Micro‐morphological identification study on Cordyceps sinensis (Berk.) Sacc. and its adulterants based on stereo microscope and desktop scanning electron microscope

Author(s):  
Shuai Kang ◽  
Li‐xing Nie ◽  
Yu‐guang Zheng ◽  
Tian‐tian Zuo ◽  
Ying Wang ◽  
...  
DEPIK ◽  
2014 ◽  
Vol 3 (2) ◽  
Author(s):  
Sulistiono . ◽  
Mujizat Kawaroe ◽  
Hawis Madduppa ◽  
Romanus Edy Prabowo

Abstract. Research on the sponge barnacle of Indonesia is very rare, and this study is aimed to describe the morphological characteristics of sponge barnacles and their specific relationship with their sponge host species. This research was a survey and sponge samples were collected by tearing apart any available sponge found in three sampling locations, typically coral reef areas of Weh Island, Seribu Islands, and Karimunjawa Islands. Sponge barnacles contained in sponge samples were observed using stereo microscope and scanning electron microscope, and species identification was determined based on the morphological description of Darwin (1854), Pilsbry (1916), Martin dan Davis (2001), and Kolbasov (1993). Four species of sponge barnacles were found consisting of Acasta cyathus, Acasta fenestrata, Euacasta dofleini, and Membranobalanus longirostrum. Specific relationships of barnacle and its sponge host were found between Euacasta dofleini and Haliclona sp. and between Membranobalanus longirostrum and Suberites sp. respectively. Keywords : sponge; barnacle; acasta; membranobalanus; biodiversityAbstrak. Penelitian teritip spons Indonesia jarang dilakukan sejak kelompok ini pertama kali dideskripsikan hingga saat ini. Tujuan dari penelitian ini adalah untuk mengkaji karakter morfologi teritip spons Indonesia dan untuk mengkaji hubungan spesies spesifik teritip terhadap spons. Pengambilan sampel pada penelitian ini dilaksanakan sejak Oktober sampai Desember 2012 di tiga lokasi yaitu Pulau Weh, Kepulauan Seribu, dan Kepulauan Karimunjawa. Metode penelitian menggunakan metode survei dan titik pengambilan sampel dipilih berdasarkan keberadaan spons. Sampel diamati menggunakan mikroskop stereo dan mikroskop elektron kemudian diidentifikasi berdasarkan deskripsi Darwin (1854), Pilsbry (1916), Martin dan Davis (2001), dan Kolbasov (1993). Hasil penelitian didapatkan empat spesies teritip spons yaitu Acasta cyathus, A. fenestrata, Euacasta dofleini, dan Membranobalanus longirostrum. Terdapat hubungan spesies spesifik antara teritip dan spons inang yaitu antara teritip E. dofleini dan spons Haliclona sp. dan antara teritip M. longirostrum dan spons Suberites sp. Kata kunci : teritip; spons; acasta; membranobalanus; biodiversitas


Author(s):  
Julio Parapar ◽  
Verónica Palomanes ◽  
Gudmundur V. Helgason ◽  
Juan Moreira

Based on samples collected during the BIOICE project off Iceland, four species of marine annelids belonging to the family Pectinariidae were identified: Amphictene auricoma (O.F. Müller, 1776), Cistenides granulata (Linnaeus, 1767), Cistenides hyperborea Malmgren, 1865 and Lagis koreni Malmgren, 1866. Taxonomic remarks and data on geographical and bathymetric distribution are presented. The distribution of each species off Iceland was evaluated and two patterns were defined: C. granulata and C. hyperborea were mainly found in waters off the northeast coast, while A. auricoma and L. koreni were found on the southern coast. Several body characters with taxonomic value in this family were reviewed under the stereo microscope and scanning electron microscope, with special emphasis on the neuropodial uncini. Remarks on these special chaetae are included in the diagnoses.


2018 ◽  
Vol 25 (2) ◽  
pp. 135-148
Author(s):  
Min-Jung Kong ◽  
Jun-Ho Song ◽  
Beom-Cheol An ◽  
Sung-Won Son ◽  
Gang-Uk Suh ◽  
...  

A comparative macro- and micromorphological study was conducted on achenes of Korean Polygonaceae comprising 45 taxa under 10 genera using a Stereo-microscope (SM) and Scanning Electron Microscope (SEM) to evaluate the taxonomical relevance of achene morphological characteristics. The achene shape is lenticular, biconvex, trigonous, or spheroidal. The largest achenes are found in Fagopyrum esculentum Moench (5.80–6.70 × 3.90–4.40 mm) and Rheum rhabarbarum L. (5.00–6.50 × 4.00–5.20 mm), and the smallest is found in Rumex maritimus L. (1.17–1.41 × 0.60–0.84 mm). Three types of embryo shape (curved, straight, and folded) and two types of embryo position (plane and parietal) can be distinguished. Most taxa have a curved embryo shape with plane position. Four types of surface patterns (smooth, papillae, tuberculate, or small pits) are observed. The surface sculpture or embryo type is rather consistent at the generic or tribal level, and the comprehensive consideration of achene characteristics is also useful at the species level. The achene morphology is described in detail, compared, and illustrated. The taxonomic significance of the achene morphology is discussed.


1995 ◽  
Vol 73 (11) ◽  
pp. 1842-1847 ◽  
Author(s):  
Christian R. Lacroix ◽  
Judith MacIntyre

This modification to the technique of epi-illumination light microscopy makes use of a new system of lenses that replaces expensive and not readily available dipping cone objectives. The newer objectives offer at least comparable resolution and depth of field, along with simple preparation procedures. An epi-illumination system is a good intermediate between the stereo microscope and a scanning electron microscope, offering magnification at high power that can aid in evaluation of potential scanning electron microscope specimens, as well as the time- and material-saving feature of being able to eliminate unsuitable scanning electron microscope specimens. Key words: technique, epi-illumination, morphogenesis, vegetative apex, primordium, staining.


2008 ◽  
Vol 55-57 ◽  
pp. 633-636
Author(s):  
D. Polsongkram ◽  
P. Kasian ◽  
Pichet Limsuwan ◽  
Udom Tipparach ◽  
S. Samran ◽  
...  

ZnO and other nanomaterials were synthesized by thermal evaporation process by carbon assisted method using powder ZnO as a precursors at temperature 1200 oC in one atmosphere of nitrogen for 3 hours. The diameter of nanofibers and nanowires vary from 50 nm to 200 nm and length of several ten micrometers. The size of nanorods vary from 20 nm to 100 nm and length of a few micrometers. The stereo microscope with an image analyzer and scanning electron microscope instruments are used to characterize these nanostructured materials.


Author(s):  
R. E. Ferrell ◽  
G. G. Paulson

The pore spaces in sandstones are the result of the original depositional fabric and the degree of post-depositional alteration that the rock has experienced. The largest pore volumes are present in coarse-grained, well-sorted materials with high sphericity. The chief mechanisms which alter the shape and size of the pores are precipitation of cementing agents and the dissolution of soluble components. Each process may operate alone or in combination with the other, or there may be several generations of cementation and solution.The scanning electron microscope has ‘been used in this study to reveal the morphology of the pore spaces in a variety of moderate porosity, orthoquartzites.


Author(s):  
C. T. Nightingale ◽  
S. E. Summers ◽  
T. P. Turnbull

The ease of operation of the scanning electron microscope has insured its wide application in medicine and industry. The micrographs are pictorial representations of surface topography obtained directly from the specimen. The need to replicate is eliminated. The great depth of field and the high resolving power provide far more information than light microscopy.


Author(s):  
K. Shibatomi ◽  
T. Yamanoto ◽  
H. Koike

In the observation of a thick specimen by means of a transmission electron microscope, the intensity of electrons passing through the objective lens aperture is greatly reduced. So that the image is almost invisible. In addition to this fact, it have been reported that a chromatic aberration causes the deterioration of the image contrast rather than that of the resolution. The scanning electron microscope is, however, capable of electrically amplifying the signal of the decreasing intensity, and also free from a chromatic aberration so that the deterioration of the image contrast due to the aberration can be prevented. The electrical improvement of the image quality can be carried out by using the fascionating features of the SEM, that is, the amplification of a weak in-put signal forming the image and the descriminating action of the heigh level signal of the background. This paper reports some of the experimental results about the thickness dependence of the observability and quality of the image in the case of the transmission SEM.


Author(s):  
S. Takashima ◽  
H. Hashimoto ◽  
S. Kimoto

The resolution of a conventional transmission electron microscope (TEM) deteriorates as the specimen thickness increases, because chromatic aberration of the objective lens is caused by the energy loss of electrons). In the case of a scanning electron microscope (SEM), chromatic aberration does not exist as the restrictive factor for the resolution of the transmitted electron image, for the SEM has no imageforming lens. It is not sure, however, that the equal resolution to the probe diameter can be obtained in the case of a thick specimen. To study the relation between the specimen thickness and the resolution of the trans-mitted electron image obtained by the SEM, the following experiment was carried out.


Author(s):  
R. F. Schneidmiller ◽  
W. F. Thrower ◽  
C. Ang

Solid state materials in the form of thin films have found increasing structural and electronic applications. Among the multitude of thin film deposition techniques, the radio frequency induced plasma sputtering has gained considerable utilization in recent years through advances in equipment design and process improvement, as well as the discovery of the versatility of the process to control film properties. In our laboratory we have used the scanning electron microscope extensively in the direct and indirect characterization of sputtered films for correlation with their physical and electrical properties.Scanning electron microscopy is a powerful tool for the examination of surfaces of solids and for the failure analysis of structural components and microelectronic devices.


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