Neuropeptide Y modulates membrane excitability in neonatal rat mesencephalic V neurons

2020 ◽  
Vol 98 (5) ◽  
pp. 921-935
Author(s):  
Soju Seki ◽  
Susumu Tanaka ◽  
Saori Yamada ◽  
Tadataka Tsuji ◽  
Akifumi Enomoto ◽  
...  
Keyword(s):  
Neuropeptide Y ◽  
Neonatal Rat ◽  
Membrane Excitability

Neuropeptide Y damages the integrity of mitochondrial structure and disrupts energy metabolism in cultured neonatal rat cardiomyocytes

Peptides ◽  
2015 ◽  
Vol 71 ◽  
pp. 162-169 ◽  
Author(s):  
Guochang Luo ◽  
Xuehua Xu ◽  
Wei Guo ◽  
Chengliang Luo ◽  
Hao Wang ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Neuropeptide Y ◽  
Neonatal Rat ◽  
Rat Cardiomyocytes ◽  
Neonatal Rat Cardiomyocytes ◽  
Mitochondrial Structure

Neuropeptide Y Induces Cardiomyocyte Hypertrophy via Attenuating miR-29a-3p in Neonatal Rat Cardiomyocytes

2020 ◽  
Vol 27 (9) ◽  
pp. 878-887
Author(s):  
Yuxin Xie ◽  
Jun Hu ◽  
Xincai Zhang ◽  
Chunxiao Li ◽  
Yuanyi Zuo ◽  
...  
Keyword(s):  
Neuropeptide Y ◽  
Mitochondrial Dynamics ◽  
Treatment Plan ◽  
Neonatal Rat ◽  
Cardiomyocyte Hypertrophy ◽  
Down Regulation ◽  
Rat Cardiomyocytes ◽  
Expression Levels ◽  
Cell Energy ◽  
Potential Binding Site

Background: Neuropeptide Y (NPY) has been well known to induce cardiomyocyte hypertrophy (CH), which is possibly caused by disruption of cardiac cell energy balance. As mitochondria is losely related to energy metabolism, in this study, we investigated the changes in mitochondrial dynamics-related protein (Drp1) expression under the action of NPY. miRNA-29a, a endogenous noncoding small molecule RNA which is involved in many cardiac diseases, by using a bioinformatics tool, we found a potential binding site of miRNA-29a on the Drp1 mRNA, and suggesting that miRNA-29a might play a regulatory role. Methods: Objective: To investigate the role of miR-29a-3p in the process of NPY-induced CH, and further explore it’s predicted relationship with Drp1. The expression levels of miR-29a-3p and atrial natriuretic peptide (ANP) were performed by the method of fluorescence quantitative PCR, in addition, expression of Drp1 in treated and control groups were performed by western blot analysis. Results: We found NPY leads to the CH and up-regulation of ANP expression levels. We also found significant up-regulation of Drp1 expression and down-regulation of miR-29a-3p expression in NPYtreated cells. The decrease in miR-29a-3p expression may lead the increase expression level of Drp1. We found that the expression of ANP increased after NPY treatment. When Drp1 protein was silenced, the high expression of ANP was inhibited. Conclusion: In this study, we found up-regulation of Drp1 in cells treated with NPY. Drp1 mRNA is a predicted target for miR-29a-3p, and the expression of Drp1 was attenuated by miR-29a-3p. Therefore, NPY leads to down-regulation of miR-29a-3p expression, up-regulation of Drp1 expression, and NPY leads to CH. Correspondingly, miR-29a-3p can counteract the effects of NPY. This may be a new way, which could be used in diagnosis and treatment plan for CH.

Identification of neuropeptide Y receptors in cultured astrocytes from neonatal rat brain

1993 ◽  
Vol 34 (2) ◽  
pp. 198-205 ◽  
Author(s):  
G. Gimpl ◽  
F. Kirchhoff ◽  
R. E. Lang ◽  
Helmut Kettenmann
Keyword(s):  
Neuropeptide Y ◽  
Rat Brain ◽  
Neonatal Rat ◽  
Cultured Astrocytes ◽  
Neonatal Rat Brain ◽  
Y Receptors ◽  
Neuropeptide Y Receptors

Cytochemical Evidence for Presynatic β-Adrenergic Regulation of Secretion in the Developing Rat Submandibular Gland

1977 ◽  
Vol 35 ◽  
pp. 430-431
Author(s):  
L.S. Cutler
Keyword(s):  
Cyclic Amp ◽  
Adenylate Cyclase ◽  
Submandibular Gland ◽  
Neonatal Rat ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Parotid Glands ◽  
Adrenergic Agonist ◽  
Rat Submandibular Gland

Many studies previously have shown that the B-adrenergic agonist isoproterenol and the a-adrenergic agonist norepinephrine will stimulate secretion by the adult rat submandibular (SMG) and parotid glands. Recent data from several laboratories indicates that adrenergic agonists bind to specific receptors on the secretory cell surface and stimulate membrane associated adenylate cyclase activity which generates cyclic AMP. The production of cyclic AMP apparently initiates a cascade of events which culminates in exocytosis. During recent studies in our laboratory it was observed that the adenylate cyclase activity in plasma membrane fractions derived from the prenatal and early neonatal rat submandibular gland was retractile to stimulation by isoproterenol but was stimulated by norepinephrine. In addition, in vitro secretion studies indicated that these prenatal and neonatal glands would not secrete peroxidase in response to isoproterenol but would secrete in response to norepinephrine. In contrast to these in vitro observations, it has been shown that the injection of isoproterenol into the living newborn rat results in secretion of peroxidase by the SMG (1).

Abnormal levels of neuropeptide Y and peptide YY in colon in the irritable bowel syndrome (IBS)

2001 ◽  
Vol 120 (5) ◽  
pp. A753-A754
Author(s):  
M SIMREN ◽  
G RINGSTROM ◽  
P STOTZER ◽  
H ABRAHAMSSON ◽  
E BJOMSSON
Keyword(s):  
Irritable Bowel Syndrome ◽  
Neuropeptide Y ◽  
Peptide Yy ◽  
Irritable Bowel

Repeated isolation stress in the neonatal rat: Relation to brain dopamine systems in the 10-day-old rat.

1998 ◽  
Vol 112 (6) ◽  
pp. 1466-1474 ◽  
Author(s):  
Priscilla Kehoe ◽  
W. J. Shoemaker ◽  
C. Arons ◽  
L. Triano ◽  
G. Suresh
Keyword(s):  
Neonatal Rat ◽  
Brain Dopamine ◽  
Isolation Stress

Low cerebrospinal fluid neuropeptide Y concentrations in posttraumatic stress disorder

2009 ◽  
Author(s):  
R. Sah ◽  
N. N. Ekhator ◽  
J. R. Strawn ◽  
F. R. Sallee ◽  
D. G. Baker ◽  
...  
Keyword(s):  
Posttraumatic Stress Disorder ◽  
Cerebrospinal Fluid ◽  
Neuropeptide Y ◽  
Posttraumatic Stress ◽  
Stress Disorder
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