Metagenomic insights into the microbial community and biogas production pattern during anaerobic digestion of cow dung and mixed food waste

2019 ◽  
Vol 95 (1) ◽  
pp. 151-162 ◽  
Author(s):  
Mashudu Mukhuba ◽  
Ashira Roopnarain ◽  
Mokhele Edmond Moeletsi ◽  
Rasheed Adeleke
Keyword(s):  
Microbial Community ◽  
Anaerobic Digestion ◽  
Food Waste ◽  
Biogas Production ◽  
Cow Dung ◽  
Production Pattern

scholarly journals Effect of co-digestion of food waste and cow dung on biogas yield

2020 ◽  
Vol 181 ◽  
pp. 01005
Author(s):  
Makhura Emmanuel Pax ◽  
Edison Muzenda ◽  
Tumeletso Lekgoba
Keyword(s):  
Anaerobic Digestion ◽  
Moisture Content ◽  
Food Waste ◽  
Biogas Production ◽  
Cow Dung ◽  
Mixing Ratio ◽  
Total Solids ◽  
Biogas Yield ◽  
Volatile Solids ◽  
Thermophilic Conditions

This paper aims at finding the effect of co-digestion of cow dung and food waste on total biogas yield. Biogas production was improved through co-digestion of cow dung and food waste (FW) containing a small fraction of inoculum under mesophilic temperature (37ºC) over a retention time of 24 days. Co-digestion ratios of 1:1, 2:1 and 3:1 for cowdung/foodwaste were used for the study on anaerobic digestion on the co digested matter. Tests were carried out starting with the preparation of substrates, substrate characterization to determine the moisture content (MC), total solids (TS), volatile solids (VS) and ultimately batch anaerobic digestion experiments under thermophilic conditions (370C). The moisture content, volatile solids and total solids for food waste were 78, 22 and 90.7% respectively while the characteristics for cow dung were 67.2, 32.8 and 96.0 % respectively. From the study, a mixing ratio of cow dung: food waste of 1:2 was found to be the optimum substrate mixture for biogas production at 25595.7 Nml. The accumulated gas volumes of 18756.6, 14042.5, 13940.8 and 13839.1 Nml were recorded for cow dung: food waste ratios of 2:1, 1:1, 1:3 and 3:1 respectively. For a co-digestion containing more of the food waste than cow dung, a higher volume of biogas is produce.

scholarly journals Effect of Iron Supplementation On The Biogas Production and Microbial Community Distribution During Anaerobic Digestion of Food Waste Process

2021 ◽  
Author(s):  
Yue Xu ◽  
Rongtang Zhang ◽  
Jiesheng Liu ◽  
Xinghua He ◽  
Haijun Lu ◽  
...  
Keyword(s):  
Microbial Community ◽  
Anaerobic Digestion ◽  
Food Waste ◽  
Iron Supplementation ◽  
Volatile Fatty Acids ◽  
High Throughput Sequencing ◽  
High Efficiency ◽  
Biogas Production ◽  
Iron Concentration ◽  
Two Samples

Abstract Iron as micronutrients is of great significance for forming a stable and efficient anaerobic digestion of food waste. Aim of this study was to examine the effect of iron supplementation on the mesophilic anaerobic digestion of food waste. Firstly, batch experiments were conducted with different iron concentration at a constant kitchen waste/inoculum ratio (K/I = 1.0), which indicated that the effect of iron on anaerobic digestion of was strictly dosage-dependent. Then, anaerobic digestion of food waste was conducted for 50 days in semi-continuous rectors with optimal iron concentration (2.0mg /L) under the same conditions. And the semi-continuous rectors obtained a good operation performance with low volatile fatty acids concentration, higher biogas production, high coenzyme F420 and dehydrogenase concentrations. Furthermore, two samples taken on 7th day and 50th day were analyzed by high-throughput sequencing, which illustrated that the composition anaerobe community was stable. However, the growth and activity of several syntrophic microbial groups (Aminobacterium, Syntrophomonas, Anaerolineaceae, Methanosaeta, Methanosarcina, Methanobacterium and Methanospirillum), were stimulated by iron supplementation. The shift of microbial community suggested that a high-efficiency microbial community for methane production from food waste was formed by iron supplementation.

scholarly journals Biogas potential of organic waste onboard cruise ships — a yet untapped energy source

2021 ◽  
Author(s):  
Kai Schumüller ◽  
Dirk Weichgrebe ◽  
Stephan Köster
Keyword(s):  
Energy Source ◽  
Anaerobic Digestion ◽  
Sewage Sludge ◽  
Food Waste ◽  
Energy Demand ◽  
Organic Waste ◽  
Biogas Production ◽  
Biogas Yield ◽  
Cruise Ships ◽  
Detailed Presentation

AbstractTo tap the organic waste generated onboard cruise ships is a very promising approach to reduce their adverse impact on the maritime environment. Biogas produced by means of onboard anaerobic digestion offers a complementary energy source for ships’ operation. This report comprises a detailed presentation of the results gained from comprehensive investigations on the gas yield from onboard substrates such as food waste, sewage sludge and screening solids. Each person onboard generates a total average of about 9 kg of organic waste per day. The performed analyses of substrates and anaerobic digestion tests revealed an accumulated methane yield of around 159 L per person per day. The anaerobic co-digestion of sewage sludge and food waste (50:50 VS) emerged as particularly effective and led to an increased biogas yield by 24%, compared to the mono-fermentation. In the best case, onboard biogas production can provide an energetic output of 82 W/P, on average covering 3.3 to 4.1% of the total energy demand of a cruise ship.

Long-term high-solids anaerobic digestion of food waste: Effects of ammonia on process performance and microbial community

2018 ◽  
Vol 262 ◽  
pp. 148-158 ◽  
Author(s):  
Xuya Peng ◽  
ShangYi Zhang ◽  
Lei Li ◽  
Xiaofei Zhao ◽  
Yao Ma ◽  
...  
Keyword(s):  
Microbial Community ◽  
Anaerobic Digestion ◽  
Food Waste ◽  
Process Performance ◽  
High Solids

Cultured and Uncultured Microbial Community Associated With Biogas Production

2021 ◽  
Author(s):  
Júlia Ronzella Ottoni ◽  
Suzan Prado Fernandes Bernal ◽  
Tiago Joelzer Marteres ◽  
Franciele Natividade Luiz ◽  
Viviane Piccin dos Santos ◽  
...  
Keyword(s):  
Renewable Energy ◽  
Microbial Community ◽  
Anaerobic Digestion ◽  
Biogas Production ◽  
Renewable Energy Sources ◽  
Starter Culture ◽  
Sequencing Analysis ◽  
Industrial Plants ◽  
Comprehensive Information ◽  
Culture Independent

Abstract The search for sustainable development has led countries around the world to seek the improvement of technologies that use renewable energy sources. One of the alternatives in the production of renewable energy comes from the use of waste including urban solids, animal excrement from livestock and biomass residues from agro-industrial plants. These materials may be used in the production of biogas, making its production highly sustainable and environmentally friendly, in addition to reducing public expenses for the treatment of those wastes. The present study evaluated the cultivated and uncultivated microbial community from a substrate (starter) used as an adapter for biogas production in anaerobic digestion processes. 16S rDNA metabarcoding revealed domain of bacteria belonging to the phyla Firmicutes, Bacteroidota, Chloroflexi and Synergistota. The methanogenic group was represented by the phyla Halobacterota and Euryarchaeota. Through 16S rRNA sequencing analysis of isolates recovered from the starter culture, the genera Rhodococcus, Vagococcus, Lysinibacillus, Niallia, Priestia, Robertmurraya, Luteimonas and Proteiniclasticum were recovered, groups that were not observed in the metabarcoding data. The groups mentioned are involved in the metabolism pathways of sugars and other compounds derived from lignocellulosic material, as well as in anaerobic methane production processes. The results demonstrate that culture-dependent approaches, such as isolation and sequencing of isolates, as well as culture-independent studies, such as the Metabarcoding approach, are complementary methodologies that, when integrated, provide robust and comprehensive information about the microbial communities involved in various processes, including the production of biogas in anaerobic digestion processes.

scholarly journals Adjusting Organic Load as a Strategy to Direct Single-Stage Food Waste Fermentation from Anaerobic Digestion to Chain Elongation

Processes ◽  
2020 ◽  
Vol 8 (11) ◽  
pp. 1487
Author(s):  
Vicky De Groof ◽  
Marta Coma ◽  
Tom C. Arnot ◽  
David J. Leak ◽  
Ana B. Lanham
Keyword(s):  
Anaerobic Digestion ◽  
Food Waste ◽  
Loading Rate ◽  
Biogas Production ◽  
Single Stage ◽  
Stirred Tank ◽  
Chain Elongation ◽  
Organic Loading Rate ◽  
Organic Loading ◽  
Operating Strategy

Production of medium chain carboxylic acids (MCCA) as renewable feedstock bio-chemicals, from food waste (FW), requires complicated reactor configurations and supplementation of chemicals to achieve product selectivity. This study evaluated the manipulation of organic loading rate in an un-supplemented, single stage stirred tank reactor to steer an anaerobic digestion (AD) microbiome towards acidogenic fermentation (AF), and thence to chain elongation. Increasing substrate availability by switching to a FW feedstock with a higher COD stimulated chain elongation. The MCCA species n-caproic (10.1 ± 1.7 g L−1) and n-caprylic (2.9 ± 0.8 g L−1) acid were produced at concentrations comparable to more complex reactor set-ups. As a result, of the adjusted operating strategy, a more specialised microbiome developed containing several MCCA-producing bacteria, lactic acid-producing Olsenella spp. and hydrogenotrophic methanogens. By contrast, in an AD reactor that was operated in parallel to produce biogas, the retention times had to be doubled when fed with the high-COD FW to maintain biogas production. The AD microbiome comprised a diverse mixture of hydrolytic and acidogenic bacteria, and acetoclastic methanogens. The results suggest that manipulation of organic loading rate and food-to-microorganism ratio may be used as an operating strategy to direct an AD microbiome towards AF, and to stimulate chain elongation in FW fermentation, using a simple, un-supplemented stirred tank set-up. This outcome provides the opportunity to repurpose existing AD assets operating on food waste for biogas production, to produce potentially higher value MCCA products, via simple manipulation of the feeding strategy.

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