Genotyping of human papillomavirus in paraffin embedded cervical tissue samples from women in ethiopia and the Sudan

2012 ◽  
Vol 85 (2) ◽  
pp. 282-287 ◽  
Author(s):  
Ebba Abate ◽  
Abraham Aseffa ◽  
Muntasir El-Tayeb ◽  
Ibrahim El-Hassan ◽  
Lawrence Yamuah ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Cervical Tissue ◽  
Tissue Samples

scholarly journals Detection and Genotyping of Human Papillomavirus in Cervical Tissue Samples in Ahvaz, Southwest Iran

2012 ◽  
Vol 6 (7) ◽  
Author(s):  
Mojtaba Hamidi-Fard ◽  
Mojtaba Fattahi-Abdizadeh ◽  
Manoochehr Makvandi ◽  
Nastaran Ranjbari ◽  
Esrafil Mansoori ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Cervical Tissue ◽  
Tissue Samples ◽  
Southwest Iran

The interaction between steroid hormones, human papillomavirus type 16, E6 oncogene expression, and cervical cancer

2003 ◽  
Vol 13 (6) ◽  
pp. 834-842 ◽  
Author(s):  
M. Moodley ◽  
S. Sewart ◽  
C. S. Herrington ◽  
R. Chetty ◽  
R. Pegoraro ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Steroid Hormones ◽  
Housekeeping Gene ◽  
Hpv 16 ◽  
Tissue Samples ◽  
Hpv Dna ◽  
Human Papillomavirus Type 16 ◽  
Transforming Proteins

Various risk factors have been implicated in the causation of cervical cancer including human papillomavirus (HPV), the early genes (E6 and E7) of which encode the main transforming proteins. Studies have suggested that steroid hormones may enhance the expression of these genes leading to loss of p53 gene-mediated cell apoptosis. A total of 120 cervical tissue samples were obtained from patients with proven cervical cancer. Patients who used depo-medroxyprogesterone acetate steroid contraception were recruited as part of the steroid arm. Only HPV DNA type 16 samples were used for the study. Controls included three cell lines (CaSki, SiHa, & C33A) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an internal housekeeping gene. Of 120 patients, there were 111 patients with HPV type 16 identified. Of this number, RNA was present in 63 samples. There were 30 women (30/63) who used steroid contraception. In relation to patients who used contraception, HPV 16 E6 gene expression was present in 79% (n = 23) and 88% (n = 30) of steroid users compared to nonusers, respectively. In total there were 25 patients (40%) with expression of the HPV 16 E6*I gene and 30 patients with expression of the E6*II gene. There were 57% of steroid users (n = 17) who had expression of the E6*I/E6*II gene, compared to 52% (n = 17) of nonusers (P = 0.800). From a molecular level, this study does not confirm the role of injectable progesterones in cervical carcinogenesis.

High-risk Human Papillomavirus Determination in Formalin-fixed, Paraffin-embedded Cervical Tissue Using the Roche Cobas 4800 System

2019 ◽  
Vol 38 (1) ◽  
pp. 11-16 ◽  
Author(s):  
Juan C. Tardío ◽  
Olivia Cambero ◽  
Carolina Sánchez-Estévez ◽  
Ana B. Sánchez-García ◽  
Fernando Angulo ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Cervical Tissue ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed ◽  
Roche Cobas

Squamous Intraepithelial Lesions in Cervical Tissue Samples of Limited Adequacy and Insufficient for Grading as Low or High Grade

2015 ◽  
Vol 19 (1) ◽  
pp. 35-45 ◽  
Author(s):  
Sandra Lee ◽  
Jeanelle Sabourin ◽  
Julia Gage ◽  
Angela Franko ◽  
Jill G. Nation ◽  
...  
Keyword(s):  
High Grade ◽  
Cervical Tissue ◽  
Squamous Intraepithelial Lesions ◽  
Tissue Samples ◽  
Intraepithelial Lesions

Ras and C-Myc Oncoproteins during Tumor Progression in the Uterine Cervix

1998 ◽  
Vol 84 (5) ◽  
pp. 583-588 ◽  
Author(s):  
Sivakumari Asha Nair ◽  
Madhavan Balaraman Nair ◽  
Puthuveetil Govindan Jayaprakash ◽  
Thayyullathil Nellika Rajalekshmy ◽  
Madhavan Krishnan Nair ◽  
...  
Keyword(s):  
Tumor Progression ◽  
Uterine Cervix ◽  
Mean Value ◽  
Low Grade ◽  
Cervical Tissue ◽  
Ras Gene ◽  
Tissue Samples ◽  
Cervical Epithelium ◽  
Malignant Lesions ◽  
The Many

Aims and background Altered oncogenic activity is a feature associated with many malignant and premalignant conditions. Among the many oncogenes, ras and myc are commonly altered in many tumors. This study aims to evaluate the expression of ras and c-myc oncoproteins in a total of 204 cervical tissue samples, including premalignant and malignant lesions as well as apparently normal cervical tissue. Methods and study design Mouse monoclonal antibodies against the three mammalian ras gene products (c-H-ras, c-K-ras, c-N-ras) and the c-myc protein were used to evaluate oncoprotein expression by immunocytochemistry. Results None of the samples analyzed displayed immunoreactivity for H-ras and K-ras. Normal cervical epithelium showed minimal immunoreactivity for N-ras with about 33% of the samples expressing the protein. More conspicuous expression in normal tissue was displayed by c-myc, with about 90% of the samples expressing the protein (mean value of cells positive = 34%). The immunoreactivity for N-ras increased with increasing histological abnormality from low-grade squamous intraepithelial lesions (SIL) to invasive carcinoma. Increased immunoreactivity for N-ras was evident in the basaloid cells of malignant lesions, with the maximum value of 66% found in poorly differentiated squamous cell carcinoma (PDSCC). The percentage of nuclei positive for c-myc also showed a gradual increase from low-grade SIL onwards, the highest positivity being found in PDSCC, where the mean value was 85%. Statistical analysis revealed a good correlation between the expression of N-ras (r = 0.8922, P = 0.001) and c-myc (r = 0.8856, P =0.001) and various histological stages of tumor progression in the cervical epithelium. Conclusions These results therefore suggest that c-myc and N-ras oncoproteins are important during tumor progression in the uterine cervix.

Prevalence and genotype distribution of human papillomavirus in non-neoplastic cervical tissue lesion: Cervical erosion

2011 ◽  
Vol 83 (11) ◽  
pp. 1997-2003 ◽  
Author(s):  
Aysen Bayram ◽  
Suna Erkılıç ◽  
Özcan Balat ◽  
Fahriye Ekşi ◽  
Mete Gürol Uğur ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Genotype Distribution ◽  
Cervical Tissue ◽  
Tissue Lesion

scholarly journals Genome-Wide Profiling of Cervical RNA-Binding Proteins Identifies Human Papillomavirus Regulation of RNASEH2A Expression by Viral E7 and E2F1

mBio ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. e02687-18 ◽  
Author(s):  
Junfen Xu ◽  
Habin Liu ◽  
Yanqin Yang ◽  
Xiaohong Wang ◽  
Poching Liu ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Binding Proteins ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Cancer Tissue ◽  
Cervical Tissue ◽  
Tissue Samples ◽  
Altered Expression ◽  
Cervical Cancer Tissue ◽  
E6 And E7

ABSTRACTRNA-binding proteins (RBPs) control mRNA processing, stability, transport, editing, and translation. We recently conducted transcriptome analyses comparing normal (i.e., healthy) cervical tissue samples with human papillomavirus (HPV)-positive cervical cancer tissue samples and identified 614 differentially expressed protein-coding transcripts which are enriched in cancer-related pathways and consist of 95 known RBPs. We verified the altered expression of 26 genes with a cohort of 72 cervical samples, including 24 normal cervical samples, 25 cervical intraepithelial neoplasia grade 2 (CIN2) and CIN3 samples, and 23 cervical cancer tissue samples. LY6K (lymphocyte antigen 6 complex locus K), FAM83A (family member with sequence similarity 83), CELSR3, ASF1B, IQGAP3, SEMA3F, CLDN10, MSX1, CXCL5, ASRGL1, ELAVL2, GRB7, KHSRP, NOVA1, PTBP1, and RNASEH2A were identified as novel candidate genes associated with cervical lesion progression and carcinogenesis. HPV16 or HPV18 infection was found to alter the expression of 8 RBP genes (CDKN2A, ELAVL2, GRB7, HSPB1, KHSRP, NOVA1, PTBP1, and RNASEH2A) in human vaginal and foreskin keratinocytes. Both viral E6 and E7 decreased NOVA1 expression, but only E7 increased the expression of RNASEH2A in an E2F1-dependent manner. Proliferating cell nuclear antigen (PCNA) directs RNASEH2 activity with respect to DNA replication by removing the RNA primers to promote Okazaki fragment maturation, and two factors are closely associated with neoplasia progression. Therefore, we predict that the induction of expression of RNASEH2A via viral E7 and E2F1 may promote DNA replication and cancer cell proliferation.IMPORTANCEHigh-risk HPV infections lead to development of cervical cancer. This study identified the differential expression of 16 novel genes (LY6K, FAM83A, CELSR3, ASF1B, IQGAP3, SEMA3F, CLDN10, MSX1, CXCL5, ASRGL1, ELAVL2, GRB7, KHSRP, NOVA1, PTBP1, and RNASEH2A) in HPV-infected cervical tissue samples and keratinocytes. Eight of these genes (CDKN2A, ELAVL2, GRB7, HSPB1, KHSRP, NOVA1, PTBP1, and RNASEH2A) encode RNA-binding proteins. Further studies indicated that both HPV16 and HPV18 infections lead to the aberrant expression of selected RBP-encoding genes. We found that viral E6 and E7 decrease NOVA1 expression but that E7 increases RNASEH2A expression via E2F1. The altered expression of these genes may be utilized as biomarkers for high-risk (HR)-HPV carcinogenesis and progression.

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