Effect of the chemical composition ofRicinus communis polyurethane on rat bone marrow cell attachment, proliferation, and differentiation

2002 ◽  
Vol 64A (1) ◽  
pp. 171-176 ◽  
Author(s):  
M�rcio M. Beloti ◽  
Karen R. N. Hiraki ◽  
Valdemar M. R. Barros ◽  
Adalberto L. Rosa
Keyword(s):  
Bone Marrow ◽  
Chemical Composition ◽  
Bone Marrow Cell ◽  
Cell Attachment ◽  
Marrow Cell ◽  
Proliferation And Differentiation ◽  
Rat Bone

scholarly journals Proof of Concept for an Automated Image Analysis Method to Quantify Rat Bone Marrow Hematopoietic Lineages on H&E Sections

2018 ◽  
Vol 46 (3) ◽  
pp. 336-347 ◽  
Author(s):  
Cleopatra Kozlowski ◽  
Aaron Fullerton ◽  
Gary Cain ◽  
Paula Katavolos ◽  
Joseph Bravo ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Image Analysis ◽  
Cell Density ◽  
Bone Marrow Cell ◽  
Marrow Cell ◽  
Automated Image Analysis ◽  
Computer Algorithm ◽  
Analysis Method ◽  
Image Analysis Method ◽  
Rat Bone

The bone marrow is an important site for assessment of the hematopoietic toxicity of new drug candidates. Here, we extended our previous work, where we developed a computer algorithm to automatically quantitate overall bone marrow cell density by analyzing digitized images of standard hematoxylin and eosin (H&E) slides of rat bone marrow and further evaluated the capability to quantify myeloid: erythroid + lymphoid (M:EL) ratio and megakaryocyte cell density. We tested the algorithm in a toxicity study, where rats were dosed with two molecules known to affect bone marrow composition, monomethyl auristatin E, and a Bcl-xL inhibitor. The image analysis method detected significant changes in M:EL and megakaryocyte number that were either not found or semiquantitatively described by manual microscopic observation of the same slides. The image analysis results were consistent with other more established but time-consuming methods that measure changes in bone marrow cell composition: smear cytology, flow cytometry, and microscopic assessment. Our work demonstrates the feasibility of a rapid and more quantitative assessment of changes in bone marrow cell lineage composition using a computer algorithm compared to microscopic examination of H&E-stained bone marrow sections.

Rat Bone Marrow Cell Responses on the Surface of Hydroxyapatite with Different Topography

2007 ◽  
Vol 361-363 ◽  
pp. 1107-1110 ◽  
Author(s):  
Xue Ning Chen ◽  
Xiang Dong Zhu ◽  
Hong Song Fan ◽  
Xing Dong Zhang
Keyword(s):  
Bone Marrow ◽  
Surface Topography ◽  
Bone Marrow Cell ◽  
Osteoblast Differentiation ◽  
Marrow Cell ◽  
Cellular Responses ◽  
Differentiation Potential ◽  
Alp Activity ◽  
Significant Difference ◽  
Rat Bone

It is well known that the cellular responses are related with both physical and chemical characteristics of substrate, including surface topography. In the present study，the effect of surface topography of hydroxyapatite (HA) on rat bone marrow cell (rBMCs) response was investigated. HA disc-shaped pellets with various topography were manufactured by single-axis pressing methods. The rBMCs responses on materials including cell morphology and proliferation were evaluated by SEM and MTT methods respectively, and the differentiation potential was assessed by total protein content and alkaline phosphatase (ALP) activity testing. The results showed that the cell porliferation was higher on HA surfaces with macropore structure, while ALP activity was lower. No significant difference in the cellular responses on the pore distribution and orietation was observed. However, the pore structure had a potential to guide cell orientation by gathering the cells inside the pores rather than on the ridges. Since ALP served as an indicator of early osteoblast differentiation, in this study its higher expression on HA surface with micropores suggested that surface microtopograhy exhibited an important effect on early osteoblast differentiation process.

The in vitro behavior of as-prepared and pre-immersed RF-sputtered calcium phosphate thin films in a rat bone marrow cell model

Biomaterials ◽  
2006 ◽  
Vol 27 (8) ◽  
pp. 1333-1340 ◽  
Author(s):  
E. van der Wal ◽  
A.M. Vredenberg ◽  
P.J. Ter Brugge ◽  
J.G.C. Wolke ◽  
John A. Jansen
Keyword(s):  
Thin Films ◽  
Bone Marrow ◽  
Calcium Phosphate ◽  
Bone Marrow Cell ◽  
Cell Model ◽  
Marrow Cell ◽  
Rat Bone

scholarly journals Transferrin promotes calcified nodule formation in rat bone marrow cell culture with dexamethasone

2019 ◽  
Vol 3 (2) ◽  
pp. 046-054
Author(s):  
Ikuo Nishikawa ◽  
Masataka Yoshikawa ◽  
Ayano Miyamoto ◽  
Hitomi Nakama ◽  
Hiroshi Maeda
Keyword(s):  
Cell Culture ◽  
Bone Marrow ◽  
Bone Marrow Cell ◽  
Marrow Cell ◽  
Nodule Formation ◽  
Bone Marrow Cell Culture ◽  
Rat Bone

Rat Bone Marrow Cell Responses on the Surface of Hydroxyapatite with Different Topography

2007 ◽  
pp. 1107-1110
Author(s):  
Xue Ning Chen ◽  
Xiang Dong Zhu ◽  
Hong Song Fan ◽  
Xing Dong Zhang
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cell ◽  
Marrow Cell ◽  
Cell Responses ◽  
Rat Bone
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