Improvement of the compressive strength of a cuttlefish bone‐derived porous hydroxyapatite scaffold via polycaprolactone coating

Porous HAp scaffolds have been prepared by using the slurry including HAp and magnesia based on the replication of polymer sponge substrate. The influence of HAp and MgO content in slurry on the pore morphology and size, and density, porosity, and mechanical strength of porous HAp scaffolds was investigated. The obtained scaffolds with average pore sizes ranging 150 to 300 μm had open, relatively uniform, and interconnected porous structure regardless of HAp and MgO content. As the MgO content increased, the pore network frame of scaffolds became to be relatively stronger, even though the pore size was not much changed. The compressive strength of the scaffolds increased rapidly with the increase of MgO content at a fixed HAp content because of increasing the pore wall thickness and density of the scaffolds. As a result, the porosity, density, and compressive strength of the porous HAp scaffolds scaffolds prepared by the sponge method were significantly affected by the addition of MgO.

Download Full-text

Enhancement of osteogenesis using a novel porous hydroxyapatite scaffold in vivo and vitro

Ceramics International ◽

10.1016/j.ceramint.2018.08.249 ◽

2018 ◽

Vol 44 (17) ◽

pp. 21656-21665 ◽

Cited By ~ 12

Author(s):

Xiaohua Ren ◽

Qiang Tuo ◽

Kun Tian ◽

Guo Huang ◽

Jinyu Li ◽

...

Keyword(s):

Porous Hydroxyapatite ◽

Hydroxyapatite Scaffold

Download Full-text

Proliferation and osteogenic differentiation of mesenchymal stromal cells in a novel porous hydroxyapatite scaffold

Regenerative Medicine ◽

10.2217/rme.15.27 ◽

2015 ◽

Vol 10 (5) ◽

pp. 579-590 ◽

Cited By ~ 13

Author(s):

Genasan Krishnamurithy ◽

Malliga Raman Murali ◽

Mohd Hamdi ◽

Azlina Amir Abbas ◽

Hanumantharao Balaji Raghavendran ◽

...

Keyword(s):

Osteogenic Differentiation ◽

Mesenchymal Stromal Cells ◽

Stromal Cells ◽

Porous Hydroxyapatite ◽

Hydroxyapatite Scaffold

Download Full-text

Improvement of compressive strength of porous hydroxyapatite scaffolds by adding polystyrene to camphene-based slurries

Materials Letters ◽

10.1016/j.matlet.2009.01.080 ◽

2009 ◽

Vol 63 (11) ◽

pp. 955-958 ◽

Cited By ~ 27

Author(s):

Se-Won Yook ◽

Hyoun-Ee Kim ◽

Byung-Ho Yoon ◽

Young-Mi Soon ◽

Young-Hag Koh

Keyword(s):

Compressive Strength ◽

Porous Hydroxyapatite

Download Full-text

Preparation of Porous Hydroxyapatite-Zirconia Composite Scaffolds by Combination of Gel-Casting and Polymer Sponge Methods

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.105-106.616 ◽

2010 ◽

Vol 105-106 ◽

pp. 616-619 ◽

Cited By ~ 2

Author(s):

Li Li Wang ◽

Xiu Feng Wang ◽

Hong Tao Jiang ◽

Cheng Long Yu

Keyword(s):

Compressive Strength ◽

Electron Microscope ◽

Porous Structure ◽

Porous Scaffolds ◽

Pore Morphology ◽

Porous Composite ◽

Composite Scaffolds ◽

Porous Hydroxyapatite ◽

Casting Technique ◽

Gel Casting

Hydroxyapatite (HA) doped with 3%yttria-stabilized 20wt% zirconia (ZrO2) ceramic were developed in order to produce a porous composite biomaterial by integrating the gel-casting technique with polymer sponge method with improved mechanical strength and controllable porous structure. The pore morphology, size, and distribution of the scaffolds were characterized using an electron microscope. The scaffolds prepared have an open, uniform and interconnected porous structure with a pore size of 300~500m. The porosity of the open pores in the scaffold can be controlled by changing HA-ZrO2 composite concentration and it is between 87%~35%. A compressive strength of 12MPa for HA-ZrO2 porous scaffolds with HA-ZrO2 concentration of 55wt% was achieved, which is comparable to that of cortical bone.

Download Full-text

Microchannel Porous Hydroxyapatite Scaffold Promotes Osteogenic Differentiation by Altering the Expression of miRNAs

10.21203/rs.3.rs-396590/v1 ◽

2021 ◽

Author(s):

Li Deng ◽

Wei Qing ◽

Lijuan Huang ◽

Cong Liu ◽

Jiajun Zheng ◽

...

Keyword(s):

Osteogenic Differentiation ◽

Expression Profiles ◽

Differentially Expressed ◽

Cell Junction ◽

Biological Processes ◽

Porous Hydroxyapatite ◽

Differentially Expressed Mirnas ◽

Hydroxyapatite Scaffold

Abstract Hydroxyapatite is a commonly used scaffold material for bone tissue engineering. However, the osteogenic mechanism of hydroxyapatite scaffolds remains unclear. Recently, we have prepared a hydroxyapatite scaffolds with microchannels and porous structures (HAG) which have good osteogenic effects in vitro and in vivo. In present study, we explained the mechanism of HAG scaffolds promoted the osteogenic differentiation from the perspective of miRNA differential expression. We used microarray assays to analyze the expression profiles of miRNAs from the osteogenic differentiation of hPMSCs with or without HAG; 16 miRNAs were upregulated and 29 miRNAs were downregulated between the two types of cells. And overexpression the differential miRNAs could promote the osteogenic differentiation of hPMSCs. Additionally, gene ontology analysis, pathway analysis, and miRNA-mRNA-network built were performed to reveal that the differentially expressed miRNAs participate in multiple biological processes, including cell metabolic, cell junction, cell development, differentiation, and signal transduction, among others. Furthermore, we found that these differentially expressed miRNAs connect osteogenic differentiation to processes such as axon guidance, MAPK, and TGF-beta signaling pathway. This is the first study to identify and characterize differentiational miRNAs derived from HAG-hPMSC cells.

Download Full-text

Optimization of Additional Composition Variations ZnO Nanoparticles on The Characteristics of Porous Hydroxypatite as Bone Filler

Indonesian Applied Physics Letters ◽

10.20473/iapl.v2i2.31699 ◽

2021 ◽

Vol 2 (2) ◽

pp. 40

Author(s):

Fardatul Azkiyah ◽

Drs. Djony Izak Rudyardjo, M.Si. ◽

Jan Ady

Keyword(s):

Compressive Strength ◽

Polyurethane Foam ◽

Zno Nanoparticles ◽

Research Process ◽

Sem Analysis ◽

Porous Hydroxyapatite ◽

Bone Filler ◽

Compressive Strength Test ◽

Injection Methods ◽

Materials Used

Research on the synthesis and characterization of porous hydroxyapatite with the addition of ZnO nanoparticles has been carried out through a combination of foam immersion and injection methods. This research was conducted to optimize the previous research by increasing the variation of ZnO composition and adding the injection method to the research process. The materials used in this research include hydroxyapatite nanoparticles, ZnO nanoparticles, Aquades, PVA and polyurethane foam. Manufacturing is done by immersing polyurethane foam into a slurry. Slurry is a mixture of PVA and hydroxyapatite solutions with variations in the addition of ZnO nanoparticles (8 wt%, 10 wt%, 12 wt%, and 14 wt%). Then inject the remaining slurry into the foam. After that the sample was dried and heated at a temperature of 650ºC to remove foam and PVA, then the sample was sintered at a temperature of 1200ºC for 3 hours. Based on SEM analysis, porosity test, and compressive strength test, the best results were shown by sample IV because it had a pore diameter of 142.9 – 371.4 m with a porosity of 69.983%, a compressive strength value of 1.8653 MPa and non-toxic. The best results have not met the standard for bone filler application. In further research, improvements need to be made by using other additives such as ZrO2, so that it can improve the mechanical properties of porous hydroxyapatite to meet standard bone filler applications.

Download Full-text