation of mouse embryo fibroblasts

2016 ◽  
Vol 345 (2) ◽  
pp. 150-157 ◽  
Author(s):  
Yu-Tzu Chang ◽  
Chung-Li Shu ◽  
Jing-Yang Lai ◽  
Ching-Yu Lin ◽  
Chih-Pin Chuu ◽  
...  
Keyword(s):  
Mouse Embryo ◽  
Spontaneous Transformation ◽  
Mouse Embryo Fibroblasts ◽  
Embryo Fibroblasts

scholarly journals Differential ability of a T-antigen transport-defective mutant of simian virus 40 to transform primary and established rodent cells.

1985 ◽  
Vol 5 (5) ◽  
pp. 1043-1050 ◽  
Author(s):  
R E Lanford ◽  
C Wong ◽  
J S Butel
Keyword(s):  
Cell Lines ◽  
Mouse Embryo ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Wild Type ◽  
Mouse Embryo Fibroblasts ◽  
Established Cell Lines ◽  
Established Cell ◽  
Embryo Fibroblasts

The transforming potential and oncogenicity of a simian virus 40 (SV40) mutant affecting T-antigen (T-ag), SV40(cT)-3, was examined in an effort to dissect T-ag functions in transformation. SV40(cT)-3 has a point mutation at nucleotide 4434 that abolishes the transport of T-ag to the nucleus but does not affect its association with the cell surface. Transfection-transformation assays were performed with primary cells and established cell lines of mouse and rat origin. The efficiency of transformation for established cell lines by SV40(cT)-3 was comparable to that of wild-type SV40, indicating that transformation of established cell lines can occur in the absence of detectable amounts of nuclear T-ag. Transformation of primary mouse embryo fibroblasts by SV40(cT)-3 was markedly influenced by culture conditions; the relative transforming frequency was dramatically reduced in assays involving focus formation in low serum concentrations or anchorage-independent growth. Immunofluorescence tests revealed that the transformed mouse embryo fibroblasts partially transport the mutant cT-ag to the cell nucleus. Transformed cell lines induced by SV40(cT)-3 did not differ in growth properties from wild-type transformants. SV40(cT)-3 was completely defective for the transformation of primary baby rat kidney cells, a primary cell type unable to transport the mutant T-ag to the nucleus. The intracellular localization of cellular protein p53 was found to mimic T-ag distribution in all the transformants analyzed. The mutant virus was weakly oncogenic in vivo: the induction of tumors in newborn hamsters by SV40(cT)-3 was reduced in incidence and delayed in appearance in comparison to wild-type SV40. These observations suggest that cellular transformation is regulated by both nuclear and surface-associated forms of SV40 T-ag.

Sign in / Sign up

Export Citation Format

Share Document