Gingival fibroblasts are better at inhibiting osteoclast formation than periodontal ligament fibroblasts

2006 ◽  
Vol 98 (2) ◽  
pp. 370-382 ◽  
Author(s):  
Teun J. de Vries ◽  
Ton Schoenmaker ◽  
Nutthamon Wattanaroonwong ◽  
Marije van den Hoonaard ◽  
Arlies Nieuwenhuijse ◽  
...  
Keyword(s):  
Periodontal Ligament ◽  
Osteoclast Formation ◽  
Gingival Fibroblasts ◽  
Periodontal Ligament Fibroblasts

Identification of Genes Differentially Expressed in Cultured Human Periodontal Ligament Fibroblasts vs. Human Gingival Fibroblasts by DNA Microarray Analysis

2002 ◽  
Vol 81 (6) ◽  
pp. 399-405 ◽  
Author(s):  
X. Han ◽  
S. Amar
Keyword(s):  
Gene Expression ◽  
Dna Microarray ◽  
Periodontal Ligament ◽  
Expression Patterns ◽  
Differentially Expressed ◽  
Gingival Fibroblasts ◽  
Periodontal Ligament Fibroblasts ◽  
Genes Encoding ◽  
Different Characteristics ◽  
Related Proteins

Despite their similar spindle-shaped appearance, periodontal ligament fibroblasts (PDLF) and gingival fibroblasts (GF) appear to display distinct functional activities in the maintenance of tissue integrity and during inflammatory/immune responses. We postulated that different characteristics of PDLF and GF are defined by the differential expression of specific genes. To test this, we investigated the possible variance of gene expression profile between cultured PDLF and GF, using DNA microarray technology. One hundred sixty-three genes were found differentially expressed by at least three-fold between PDLF and GF. Genes encoding transmembrane proteins and cytoskeleton-related proteins tended to be up-regulated in PDLF, whereas genes encoding cell-cycle regulation proteins and metabolism-related proteins tended to be up-regulated in GF. We concluded that PDLF and GF appear to display different gene expression patterns that may reflect intrinsic functional differences of the two cell populations and may well coordinate with their tissue-specific activities.

Gene Expression and Accumulation of Fibrillin-1, Fibrillin-2, and Tropoelastin in Cultured Periodontal Fibroblasts

2002 ◽  
Vol 81 (11) ◽  
pp. 771-775 ◽  
Author(s):  
E. Tsuruga ◽  
K. Irie ◽  
T. Yajima
Keyword(s):  
Gene Expression ◽  
Periodontal Ligament ◽  
Northern Blot Analysis ◽  
Elastic System ◽  
Elastic Fibers ◽  
Gingival Fibroblasts ◽  
Periodontal Ligament Fibroblasts ◽  
Human Gingiva ◽  
Fibrillin 1 ◽  
Cell Layers

The elastic system fibers consist of three types—oxytalan, elaunin, and elastic fibers—differing in their relative microfibril and elastin contents. All three types are found in human gingiva, but human periodontal ligaments contain only elastin-free fibers. We examined cultured human gingival fibroblasts (HGF) and cultured human periodontal ligament fibroblasts (HPLF) to determine the gene expression of fibrillin-1 and fibrillin-2 (the major components of microfibrils) and of tropoelastin. In addition, we assessed the degree of accumulation of these proteins in the extracellular matrix. Northern blot analysis revealed that the level of expression of fibrillin-1 and fibrillin-2 was higher in HGF than in HPLF. However, examination of matrix samples from HGF and HPLF cell layers showed that there was no difference in fibrillin-1 accumulation, although fibrillin-2 accumulated to a much greater extent in the HGF-derived matrix. Tropoelastin was expressed only in and around HGF. These results show a correlation between gene expression and the accumulation of tropoelastin and fibrillin-2 in HGF.

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