In Vivo Osteogenesis in Porous Hydroxyapatite Scaffold Processed in Hyaluronic Acid Solution

2007 ◽  
pp. 1185-1188
Author(s):  
Masataka Yoshikawa ◽  
T. Yabuuchi ◽  
N. Tsuji ◽  
Y. Shimomura ◽  
H. Hayashi ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Acid Solution ◽  
Porous Hydroxyapatite ◽  
Hydroxyapatite Scaffold

In Vivo Osteogenesis in Porous Hydroxyapatite Scaffold Processed in Hyaluronic Acid Solution

2007 ◽  
Vol 361-363 ◽  
pp. 1185-1188 ◽  
Author(s):  
Masataka Yoshikawa ◽  
T. Yabuuchi ◽  
N. Tsuji ◽  
Y. Shimomura ◽  
H. Hayashi ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Hyaluronic Acid ◽  
Acid Solution ◽  
Bone Marrow Cells ◽  
Subcutaneous Tissue ◽  
Porous Hydroxyapatite ◽  
Alp Activity ◽  
Significant Difference ◽  
Marrow Cells

Porous hydroxyapatite (HA) scaffolds were processed in hyaluronic acid solution. Bone marrow cells obtained from the bone shaft of femurs of Fischer 344 rats at 1×106/ml concentration were seeded in pores of the scaffolds. The scaffolds were implanted in the dorsal subcutaneous tissue of rats for 2, 4, 6 or 8 weeks. Removed HA scaffolds at 2 and 4 week after dorsal subcutaneous implantation were histologically examined. At all experimental periods, osteocalcin in the scaffold was immunochemically measured for the quantitative analysis of osteogenesis by bone marrow cells in the porous HA scaffolds. Moreover, value of alkaline phosphatase (ALP) activity in the scaffolds was measured. Osteocalcin measured in scaffolds without bone marrow cells was 1.3 ng in an average and the ALP activity was 62.2 μmol at 4 week. In hyaluronic acid processed scaffold with bone marrow cells, quantity of osteocalcin increased from 1.6 ng at 2 week to 2.2 ng at 4 week after implantation of the scaffold. Histologically, many pores containing bone in the scaffolds immersed in hyaluronic acid solution were detected. Significant difference of the quantity of osteocalcin was recognized between 2 and 4 week implantation. There was no significant difference in the quantity of osteocalcin between the scaffolds implanted for 4 and 8 weeks. Value of ALP activity of the scaffold implanted for 4 weeks showed significant difference comparing with that implanted for 6 and 8 weeks. From the results of this study, quantitative increase of the bone formation in the pores of HA scaffolds would be able to observe from 6 to 8 weeks after implantation on the scaffolds by immersion in hyaluronic acid solution

Enhancement of osteogenesis using a novel porous hydroxyapatite scaffold in vivo and vitro

2018 ◽  
Vol 44 (17) ◽  
pp. 21656-21665 ◽  
Author(s):  
Xiaohua Ren ◽  
Qiang Tuo ◽  
Kun Tian ◽  
Guo Huang ◽  
Jinyu Li ◽  
...  
Keyword(s):  
Porous Hydroxyapatite ◽  
Hydroxyapatite Scaffold

scholarly journals Microchannel Porous Hydroxyapatite Scaffold Promotes Osteogenic Differentiation by Altering the Expression of miRNAs

2021 ◽  
Author(s):  
Li Deng ◽  
Wei Qing ◽  
Lijuan Huang ◽  
Cong Liu ◽  
Jiajun Zheng ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
Expression Profiles ◽  
Differentially Expressed ◽  
Cell Junction ◽  
Biological Processes ◽  
Porous Hydroxyapatite ◽  
Differentially Expressed Mirnas ◽  
Hydroxyapatite Scaffold

Abstract Hydroxyapatite is a commonly used scaffold material for bone tissue engineering. However, the osteogenic mechanism of hydroxyapatite scaffolds remains unclear. Recently, we have prepared a hydroxyapatite scaffolds with microchannels and porous structures (HAG) which have good osteogenic effects in vitro and in vivo. In present study, we explained the mechanism of HAG scaffolds promoted the osteogenic differentiation from the perspective of miRNA differential expression. We used microarray assays to analyze the expression profiles of miRNAs from the osteogenic differentiation of hPMSCs with or without HAG; 16 miRNAs were upregulated and 29 miRNAs were downregulated between the two types of cells. And overexpression the differential miRNAs could promote the osteogenic differentiation of hPMSCs. Additionally, gene ontology analysis, pathway analysis, and miRNA-mRNA-network built were performed to reveal that the differentially expressed miRNAs participate in multiple biological processes, including cell metabolic, cell junction, cell development, differentiation, and signal transduction, among others. Furthermore, we found that these differentially expressed miRNAs connect osteogenic differentiation to processes such as axon guidance, MAPK, and TGF-beta signaling pathway. This is the first study to identify and characterize differentiational miRNAs derived from HAG-hPMSC cells.

scholarly journals Physico-chemical and Histomorphometric Evaluation of Zinc-containing Hydroxyapatite in Rabbits Calvaria

2016 ◽  
Vol 27 (6) ◽  
pp. 717-726 ◽  
Author(s):  
Alinne Azevedo Pereira da Silva Suruagy ◽  
Adriana Terezinha Neves Novellino Alves ◽  
Suelen Cristina Sartoretto ◽  
José de Albuquerque Calasans-Maia ◽  
José Mauro Granjeiro ◽  
...  
Keyword(s):  
Bone Repair ◽  
Chemical Properties ◽  
Wilcoxon Test ◽  
In Vivo Studies ◽  
Control Group ◽  
Porous Hydroxyapatite ◽  
Physico Chemical ◽  
Hydroxyapatite Scaffold ◽  
Xrd Patterns

Abstract The aim of this study was to characterize the physico-chemical properties and bone repair after implantation of zinc-containing nanostructured porous hydroxyapatite scaffold (nZnHA) in rabbits' calvaria. nZnHA powder containing 2% wt/wt zinc and stoichiometric nanostructured porous hydroxyapatite (nHA - control group) were shaped into disc (8 mm) and calcined at 550 °C. Two surgical defects were created in the calvaria of six rabbits (nZnHA and nHA). After 12 weeks, the animals were euthanized and the grafted area was removed, fixed in 10% formalin with 0.1 M phosphate buffered saline and embedded in paraffin (n=10) for histomorphometric evaluation. In addition, one sample from each group (n=2) was embedded in methylmethacrylate for the SEM and EDS analyses. The thermal treatment transformed the nZnHA disc into a biphasic implant composed of Zn-containing HA and Zn-containing β-tricalcium phosphate (ZnHA/βZnTCP). The XRD patterns for the nHA disc were highly crystalline compared to the ZnHA disc. Histological analysis revealed that both materials were biologically compatible and promoted osteoconduction. X-ray fluorescence and MEV-EDS of nZnHA confirmed zinc in the samples. Histomorphometric evaluation revealed the presence of new bone formation in both frameworks but without statistically significant differences (p>0.05), based on the Wilcoxon test. The current study confirmed that both biomaterials improve bone repair, are biocompatible and osteoconductive, and that zinc (2wt%) did not increase the bone repair. Additional in vivo studies are required to investigate the effect of doping hydroxyapatite with a higher Zn concentration.

scholarly journals Physico-Chemical, In Vitro, and In Vivo Evaluation of a 3D Unidirectional Porous Hydroxyapatite Scaffold for Bone Regeneration

Materials ◽  
2017 ◽  
Vol 10 (1) ◽  
pp. 33 ◽  
Author(s):  
Manabu Tanaka ◽  
Hisao Haniu ◽  
Takayuki Kamanaka ◽  
Takashi Takizawa ◽  
Atsushi Sobajima ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
In Vivo Evaluation ◽  
Porous Hydroxyapatite ◽  
Physico Chemical ◽  
Hydroxyapatite Scaffold

scholarly journals Secreted KIAA1199 promotes the progression of rheumatoid arthritis by mediating hyaluronic acid degradation in an ANXA1-dependent manner

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Wei Zhang ◽  
Guoyu Yin ◽  
Heping Zhao ◽  
Hanzhi Ling ◽  
Zhen Xie ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Hyaluronic Acid ◽  
Dependent Manner ◽  
Collagen Induced Arthritis ◽  
Intracellular Degradation ◽  
Main Pathway ◽  
First Time

AbstractIn inflamed joints, enhanced hyaluronic acid (HA) degradation is closely related to the pathogenesis of rheumatoid arthritis (RA). KIAA1199 has been identified as a hyaladherin that mediates the intracellular degradation of HA, but its extracellular function remains unclear. In this study, we found that the serum and synovial levels of secreted KIAA1199 (sKIAA1199) and low-molecular-weight HA (LMW-HA, MW < 100 kDa) in RA patients were significantly increased, and the positive correlation between them was shown for the first time. Of note, treatment with anti-KIAA1199 mAb effectively alleviated the severity of arthritis and reduced serum LMW-HA levels and cytokine secretion in collagen-induced arthritis (CIA) mice. In vitro, sKIAA1199 was shown to mediate exogenous HA degradation by attaching to the cell membrane of RA fibroblast-like synoviosytes (RA FLS). Furthermore, the HA-degrading activity of sKIAA1199 depended largely on its adhesion to the membrane, which was achieved by its G8 domain binding to ANXA1. In vivo, kiaa1199-KO mice exhibited greater resistance to collagen-induced arthritis. Interestingly, this resistance could be partially reversed by intra-articular injection of vectors encoding full-length KIAA1199 instead of G8-deleted KIAA119 mutant, which further confirmed the indispensable role of G8 domain in KIAA1199 involvement in RA pathological processes. Mechanically, the activation of NF-κB by interleukin-6 (IL-6) through PI3K/Akt signaling is suggested to be the main pathway to induce KIAA1199 expression in RA FLS. In conclusion, our study supported the contribution of sKIAA1199 to RA pathogenesis, providing a new therapeutic target for RA by blocking sKIAA1199-mediated HA degradation.

scholarly journals Hyaluronic Acid-Coated MTX-PEI Nanoparticles for Targeted Rheumatoid Arthritis Therapy

Crystals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 321
Author(s):  
Shenghui Zhong ◽  
Peng Liu ◽  
Jinsong Ding ◽  
Wenhu Zhou
Keyword(s):  
Rheumatoid Arthritis ◽  
Hyaluronic Acid ◽  
Targeted Delivery ◽  
High Dose ◽  
One Pot ◽  
Inflammatory Site ◽  
Pei Nanoparticles ◽  
Toxic Reactions

Methotrexate (MTX) is an anchor drug for the treatment of rheumatoid arthritis (RA); however, long-term and high-dose usage of MTX for patients can cause many side effects and toxic reactions. To address these difficulties, selectively delivering MTX to the inflammatory site of a joint is promising in the treatment of RA. In this study, we prepared MTX-PEI@HA nanoparticles (NPs), composed of hyaluronic acid (HA) as the hydrophilic negative electrical shell, and MTX-linked branched polyethyleneimine (MTX-PEI) NPs as the core. MTX-PEI@HA NPs were prepared in the water phase by a one-pot method. The polymeric NPs were selectively internalized via CD44 receptor-mediated endocytosis in the activated macrophages. In the in vivo mice mode study, treatment with MTX-PEI@HA NPs mitigated inflammatory arthritis with notable safety at a high dose of MTX. We highlight the distinct advantages of aqueous-synthesized NPs coated with HA for arthritis-selective targeted delivery, thus verifying MTX-PEI@HA NPs as a promising MTX-based nanoplatform for treatment of RA.

scholarly journals Injection of a polymerized hyaluronic acid/collagen hydrogel matrix in an in vivo porcine disc degeneration model

2012 ◽  
Vol 21 (9) ◽  
pp. 1700-1708 ◽  
Author(s):  
G. W. Omlor ◽  
A. G. Nerlich ◽  
H. Lorenz ◽  
T. Bruckner ◽  
W. Richter ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Disc Degeneration ◽  
Collagen Hydrogel ◽  
Hydrogel Matrix

scholarly journals In vitro and in vivo effect of hyaluronic acid modified, doxorubicin and gallic acid co-delivered lipid-polymeric hybrid nano-system for leukemia therapy

2019 ◽  
Vol Volume 13 ◽  
pp. 2043-2055 ◽  
Author(s):  
Yanping Shao ◽  
Wenda Luo ◽  
Qunyi Guo ◽  
Xiaohong Li ◽  
Qianqian Zhang ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Gallic Acid

Encapsulated protamine-hyaluronic acid particles for targeting carboplatin directed by radiation

2017 ◽  
Vol 27 (01n02) ◽  
pp. 37-42
Author(s):  
T. Segawa ◽  
S. Harada ◽  
S. Ehara ◽  
K. Ishii ◽  
T. Sato ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Radiation Dose ◽  
Cancer Treatment ◽  
Standard Error ◽  
Room Temperature ◽  
Pixe Analysis ◽  
X Ray ◽  
Clinical Cancer

Encapsulated protamine-hyaluronic acid particles containing carboplatin were prepared and their ability to release carboplatin was tested in vivo. Protamine–hyaluronic acid particles containing carboplatin were prepared by mixing protamine (1.6 mg) and hyaluronic acid (1.28 mg) into a 5 mg/mL carboplatin solution for 30 min at room temperature. A 1 mL solution of protamine–hyaluronic acid particles was poured into an ampule of COATSOME[Formula: see text] EL-010 (Nichiyu, Tokyo, Japan), shaken three times by hand, and allowed to incubate at room temperature for 15 min. Following that, 10 or 20 Gy of 100 kiloelectronvolt (KeV) soft X-ray was applied. The release of carboplatin was imaged using a microparticle-induced X-ray emission (PIXE) camera. The amount of carboplatin released was expressed as the amount of platinum released and measured via quantitative micro-PIXE analysis. The diameter of the generated encapsulated particles measured [Formula: see text] nm (mean ± standard error). The release of carboplatin from the encapsulated protamine–hyaluronic acid particles was observed under a micro-PIXE camera. The amount of carboplatin released was [Formula: see text] under 10 Gy of radiation, and [Formula: see text] under 20 Gy of radiation, which was a sufficient dose for cancer treatment. However, 10 or 20 Gy of radiation is much greater than the dose used for clinical cancer treatment (2 Gy). Further research to reduce the radiation dose to 2 Gy in order to release sufficient carboplatin for cancer treatment is required.

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