Autophagy displays divergent roles during intermittent amino acid starvation and toxic stress‐induced senescence in cultured skeletal muscle cells

2020 ◽  
Author(s):  
Darin Bloemberg ◽  
Joe Quadrilatero
Keyword(s):  
Skeletal Muscle ◽  
Amino Acid ◽  
Muscle Cells ◽  
Amino Acid Starvation ◽  
Skeletal Muscle Cells ◽  
Toxic Stress

Ceramide down‐regulates System A amino acid transport and protein synthesis in rat skeletal muscle cells

2004 ◽  
Vol 19 (3) ◽  
pp. 1-24 ◽  
Author(s):  
Russell Hyde ◽  
Eric Hajduch ◽  
Darren J. Powell ◽  
Peter M. Taylor ◽  
Harinder S. Hundal
Keyword(s):  
Skeletal Muscle ◽  
Protein Synthesis ◽  
Amino Acid ◽  
Amino Acid Transport ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Acid Transport ◽  
Rat Skeletal Muscle ◽  
System A

Glucose and amino acid metabolism in an established line of skeletal muscle cells

1978 ◽  
Vol 96 (3) ◽  
pp. 309-317 ◽  
Author(s):  
William M. Pardridge ◽  
Mayer B. Davidson ◽  
Delia Casanello-Ertl
Keyword(s):  
Skeletal Muscle ◽  
Amino Acid ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Established Line

Energy metabolism profile of the effects of amino acid treatment on skeletal muscle cells: Leucine inhibits glycolysis of myotubes

Nutrition ◽  
2020 ◽  
Vol 77 ◽  
pp. 110794 ◽  
Author(s):  
Reiko Suzuki ◽  
Yoriko Sato ◽  
Kodwo Amuzuah Obeng ◽  
Daisuke Suzuki ◽  
Yusuke Komiya ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Amino Acid ◽  
Energy Metabolism ◽  
Acid Treatment ◽  
Muscle Cells ◽  
Skeletal Muscle Cells

scholarly journals Critical Amino Acid Residues Determine the Binding Affinity and the Ca2+ Release Efficacy of Maurocalcine in Skeletal Muscle Cells

2003 ◽  
Vol 278 (39) ◽  
pp. 37822-37831 ◽  
Author(s):  
Eric Estève ◽  
Sophia Smida-Rezgui ◽  
Sandor Sarkozi ◽  
Csaba Szegedi ◽  
Imed Regaya ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Amino Acid ◽  
Binding Affinity ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Amino Acid Residues ◽  
Critical Amino Acid

Effects of clofibric acid on amino acid metabolism in cultured rat skeletal muscle

1981 ◽  
Vol 240 (2) ◽  
pp. E203-E208
Author(s):  
W. M. Pardridge ◽  
L. Duducgian-Vartavarian ◽  
D. Casanello-Ertl ◽  
M. R. Jones ◽  
J. D. Kopple
Keyword(s):  
Skeletal Muscle ◽  
Amino Acid ◽  
Muscle Cells ◽  
Clofibric Acid ◽  
Skeletal Muscle Cells ◽  
Adult Rats ◽  
Carbohydrate Utilization ◽  
Branched Chain Amino Acid ◽  
Amino Acid Utilization ◽  
Branched Chain

Well-differentiated cultured skeletal muscle cells (myotubes) obtained from adult rats were incubated for up to 48 h in Dulbecco's modified Eagle's medium. Medium glucose decreased from 4.9 +/- 0.1 mM at 0 h to 13 +/- 1 microM by 24 h; approximately 60% of glucose was converted to lactate. Pyruvate, alanine, and citrate were continuously produced, even during the period of 24-48 h when no glucose or lactate utilization was observed. Branched-chain amino acid utilization increased more than fourfold during the incubation period of 24-48 h; during this time, intracellular ATP, pyruvate, alpha-ketoglutarate, malate, and citrate levels were constant despite the absence of glucose or lactate consumption. Incubation of muscle cells with 2 mM clofibric acid resulted in a 76% inhibition of leucine metabolism. Coincident with the drug-induced inhibition of a branched-chain amino acid utilization, alanine and citrate production was blocked, and cell levels of pyruvate, alpha-ketoglutarate, malate, and citrate were markedly reduced. These studies suggest branched-chain amino acids contribute significantly to anaplerotic pathways in cultured skeletal muscle cells and that these pathways lead to the net production of alanine and citrate during periods of minimal carbohydrate utilization.

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