Isolation of a plasma membrane-enriched fraction from collagenase-suspended rachitic rat growth plate chondrocytes

1983 ◽  
Vol 1 (3) ◽  
pp. 319-324 ◽  
Author(s):  
William W. Bohn ◽  
Ross M. Stein ◽  
Howard H. T. Hsu ◽  
David C. Morris ◽  
H. Clarke Anderson
Keyword(s):  
Plasma Membrane ◽  
Growth Plate ◽  
Growth Plate Chondrocytes ◽  
Rat Growth

scholarly journals Valproic Acid Impacts the Growth of Growth Plate Chondrocytes

2020 ◽  
Vol 17 (10) ◽  
pp. 3675
Author(s):  
Hueng-Chuen Fan ◽  
Shih-Yu Wang ◽  
Yi-Jen Peng ◽  
Herng-Sheng Lee
Keyword(s):  
Valproic Acid ◽  
Short Stature ◽  
Growth Plate ◽  
Caspase 3 ◽  
Skeletal Growth ◽  
Growth Plate Chondrocytes ◽  
Protein Levels ◽  
Rat Growth ◽  
Cox 2 ◽  
Bone Abnormalities

A range of bone abnormalities including short stature have been reported to be associated with the use of antiepileptic drugs (AEDs) in children. Exactly how AEDs impact skeletal growth, however, is not clear. In the present study, rat growth plate chondrocytes were cultured to study the effects of AEDs, including valproic acid (VPA), oxcarbazepine (OXA), levetiracetam (LEV), lamotrigine (LTG), and topiramate (TPM) on the skeletal growth. VPA markedly reduced the number of chondrocytes by apoptosiswhile other AEDs had no effect. The apoptosis associated noncleaved and cleaved caspase 3, and caspases were increased by exposure to VPA, which up-regulated cyclooxygenase 2 (COX-2) mRNA and protein levels likely through histone acetylation. The COX-2 inhibitor NS-398 attenuated the effects of VPA up-regulating COX-2 expression and decreased VPA-induced caspase 3 expression. The use of VPA in children should be closely monitored or replaced, where appropriate, by AEDs which do not apparently affect the growth plate chondrocytes.

Autoradiographic Evidence of Opioid Binding Sites in Rat Growth Plate Chondrocytes

1992 ◽  
Vol 55 (3) ◽  
pp. 357-359 ◽  
Author(s):  
Manuel Freire-Garabal ◽  
Maria Teresa Castaño ◽  
Angel Belmonte ◽  
Javier Jorge ◽  
José Couceiro ◽  
...  
Keyword(s):  
Growth Plate ◽  
Binding Sites ◽  
Growth Plate Chondrocytes ◽  
Opioid Binding ◽  
Rat Growth

Rat growth plate chondrocytes express low levels of 25-hydroxy-1α-hydroxylase

2004 ◽  
Vol 89-90 ◽  
pp. 143-147 ◽  
Author(s):  
Ulrike Hügel ◽  
Lutz Weber ◽  
Jörg Reichrath ◽  
Otto Mehls ◽  
Günter Klaus
Keyword(s):  
Growth Plate ◽  
Growth Plate Chondrocytes ◽  
Rat Growth ◽  
Low Levels

scholarly journals Interaction of IGF-I and 1α,25(OH)2D3 on receptor expression and growth stimulation in rat growth plate chondrocytes

1998 ◽  
Vol 53 (5) ◽  
pp. 1152-1161 ◽  
Author(s):  
Günter Klaus ◽  
Lutz Weber ◽  
Julian Rodríguez ◽  
Porfirio Fernández ◽  
Thomas Klein ◽  
...  
Keyword(s):  
Growth Plate ◽  
Growth Stimulation ◽  
Receptor Expression ◽  
Growth Plate Chondrocytes ◽  
Igf I ◽  
Rat Growth

Primary culture of rat growth plate chondrocytes: an in vitro model of growth plate histotype, matrix vesicle biogenesis and mineralization

Bone ◽  
2004 ◽  
Vol 34 (6) ◽  
pp. 961-970 ◽  
Author(s):  
Rama Garimella ◽  
Xiahong Bi ◽  
Nancy Camacho ◽  
Joseph B Sipe ◽  
H.Clarke Anderson
Keyword(s):  
Primary Culture ◽  
Growth Plate ◽  
In Vitro Model ◽  
Matrix Vesicle ◽  
Growth Plate Chondrocytes ◽  
Vesicle Biogenesis ◽  
Rat Growth ◽  
Vitro Model

Static Compressive Loading Reduces the mRNA Expression of Type II and X Collagen in Rat Growth-Plate Chondrocytes During Postnatal Growth

2005 ◽  
Vol 46 (4-5) ◽  
pp. 211-219 ◽  
Author(s):  
I. Villemure ◽  
M. A. Chung ◽  
C. S. Seck ◽  
M. H. Kimm ◽  
J. R. Matyas ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Growth Plate ◽  
Compressive Loading ◽  
Postnatal Growth ◽  
Type Ii ◽  
Growth Plate Chondrocytes ◽  
Rat Growth

scholarly journals Defined Carboxy-Terminal Fragments of Insulin-Like Growth Factor (IGF) Binding Protein-2 Exert Similar Mitogenic Activity on Cultured Rat Growth Plate Chondrocytes as IGF-I

Endocrinology ◽  
2008 ◽  
Vol 149 (10) ◽  
pp. 4901-4911 ◽  
Author(s):  
Daniela Kiepe ◽  
Anke Van Der Pas ◽  
Sonia Ciarmatori ◽  
Ludger Ständker ◽  
Burkhardt Schütt ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Biological Activity ◽  
Growth Plate ◽  
Longitudinal Growth ◽  
Growth Plate Chondrocytes ◽  
Igf I ◽  
Rat Growth ◽  
Igf Binding ◽  
Carboxy Terminal ◽  
Igf Binding Protein

The IGF/IGF binding protein (IGFBP) system is an important component in the hormonal regulation of longitudinal growth. Evidence from in vitro studies indicates that IGFBPs may have IGF-independent effects. We analyzed the biological activity of intact IGFBP-2 and defined carboxy-terminal IGFBP-2 fragments isolated from human hemofiltrate in two cell culture systems of the growth plate: rat growth plate chondrocytes in primary culture and the mesenchymal chondrogenic cell line RCJ3.1C5.18. The IGFBP-2 fragments IGFBP-2167–279, IGFBP-2167–289, and IGFBP-2104–289 exerted a strong (2- to 3-fold) mitogenic effect on growth plate chondrocytes, which was comparable with IGF-I in equimolar concentrations (7.8 nm) but was not mediated through the type 1 IGF receptor. In a dose-response experiment, the most effective concentration of IGFBP-2104–289 for the stimulation of cell proliferation was 10 nm. This biological activity of IGFBP-2 fragments was associated with cell membrane binding, demonstrated by Western blot analysis of fractionated cell lysates and immunohistochemistry. Whereas intact IGFBP-2 did not modulate chondrocyte proliferation, partially reduced (by dithiothreitol) full-length IGFBP-2 stimulated cell proliferation to a comparable extent (3.4-fold) as carboxy-terminal IGFBP-2 fragments. The mitogenic activity of these IGFBP-2 fragments and of partially reduced full-length IGFBP-2 was mediated through the use of the MAPK/ERK 1/2. These data imply a novel role of naturally occurring IGFBP-2 fragments for the endocrine and paracrine/autocrine regulation of longitudinal growth.

Sign in / Sign up

Export Citation Format

Share Document