Disparity analysis and prognostic value of pretreatment whole blood Epstein‐Barr virus DNA load and EBER status in lymphomas: A retrospective multicenter study in Huaihai Lymphoma Working Group

Abstract Elevated Epstein-Barr virus (EBV) DNA load is common in lymphomas. However, it remains unclear whether the disparity in viral load and its prognostic value in lymphomas are correlated with EBER status. In this retrospective multicenter study, we collected the data of pretreatment whole blood EBV DNA (pre-EBV DNA) and EBER status and evaluated their disparity and prognostic values in lymphomas. A total of 454 lymphoma patients from December 2014 to August 2020 were retrospectively retrieved. Mann-Whitney U test, Kruskal-Wallis test, and Bonferroni's adjustment were used to explore the disparity of EBV DNA and EBER status in lymphomas. Time-dependent receiver operating characteristic analysis and MaxStat analysis were used to determine optimal cut-off points of pre-EBV DNA load. Univariable and multivariable Cox proportional hazards models were established for the estimation of prognostic factors. The positive rate of EBV-DNA in NKTL patients was higher than that in DLBCL, FL, and HL patients, and the median positive pre-EBV copy number of NKTL was also higher than that of FL and DLBCL. EBV-DNA could clearly distinguish the prognosis of DLBCL, NKTL, HL and PTCL, and the integration of EBER status and EBV DNA could differentiate the prognosis of HL patients. Multivariable results revealed that pre-EBV DNA load had an effect on the prognosis of NKTL, FL and DLBCL. The status of pre-EBV DNA and EBER were disparate. Whole blood pre-EBV DNA predicted the prognosis of lymphomas, and the integration of EBV and EBER status could differentiate the prognosis of HL Disclosures No relevant conflicts of interest to declare.

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Tu1491 THE RISK FOR LYMPH NODE METASTASIS IN EPSTEIN-BARR VIRUS-ASSOCIATED GASTRIC CARCINOMA WITH SUBMUCOSAL INVASION IS QUITE LOW: A RETROSPECTIVE MULTICENTER STUDY

Gastrointestinal Endoscopy ◽

10.1016/j.gie.2020.03.3663 ◽

2020 ◽

Vol 91 (6) ◽

pp. AB588

Author(s):

Yosuke Tsuji ◽

Tetsuo Ushiku ◽

Hiroharu Yamashita ◽

Yasuyuki Seto ◽

Masashi Fukayama ◽

...

Keyword(s):

Lymph Node ◽

Lymph Node Metastasis ◽

Gastric Carcinoma ◽

Multicenter Study ◽

Epstein Barr Virus ◽

Submucosal Invasion ◽

Barr Virus ◽

Node Metastasis ◽

Epstein Barr ◽

Retrospective Multicenter Study

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Simultaneous detection of Epstein - Barr virus and Cytomegalovirus DNA in Mini-pooling of Whole blood Samples from Iraqi blood donors by Multiplex Real-Time PCR Assay

10.36295/asro.2020.231441 ◽

2020 ◽

Vol 23 (14) ◽

Author(s):

Ghinwa S. Majid ◽

Ahmed S. Abdulamir ◽

Abbas M. Ahmed ◽

Iman M. Aufi ◽

Orooba I. Abdullah

Keyword(s):

Real Time Pcr ◽

Whole Blood ◽

Epstein Barr Virus ◽

Blood Donors ◽

Simultaneous Detection ◽

Pcr Assay ◽

Blood Samples ◽

Barr Virus ◽

Whole Blood Samples ◽

Epstein Barr

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Comparison of QIAsymphony Automated and QIAamp Manual DNA Extraction Systems for Measuring Epstein-Barr Virus DNA Load in Whole Blood Using Real-Time PCR

Journal of Molecular Diagnostics ◽

10.1016/j.jmoldx.2011.07.006 ◽

2011 ◽

Vol 13 (6) ◽

pp. 695-700 ◽

Cited By ~ 10

Author(s):

Stella Laus ◽

Lawrence A. Kingsley ◽

Michael Green ◽

Robert M. Wadowsky

Keyword(s):

Real Time ◽

Dna Extraction ◽

Real Time Pcr ◽

Whole Blood ◽

Epstein Barr Virus ◽

Barr Virus ◽

Epstein Barr

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Prognostic value of gross tumor regression and plasma Epstein Barr Virus DNA levels at the end of intensity-modulated radiation therapy in patients with nasopharyngeal carcinoma

Radiotherapy and Oncology ◽

10.1016/j.radonc.2018.10.010 ◽

2019 ◽

Vol 132 ◽

pp. 223-229 ◽

Cited By ~ 9

Author(s):

Shao-Bo Liang ◽

Ning Zhang ◽

Dan-Ming Chen ◽

Xing-Li Yang ◽

Bin-Hong Chen ◽

...

Keyword(s):

Radiation Therapy ◽

Nasopharyngeal Carcinoma ◽

Prognostic Value ◽

Epstein Barr Virus ◽

Tumor Regression ◽

Intensity Modulated Radiation Therapy ◽

Barr Virus ◽

Intensity Modulated ◽

Epstein Barr

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Pretreatment Epstein-Barr virus DNA in whole blood is a prognostic marker in peripheral T-cell lymphoma

Oncotarget ◽

10.18632/oncotarget.21251 ◽

2017 ◽

Vol 8 (54) ◽

pp. 92312-92323 ◽

Cited By ~ 3

Author(s):

Yu Ri Kim ◽

Soo-Jeong Kim ◽

June-Won Cheong ◽

Haerim Chung ◽

Ji Eun Jang ◽

...

Keyword(s):

T Cell ◽

Prognostic Marker ◽

Whole Blood ◽

Epstein Barr Virus ◽

Cell Lymphoma ◽

T Cell Lymphoma ◽

Peripheral T Cell Lymphoma ◽

Barr Virus ◽

Epstein Barr

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Epstein-Barr Virus Load in Whole Blood Correlates With HIV Surrogate Markers and Lymphoma: A French National Cross-Sectional Study

JAIDS Journal of Acquired Immune Deficiency Syndromes ◽

10.1097/qai.0b013e31819a22ec ◽

2009 ◽

Vol 50 (4) ◽

pp. 427-429 ◽

Cited By ~ 8

Author(s):

Corinne Amiel ◽

Jérôme LeGoff ◽

François Xavier Lescure ◽

Marianne Coste-Burel ◽

Claire Deback ◽

...

Keyword(s):

Whole Blood ◽

Epstein Barr Virus ◽

Cross Sectional Study ◽

Surrogate Markers ◽

Sectional Study ◽

Cross Sectional ◽

Virus Load ◽

Barr Virus ◽

Epstein Barr

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Frequent monitoring of Epstein-Barr virus DNA load in unfractionated whole blood is essential for early detection of posttransplant lymphoproliferative disease in high-risk patients

Blood ◽

10.1182/blood.v97.5.1165 ◽

2001 ◽

Vol 97 (5) ◽

pp. 1165-1171 ◽

Cited By ~ 222

Author(s):

Servi J. C. Stevens ◽

Erik A. M. Verschuuren ◽

Inge Pronk ◽

Wim van der Bij ◽

Martin C. Harmsen ◽

...

Keyword(s):

Whole Blood ◽

Epstein Barr Virus ◽

Lymphoproliferative Disease ◽

Serum Samples ◽

Barr Virus ◽

Load Monitoring ◽

Risk Patients ◽

Posttransplant Lymphoproliferative Disease ◽

Ebv Dna ◽

Epstein Barr

Posttransplant lymphoproliferative disease (PTLD) is a frequent and severe Epstein-Barr virus (EBV)–associated complication in transplantation recipients that is caused by iatrogenic suppression of T-cell function. The diagnostic value of weekly EBV DNA load monitoring was investigated in prospectively collected unfractionated whole blood and serum samples of lung transplantation (LTx) recipients with and without PTLD. In PTLD patients, 78% of tested whole blood samples were above the cut-off value of quantitative competitive polymerase chain reaction (Q-PCR) (greater than 2000 EBV DNA copies per mL blood), with the majority of patients having high viral loads before and at PTLD diagnosis. Especially in a primary EBV-infected patient and in patients with conversion of immunosuppressive treatment, rapid increases in peripheral blood EBV DNA load diagnosed and predicted PTLD. In non-PTLD transplantation recipients, only 3.4% of the whole blood samples was above the cut-off value (P < .0001) despite heavy immune suppression and cytomegalovirus (CMV)-related disease. These findings illustrate the clinical importance of frequent EBV DNA load monitoring in LTx recipients. The increased EBV DNA loads in PTLD patients were restricted to the cellular blood compartment, as parallel serum samples were all below cut-off value, which indicates absence of lytic viral replication. EBV+ cells in PTLD patients have a very short doubling time, which can be as low as 56 hours, thereby creating the need for high screening frequency in high-risk patients. Furthermore, it is shown that EBV and CMV can reactivate independently in LTx recipients and that EBV DNA load monitoring may be useful in discriminating PTLD from rejection.

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