Micro-CT and PET analysis of bone regeneration induced by biodegradable scaffolds as carriers for dental pulp stem cells in a rat model of calvarial “critical size” defect: Preliminary data

This is preliminary study in order to investigate the effect of dental pulp stem cells (DPSCs) on bone regeneration in an animal model. New Zealand rabbits were used as animal model. The critical defect was created in femoral bone and transplantation of DPSCs applied into bone defect. A colorimetric assay was used to detect ALP level in rabbit’s serum. Bone tissue regeneration was evaluated by histological analysis. In the 2nd week, the treated rabbit show increasing in the activity of ALP (157,925 μU) compared to control rabbit (155,361 μU). This increasing trend continues significantly in DPSCs rabbit (169.750 μU) compared to control rabbit (160.406) after 4 weeks. Histological evaluation revealed that the amount of bone lamellae and osteocytes were filled the defect area of DPSCs treated rabbit. Conclusions: Transplantation of DPSCs accelerating bone regeneration by raising ALP level and forming new bone tissue.

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Bone regeneration by human dental pulp stem cells using a helioxanthin derivative and cell-sheet technology

Stem Cell Research & Therapy ◽

10.1186/s13287-018-0783-7 ◽

2018 ◽

Vol 9 (1) ◽

Cited By ~ 18

Author(s):

Yasuyuki Fujii ◽

Yoko Kawase-Koga ◽

Hironori Hojo ◽

Fumiko Yano ◽

Marika Sato ◽

...

Keyword(s):

Stem Cells ◽

Bone Regeneration ◽

Dental Pulp ◽

Cell Sheet ◽

Dental Pulp Stem Cells ◽

Human Dental Pulp ◽

Cell Sheet Technology

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Effect of extracellular matrix and dental pulp stem cells on bone regeneration with 3D printed PLA/HA composite scaffolds

10.22203/ecm.v041a15 ◽

2021 ◽

Vol 41 ◽

pp. 204-215

Author(s):

I Gendviliene ◽

◽

E Simoliunas ◽

M Alksne ◽

S Dibart ◽

...

Keyword(s):

Stem Cells ◽

Extracellular Matrix ◽

Bone Regeneration ◽

Dental Pulp ◽

Negative Control ◽

Dental Pulp Stem Cells ◽

Female Rats ◽

Micro Computed Tomography ◽

Significant Difference ◽

Gender Specific Differences

The demand for bone grafting procedures in various fields of medicine is increasing. Existing substitutes in clinical practice do not meet all the criteria required for an ideal bone scaffold, so new materials are being sought. This study evaluated bone regeneration using a critical-size Wistar rat’s calvarial defect model. 12 male and 12 female rats were evenly divided into 3 groups: 1. Negative and positive (Geistlich Bio-Oss®) controls; 2. polylactic acid (PLA) and PLA/hydroxyapatite (HA); 3. PLA/HA cellularised with dental pulp stem cells (DPSC) and PLA/HA extracellular matrix (ECM) scaffolds. PLA/HA filament was created using hot-melt extrusion equipment. All scaffolds were fabricated using a 3D printer. DPSC were isolated from the incisors of adult Wistar rats. The defects were evaluated by micro-computed tomography (µCT) and histology, 8 weeks after surgery. µCT revealed that the Bio-Oss group generated 1.49 mm3 and PLA/HA ECM 1.495 mm3 more bone volume than the negative control. Histology showed a statistically significant difference between negative control and both (Bio-Oss and PLA/HA ECM) groups in rats of both genders. Moreover, histology showed gender-specific differences in all experimental groups and a statistically significant difference between cellularised PLA/HA and PLA/HA ECM groups in female rats. Qualitative histology showed the pronounced inflammation reaction during biodegradation in the PLA group. In conclusion, the bone-forming ability was comparable between the Bio-Oss and PLA/HA ECM scaffolds. Further research is needed to analyse the effects of ECM and PLA/HA ratio on osteoregeneration.

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