Comparative peptidomic profile between human hypertrophic scar tissue and matched normal skin for identification of endogenous peptides involved in scar pathology

2018 ◽  
Vol 233 (8) ◽  
pp. 5962-5971 ◽  
Author(s):  
Jingyun Li ◽  
Ling Chen ◽  
Qian Li ◽  
Jing Cao ◽  
Yanli Gao ◽  
...  
Keyword(s):  
Hypertrophic Scar ◽  
Normal Skin ◽  
Scar Tissue ◽  
Endogenous Peptides ◽  
Hypertrophic Scar Tissue

scholarly journals iTRAQ-based proteomic profiling reveals different protein expression between normal skin and hypertrophic scar tissue

2015 ◽  
Vol 3 ◽  
pp. 1-8 ◽  
Author(s):  
Jianglin Tan ◽  
Weifeng He ◽  
Gaoxing Luo ◽  
Jun Wu
Keyword(s):  
Hypertrophic Scar ◽  
Normal Skin ◽  
Scar Tissue ◽  
Proteomic Profiling ◽  
Absolute Quantitation ◽  
Burn Care ◽  
Surgical Methods ◽  
Differential Proteins ◽  
Isobaric Tags ◽  
Hypertrophic Scar Tissue

Abstract Background A hypertrophic scar is a unique fibrotic disease that only exists in humans. Despite advances in burn care and rehabilitation, as well as progress in the management during these decades, the hypertrophic scar remains hard to cure following surgical methods and drugs for treatment. In this study, we are looking forward to finding the multitude of possible traumatic mechanisms and the underlying molecular signal ways in the formation of the hypertrophic scar. Methods We used isobaric tags for relative and absolute quantitation (iTRAQ) labeling technology, followed by high-throughput 2D LC-MS/MS, to determine relative quantitative differential proteins between the hypertrophic scar and normal skin tissue. Results A total of 3166 proteins were identified with a high confidence (≥95 % confidence). And, a total of 89 proteins were identified as the differential proteins between the hypertrophic scar and normal skin, among which 41 proteins were up-regulated and 48 proteins were down-regulated in the hypertrophic scar. GO-Analysis indicated the up-regulated proteins were involved in extracellular matrix, whereas the down-regulated proteins were involved in dynamic junction and structural molecule activity. Conclusions In our study, we demonstrate 89 proteins present differently in the hypertrophic scar compared to normal skin by iTRAQ technology, which might indicate the pathologic process of hypertrophic scar formation and guide us to propose new strategies against the hypertrophic scar.

NON-LINEAR SPECTRAL IMAGING MICROSCOPY STUDIES OF HUMAN HYPERTROPHIC SCAR

2009 ◽  
Vol 02 (01) ◽  
pp. 61-66 ◽  
Author(s):  
KECHENG LU ◽  
SHUANGMU ZHUO ◽  
ZHIBIN HONG ◽  
GUANNAN CHEN ◽  
XINGSHAN JIANG ◽  
...  
Keyword(s):  
Hypertrophic Scar ◽  
Normal Skin ◽  
Spectral Characteristics ◽  
Morphological Structure ◽  
Spectral Imaging ◽  
Scar Tissue ◽  
Elastic Fibers ◽  
Non Linear ◽  
Hypertrophic Scar Tissue ◽  
Skin Scar

Skin scar is unique to humans, the major significant negative outcome sustained after thermal injuries, traumatic injuries, and surgical procedures. Hypertrophic scar in human skin is investigated using non-linear spectral imaging microscopy. The high contrast images and spectroscopic intensities of collagen and elastic fibers extracted from the spectral imaging of normal skin tissue, and the normal skin near and far away from the hypertrophic scar tissues in a 10-year-old patient case are obtained. The results show that there are apparent differences in the morphological structure and spectral characteristics of collagen and elastic fibers when comparing the normal skin with the hypertrophic scar tissue. These differences can be good indicators to differentiate the normal skin and hypertrophic scar tissue and demonstrate that non-linear spectral imaging microscopy has potential to noninvasively investigate the pathophysiology of human hypertrophic scar.

scholarly journals Loureirin B Inhibits Hypertrophic Scar Formation via Inhibition of the TGF-β1-ERK/JNK Pathway

2015 ◽  
Vol 37 (2) ◽  
pp. 666-676 ◽  
Author(s):  
Ting He ◽  
Xiaozhi Bai ◽  
Longlong Yang ◽  
Lei Fan ◽  
Yan Li ◽  
...  
Keyword(s):  
Hypertrophic Scar ◽  
Ex Vivo ◽  
Scar Tissue ◽  
Scar Formation ◽  
Jnk Pathway ◽  
Protein Levels ◽  
Mapk Inhibitors ◽  
Hypertrophic Scar Tissue ◽  
Loureirin B ◽  
Tgf Β1

Background/Aims: Our previous study confirmed that Loureirin B (LB) can inhibit hypertrophic scar formation. However, the mechanism of LB-mediated inhibition of scar formation is still unknown. Methods: Immunohistochemistry was used to detect expression of Col1, FN and TGF-β1 in skin and scar tissue. Fibroblasts were stimulated with TGF-β1 to mimic scar formation. LB or MAPK inhibitors were used to study the pathways involved in the process. Western blotting was used to evaluate the expression of p-JNK, p-ERK, p-p38, Col1 and FN. The contractile capacity of fibroblasts was evaluated using a gel contraction assay. Tissues were cultured ex vivo with LB to further investigate the participation of ERK and JNK in the LB-mediated inhibition of scar formation. Results: FN and Col1 were up regulated in hypertrophic scars. LB down regulated p-ERK and p-JNK in TGF-β1-stimulated fibroblasts, while levels of phosphorylated p38 did not change. The down regulation of p-ERK and p-JNK was associated with a reduction of Col1 and FN. Similarly, inhibition of ERK and JNK down regulated the expression of Col1 and FN in TGF-β1-stimulated fibroblasts. LB down regulated protein levels of p-ERK and p-JNK in cultured hypertrophic scar tissue ex vivo. Conclusions: This study suggests that LB can inhibit scar formation through the ERK/JNK pathway.

Enhanced expression of mRNA for insulin-like growth factor-1 in post-burn hypertrophic scar tissue and its fibrogenic role by dermal fibroblasts

1995 ◽  
Vol 148 (1) ◽  
pp. 25-32 ◽  
Author(s):  
Aziz Ghahary ◽  
You J. Shen ◽  
Bernadette Nedelec ◽  
Paul G. Scott ◽  
Edward E. Tredget
Keyword(s):  
Growth Factor ◽  
Hypertrophic Scar ◽  
Scar Tissue ◽  
Dermal Fibroblasts ◽  
Insulin Like Growth Factor ◽  
Enhanced Expression ◽  
Hypertrophic Scar Tissue

The Use of Silicone Gel Sheets in the Control of Hypertrophic Scar Tissue

1988 ◽  
Vol 51 (7) ◽  
pp. 248-249 ◽  
Author(s):  
Rosemary Gollop
Keyword(s):  
Retrospective Study ◽  
Hypertrophic Scar ◽  
Scar Tissue ◽  
City Hospital ◽  
Hypertrophic Scarring ◽  
The Past ◽  
Silicone Gel ◽  
Clinical Observations ◽  
Hypertrophic Scar Tissue ◽  
Indications For Use

For the past 3 years, Nottingham City Hospital Burns and Plastic Surgery Unit have been using silicone gel in addition to, or as an alternative to, accepted methods of treating hypertrophic scarring. This paper outlines the history, properties and indications for use of silicone gel. Its findings result from a retrospective study based largely on clinical observations.

In vitro study of ethosome penetration in human skin and hypertrophic scar tissue

2012 ◽  
Vol 8 (6) ◽  
pp. 1026-1033 ◽  
Author(s):  
Zhen Zhang ◽  
Yan Wo ◽  
Yixin Zhang ◽  
Danru Wang ◽  
Rong He ◽  
...  
Keyword(s):  
Human Skin ◽  
Hypertrophic Scar ◽  
In Vitro Study ◽  
Scar Tissue ◽  
Vitro Study ◽  
Hypertrophic Scar Tissue

Reduced collagenase gene expression in fibroblasts from hypertrophic scar tissue

1996 ◽  
Vol 134 (5) ◽  
pp. 863-868 ◽  
Author(s):  
M. ARAKAWA ◽  
A. HATMOCHI ◽  
Y. MORI ◽  
K. MORI ◽  
H. UEKI ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hypertrophic Scar ◽  
Scar Tissue ◽  
Hypertrophic Scar Tissue ◽  
Collagenase Gene

scholarly journals High-Efficiency Expression of TAT-bFGF Fusion Protein in Escherichia coli and the Effect on Hypertrophic Scar Tissue

PLoS ONE ◽  
2015 ◽  
Vol 10 (2) ◽  
pp. e0117448 ◽  
Author(s):  
Xuechao Jia ◽  
Haishan Tian ◽  
Lu Tang ◽  
Long Zheng ◽  
Lulu Zheng ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fusion Protein ◽  
Hypertrophic Scar ◽  
High Efficiency ◽  
Scar Tissue ◽  
Hypertrophic Scar Tissue

scholarly journals Nitric Oxide Synthase Expression and Nitric Oxide Production Are Reduced in Hypertrophic Scar Tissue and Fibroblasts

1997 ◽  
Vol 108 (4) ◽  
pp. 438-444 ◽  
Author(s):  
Rijian Wang ◽  
Aziz Ghahary ◽  
You J Shen ◽  
Paul G Scott ◽  
Edward E Tredget
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Hypertrophic Scar ◽  
Scar Tissue ◽  
Nitric Oxide Production ◽  
Oxide Production ◽  
Hypertrophic Scar Tissue ◽  
Oxide Synthase
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