scholarly journals Surgical preparation of bone-scaffold interface is critical for bone regeneration inside tissue engineering scaffold

2010 ◽  
Vol 29 (5) ◽  
pp. 767-772 ◽  
Author(s):  
Alireza Roshan-Ghias ◽  
Dominique P. Pioletti
Keyword(s):  
Tissue Engineering ◽  
Bone Regeneration ◽  
Tissue Engineering Scaffold ◽  
Bone Scaffold ◽  
Surgical Preparation

scholarly journals An in situ tissue engineering scaffold with growth factors combining angiogenesis and osteoimmunomodulatory functions for advanced periodontal bone regeneration

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Tian Ding ◽  
Wenyan Kang ◽  
Jianhua Li ◽  
Lu Yu ◽  
Shaohua Ge
Keyword(s):  
Tissue Engineering ◽  
Growth Factors ◽  
Bone Regeneration ◽  
Bone Defect ◽  
Bone Repair ◽  
Macrophage Polarization ◽  
Tissue Engineering Scaffold ◽  
Bone Morphogenetic Protein 2 ◽  
In Situ Tissue Engineering

Abstract Background The regeneration of periodontal bone defect remains a vital clinical challenge. To date, numerous biomaterials have been applied in this field. However, the immune response and vascularity in defect areas may be key factors that are overlooked when assessing the bone regeneration outcomes of biomaterials. Among various regenerative therapies, the up-to-date strategy of in situ tissue engineering stands out, which combined scaffold with specific growth factors that could mimic endogenous regenerative processes. Results Herein, we fabricated a core/shell fibrous scaffold releasing basic fibroblast growth factor (bFGF) and bone morphogenetic protein-2 (BMP-2) in a sequential manner and investigated its immunomodulatory and angiogenic properties during periodontal bone defect restoration. The in situ tissue engineering scaffold (iTE-scaffold) effectively promoted the angiogenesis of periodontal ligament stem cells (PDLSCs) and induced macrophage polarization into pro-healing M2 phenotype to modulate inflammation. The immunomodulatory effect of macrophages could further promote osteogenic differentiation of PDLSCs in vitro. After being implanted into the periodontal bone defect model, the iTE-scaffold presented an anti-inflammatory response, provided adequate blood supply, and eventually facilitated satisfactory periodontal bone regeneration. Conclusions Our results suggested that the iTE-scaffold exerted admirable effects on periodontal bone repair by modulating osteoimmune environment and angiogenic activity. This multifunctional scaffold holds considerable promise for periodontal regenerative medicine and offers guidance on designing functional biomaterials. Graphic Abstract

Progress in polymer nanocomposites for bone regeneration and engineering

2020 ◽  
pp. 096739112091365 ◽  
Author(s):  
Christopher Igwe Idumah
Keyword(s):  
Tissue Engineering ◽  
Mechanical Behavior ◽  
Bone Regeneration ◽  
Polymer Nanocomposites ◽  
Metallic Nanoparticles ◽  
Bone Repair ◽  
Fiber Composites ◽  
Bone Scaffold ◽  
Medical Field ◽  
The Matrix

The ultimate aim of tissue engineering entails fabrication of functional replacements for damaged organs or tissues. Scaffolds facilitate the proliferation of cells, while also improving their various functions. Scaffolds are 3-D structures capable of imitating mechanical and bioactive behaviors of tissues extracellular matrix, which provides enabling environment for cellular bonding, proliferation, and distinction. Hence, scaffolds are often applied in tissue engineering with the aim of facilitating damaged tissue regeneration which is a very important aspect of bone repair. Polymers are broadly utilized in tissue engineering due to their inherent versatility. However, polymers cannot attain mechanical behavior comparable to the bone. Thus, polymer nanocomposites fabricated through inclusion of fibers/or uniformly distributed ceramic/metallic nanoparticles in the matrix are potential materials for bone scaffold fabrication because inclusion of fiber or nanoparticles enhances composites mechanical behavior, while also improving other properties. Hence, this article elucidates recent trailblazing studies in polymer fiber composites and nanocomposites applied in the medical field especially in tissue engineering and bone regeneration. Also insights into market prospects and forecasts are presented.

Hierarchical micro/submicrometer-scale structured scaffolds preparedviacoaxial electrospinning for bone regeneration

2017 ◽  
Vol 5 (46) ◽  
pp. 9219-9228 ◽  
Author(s):  
Chen Tao ◽  
Yanxia Zhang ◽  
Bin Li ◽  
Liang Chen
Keyword(s):  
Tissue Engineering ◽  
Bone Regeneration ◽  
Tissue Engineering Scaffold ◽  
Coaxial Electrospinning

A tissue engineering scaffold based on hierarchical micro/submicrometer-scale structured core–sheath fibers is preparedviacoaxial electrospinning for bone regeneration.

Decellularized bone extracellular matrix in skeletal tissue engineering

2020 ◽  
Vol 48 (3) ◽  
pp. 755-764
Author(s):  
Benjamin B. Rothrauff ◽  
Rocky S. Tuan
Keyword(s):  
Tissue Engineering ◽  
Bone Regeneration ◽  
Tissue Regeneration ◽  
Animal Studies ◽  
Tumor Resection ◽  
Regenerative Capacity ◽  
Skeletal Tissue ◽  
Large Bone

Bone possesses an intrinsic regenerative capacity, which can be compromised by aging, disease, trauma, and iatrogenesis (e.g. tumor resection, pharmacological). At present, autografts and allografts are the principal biological treatments available to replace large bone segments, but both entail several limitations that reduce wider use and consistent success. The use of decellularized extracellular matrices (ECM), often derived from xenogeneic sources, has been shown to favorably influence the immune response to injury and promote site-appropriate tissue regeneration. Decellularized bone ECM (dbECM), utilized in several forms — whole organ, particles, hydrogels — has shown promise in both in vitro and in vivo animal studies to promote osteogenic differentiation of stem/progenitor cells and enhance bone regeneration. However, dbECM has yet to be investigated in clinical studies, which are needed to determine the relative efficacy of this emerging biomaterial as compared with established treatments. This mini-review highlights the recent exploration of dbECM as a biomaterial for skeletal tissue engineering and considers modifications on its future use to more consistently promote bone regeneration.

Tissue-Engineered Hydroxyapatite Bone Scaffold Impregnated with Osteoprogenitor Cells Promotes Bone Regeneration in Sheep Model

2021 ◽  
Author(s):  
Mohd Yazid Bajuri ◽  
Nanchappan Selvanathan ◽  
Fatin Nadira Dzeidee Schaff ◽  
Muhammad Haziq Abdul Suki ◽  
Angela Min Hwei Ng
Keyword(s):  
Bone Regeneration ◽  
Bone Scaffold ◽  
Sheep Model ◽  
Osteoprogenitor Cells

scholarly journals Sinusoidal electromagnetic fields accelerate bone regeneration by boosting the multifunctionality of bone marrow mesenchymal stem cells

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Weigang Li ◽  
Wenbin Liu ◽  
Wei Wang ◽  
Jiachen Wang ◽  
Tian Ma ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Bone Regeneration ◽  
Electromagnetic Fields ◽  
Bone Defects ◽  
Cell Scaffolds ◽  
Marrow Mesenchymal Stem Cells ◽  
Calvarial Defects

Abstract Background The repair of critical-sized bone defects is always a challenging problem. Electromagnetic fields (EMFs), used as a physiotherapy for bone defects, have been suspected to cause potential hazards to human health due to the long-term exposure. To optimize the application of EMF while avoiding its adverse effects, a combination of EMF and tissue engineering techniques is critical. Furthermore, a deeper understanding of the mechanism of action of EMF will lead to better applications in the future. Methods In this research, bone marrow mesenchymal stem cells (BMSCs) seeded on 3D-printed scaffolds were treated with sinusoidal EMFs in vitro. Then, 5.5 mm critical-sized calvarial defects were created in rats, and the cell scaffolds were implanted into the defects. In addition, the molecular and cellular mechanisms by which EMFs regulate BMSCs were explored with various approaches to gain deeper insight into the effects of EMFs. Results The cell scaffolds treated with EMF successfully accelerated the repair of critical-sized calvarial defects. Further studies revealed that EMF could not directly induce the differentiation of BMSCs but improved the sensitivity of BMSCs to BMP signals by upregulating the quantity of specific BMP (bone morphogenetic protein) receptors. Once these receptors receive BMP signals from the surrounding milieu, a cascade of reactions is initiated to promote osteogenic differentiation via the BMP/Smad signalling pathway. Moreover, the cytokines secreted by BMSCs treated with EMF can better facilitate angiogenesis and osteoimmunomodulation which play fundamental roles in bone regeneration. Conclusion In summary, EMF can promote the osteogenic potential of BMSCs and enhance the paracrine function of BMSCs to facilitate bone regeneration. These findings highlight the profound impact of EMF on tissue engineering and provide a new strategy for the clinical treatment of bone defects.

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