Cultured human keratinocytes for optical transmission measurement

The thermal conductivity of amorphous silicon (a-Si) thin films is determined by using the non-intrusive, in-situ optical transmission measurement. The thermal conductivity of a-Si is a key parameter in understanding the mechanism of the recrystallization of polysilicon (p-Si) during the laser annealing process to fabricate the thin film transistors with uniform characteristics which are used as switches in the active matrix liquid crystal displays. Since it is well known that the physical properties are dependent on the process parameters of the thin film deposition process, the thermal conductivity should be measured. The temperature dependence of the film complex refractive index is determined by spectroscopic ellipsometry. A nanosecond KrF excimer laser at the wavelength of 248 nm is used to raise the temperature of the thin films without melting of the thin film. In-situ transmission signal is obtained during the heating process. The acquired transmission signal is fitted with predictions obtained by coupling conductive heat transfer with multi-layer thin film optics in the optical transmission measurement.

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Evaluation and analysis of Ag photodoping in chalcogenide film by optical transmission measurement

Journal of Non-Crystalline Solids ◽

10.1016/s0022-3093(87)80681-6 ◽

1987 ◽

Vol 95-96 ◽

pp. 777-784 ◽

Cited By ~ 10

Author(s):

Takeshi Kawaguchi ◽

Shigeo Maruno ◽

Kanji Masui

Keyword(s):

Optical Transmission ◽

Transmission Measurement ◽

Chalcogenide Film ◽

Optical Transmission Measurement

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Picosecond nonlinear optical transmission measurement in SiO2/TiO2 one-dimensional photonic crystals

10.1117/12.691562 ◽

2006 ◽

Author(s):

Min Jung Kim ◽

Jisoo Hwang ◽

Seung Mook Lee ◽

Bum Ku Rhee ◽

Ilsin An ◽

...

Keyword(s):

Photonic Crystals ◽

Nonlinear Optical ◽

Optical Transmission ◽

Transmission Measurement ◽

One Dimensional ◽

Optical Transmission Measurement

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Formation of New Semiconducting Ge-Si-Fe Alloy on Si(100) and its Optical Properties

MRS Proceedings ◽

10.1557/proc-421-287 ◽

1996 ◽

Vol 421 ◽

Author(s):

H. Chen ◽

P. Han ◽

X.D. Huang ◽

L.Q. Hu ◽

Y. Shi ◽

...

Keyword(s):

Band Gap ◽

Optical Transmission ◽

High Vacuum ◽

Transmission Measurement ◽

Alloy Thin Film ◽

Direct Band Gap ◽

Reactive Deposition ◽

Direct Band ◽

Evaporation Technique ◽

Vacuum Evaporation Technique

AbstractWe report a new semiconducting Ge-Si-Fe alloy thin film grown on Si(100) by reactive deposition epitaxy(RDE) using high vacuum evaporation technique. AES and XRD results show that the new alloy can be regarded as a distorted β-FeSi2 with the Ge participation. The direct band gap of the Ge-Si-Fe alloy was determined to be 0.83eV by optical transmission measurement, which means a red shift of band gap compared with that of β-FeSi2 (Eg=0.87eV).

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Transient Optical Transmission Measurement in Excimer-Laser Irradiation of Amorphous Silicon Films

Journal of Heat Transfer ◽

10.1115/1.2910645 ◽

1993 ◽

Vol 115 (1) ◽

pp. 178-183 ◽

Cited By ~ 16

Author(s):

H. K. Park ◽

X. Xu ◽

C. P. Grigoropoulos ◽

N. Do ◽

L. Klees ◽

...

Keyword(s):

Thin Film ◽

Film Thickness ◽

Amorphous Silicon ◽

Laser Irradiation ◽

Excimer Laser ◽

Optical Transmission ◽

Elevated Temperatures ◽

Transient Temperature ◽

Transmission Measurement ◽

Excimer Laser Irradiation

The transient temperature field development during heating of an amorphous silicon (a-Si) film, deposited on a fused quartz substrate, by pulsed excimer laser irradiation is studied. Static reflectivity and transmissivity measurements are used to obtain the thin film optical properties at elevated temperatures. Experimental in-situ, transient, optical transmission data are compared with heat transfer modeling results. The variation with temperature of the material complex refractive index across the thin film thickness is taken into account. The effects of the film thickness and thermal diffusivity, as well as of the laser pulse shape, are discussed.

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COMPARISON OF CdS AND ZnS THIN FILMS PREPARED BY CHEMICAL BATH DEPOSITION

Surface Review and Letters ◽

10.1142/s0218625x09012871 ◽

2009 ◽

Vol 16 (03) ◽

pp. 469-474 ◽

Cited By ~ 15

Author(s):

QI LIU ◽

GUOBING MAO

Keyword(s):

Thin Films ◽

Chemical Bath Deposition ◽

Optical Transmission ◽

Deposition Process ◽

Xrd Analysis ◽

Transmission Measurement ◽

Rate Determining Step ◽

Cds Thin Film ◽

Cds Film ◽

Cds Films

In this paper, we reported a comparative study of process and structural characterization of cadmium sulfide ( CdS ) and zinc sulfide ( ZnS ) thin films by chemical bath deposition (CBD) technique. Their deposition process is similar which clearly shows three different growth phases, a none-film one, a quasi-linear one, and a saturation one. And the growth rate of CBD- CdS is higher than that of CBD- ZnS . Through calculating the activation energy of reaction, we can conclude that the rate determining step of CBD- CdS in the deposition process is a chemical one, in contrast with the CBD- ZnS in which the determining step can be a diffusion step. The XRD analysis of the as-deposited films shows that the CBD- CdS films present cubic and hexagonal mixed structure and CBD- ZnS films are in cubic ZnS structure. The deposition time significantly influenced the crystal structure of CdS thin film. Transmission measurement shows that when the wavelength is below 500 nm, the optical transmission of CBD- ZnS film is higher than that of CBD- CdS film. Optical transmission of both CBD- ZnS and CBD- CdS films is about 90% when the wavelength is over 600 nm. The band gap (Eg) value of CBD- CdS is about 2.41 eV and that of CBD- ZnS is about 3.51 eV.

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Kalinin: A new epithelial basement membrane adhesion molecule

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100085198 ◽

1991 ◽

Vol 49 ◽

pp. 178-179

Author(s):

Douglas R. Keene ◽

Gregory P. Lunstrum ◽

Patricia Rousselle ◽

Robert E. Burgeson

Keyword(s):

Basement Membrane ◽

Culture Media ◽

Polyacrylamide Gel Electrophoresis ◽

Human Keratinocytes ◽

Positive Staining ◽

Human Amnion ◽

Epithelial Basement Membrane ◽

Type Vii Collagen ◽

Keratinocyte Cell ◽

Epithelial Basement

A mouse monoclonal antibody produced from collagenase digests of human amnion was used by LM and TEM to study the distribution and ultrastructural features of an antigen present in epithelial tissues and in cultured human keratinocytes, and by immunoaffinity chromatography to partially purify the antigen from keratinocyte cell culture media.By immunofluorescence microscopy, the antigen displays a tissue distribution similar to type VII collagen; positive staining of the epithelial basement membrane is seen in skin, oral mucosa, trachea, esophagus, cornea, amnion and lung. Images from rotary shadowed preparations isolated by affinity chromatography demonstrate a population of rod-like molecules 107 nm in length, having pronounced globular domains at each end. Polyacrylamide gel electrophoresis suggests that the size of this molecule is approximately 440kDa, and that it is composed of three nonidentical chains disulfide bonded together.

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Alterations of ultrastructure and elemental composition in cultured human epidermal keratinocytes after treatment with nitrofurazone and silver sulfadiazine

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100127803 ◽

1987 ◽

Vol 45 ◽

pp. 676-677

Author(s):

A. R. Crooker ◽

M. C. Myers ◽

T. L. Beard ◽

E. S. Graham

Keyword(s):

Electron Microscopy ◽

Elemental Composition ◽

Pyrolytic Carbon ◽

Human Keratinocytes ◽

Silver Sulfadiazine ◽

Epidermal Keratinocytes ◽

Human Epidermal Keratinocytes ◽

Culture Systems ◽

Cytotoxicity Endpoints

Cell culture systems have become increasingly popular as a means of screening toxic agents and studying toxic mechanisms of drugs and other chemicals at the cellular and subcellular levels. These in vitro tests can be conducted rapidly in a broad range of relevant mammalian culture systems; a variety of biological and biochemical cytotoxicity endpoints can be examined. The following study utilized human keratinocytes to evaluate the relative cytotoxicities of nitrofurazone (NF) and silver sulfadiazine (SS), the active ingredients of FURACIN(R) Topical Cream and SILVADENE(R) Cream, respectively. These compounds are anti-infectives used in the treatment of burn patients. Cell ultrastructure and elemental composition were utilized as cytotoxicity endpoints.Normal Human Epidermal Keratinocytes (HK) were prepared from the EpiPackTM culture system (Clonetics Corporation, Boulder, CO). For scanning electron microscopy (SEM) and transmission electron microscopy (TEM), cells were seeded on sterile 35 mm Falcon plastic dishes; for elemental microanalysis, cells were plated on polished pyrolytic carbon discs (E. Fullam, Latham, NY) placed in the culture dishes.

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