Porphyrin induced structural destabilization of a parallel DNA G‐quadruplex in human MRP1 gene promoter

2022 ◽  
Author(s):  
Savita Joshi ◽  
Anju Singh ◽  
Shrikant Kukreti
Keyword(s):  
Gene Promoter ◽  
G Quadruplex

scholarly journals Up-Regulating Relaxin Expression by G-Quadruplex Interactive Ligand to Achieve Antifibrotic Action

Endocrinology ◽  
2012 ◽  
Vol 153 (8) ◽  
pp. 3692-3700 ◽  
Author(s):  
Hui-Ping Gu ◽  
Sen Lin ◽  
Ming Xu ◽  
Hai-Yi Yu ◽  
Xiao-Jun Du ◽  
...  
Keyword(s):  
Rna Polymerase Ii ◽  
Cardiac Fibrosis ◽  
Cardiac Fibroblasts ◽  
Heart Diseases ◽  
Gene Promoter ◽  
Binding Motif ◽  
Endogenous Expression ◽  
G Quadruplex

Myocardial fibrosis is a key pathological change in a variety of heart diseases contributing to the development of heart failure, arrhythmias, and sudden death. Recent studies have shown that relaxin prevents and reverses cardiac fibrosis. Endogenous expression of relaxin was elevated in the setting of heart disease; the extent of such up-regulation, however, is insufficient to exert compensatory actions, and the mechanism regulating relaxin expression is poorly defined. In the rat relaxin-1 (RLN1, Chr1) gene promoter region we found presence of repeated guanine (G)-rich sequences, which allowed formation and stabilization of G-quadruplexes with the addition of a G-quadruplex interactive ligand berberine. The G-rich sequences and the G-quadruplexes were localized adjacent to the binding motif of signal transducer and activator of transcription (STAT)3, which negatively regulates relaxin expression. Thus, we hypothesized that the formation and stabilization of G-quadruplexes by berberine could influence relaxin expression. We found that berberine-induced formation of G-quadruplexes did increase relaxin gene expression measured at mRNA and protein levels. Formation of G-quadruplexes significantly reduced STAT3 binding to the promoter of relaxin gene. This was associated with consequent increase in the binding of RNA polymerase II and STAT5a to relaxin gene promoter. In cardiac fibroblasts and rats treated with angiotensin II, berberine was found to suppress fibroblast activation, collagen synthesis, and extent of cardiac fibrosis through up-regulating relaxin. The antifibrotic action of berberine in vitro and in vivo was similar to that by exogenous relaxin. Our findings document a novel therapeutic strategy for fibrosis through up-regulating expression of endogenous relaxin.

scholarly journals Conformational Transition of a Hairpin Structure to G-Quadruplex within the WNT1 Gene Promoter

2014 ◽  
Vol 137 (1) ◽  
pp. 210-218 ◽  
Author(s):  
Margaret Hsin-Jui Kuo ◽  
Zi-Fu Wang ◽  
Ting-Yuan Tseng ◽  
Ming-Hao Li ◽  
Shang-Te Danny Hsu ◽  
...  
Keyword(s):  
Conformational Transition ◽  
Gene Promoter ◽  
Hairpin Structure ◽  
G Quadruplex

scholarly journals Oxidative Modification of the Potential G-Quadruplex Sequence in the PCNA Gene Promoter Can Turn on Transcription

2019 ◽  
Vol 32 (3) ◽  
pp. 437-446 ◽  
Author(s):  
Samuel C. J. Redstone ◽  
Aaron M. Fleming ◽  
Cynthia J. Burrows
Keyword(s):  
Gene Promoter ◽  
Oxidative Modification ◽  
Turn On ◽  
G Quadruplex

scholarly journals Assessment of gene promoter G-quadruplex binding and modulation by a naphthalene diimide derivative in tumor cells

2014 ◽  
Vol 46 (1) ◽  
pp. 369-380 ◽  
Author(s):  
MATTEO NADAI ◽  
GRAZIELLA CIMINO-REALE ◽  
GIOVANNA SATTIN ◽  
FILIPPO DORIA ◽  
ELENA BUTOVSKAYA ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Gene Promoter ◽  
Naphthalene Diimide ◽  
G Quadruplex

Superstructural self-assembly of the G-quadruplex structure formed by the homopurine strand in a DNA tract of human telomerase gene promoter

2008 ◽  
Vol 136 (2-3) ◽  
pp. 159-163 ◽  
Author(s):  
Sabrina Pisano ◽  
Michela Varra ◽  
Emanuela Micheli ◽  
Teresa Coppola ◽  
Pasquale De Santis ◽  
...  
Keyword(s):  
Self Assembly ◽  
Gene Promoter ◽  
Quadruplex Structure ◽  
G Quadruplex ◽  
Human Telomerase
Sign in / Sign up

Export Citation Format

Share Document