scholarly journals Reduced tonicity stimulates an inflammatory response in nucleus pulposus tissue that can be limited by a COX‐2‐specific inhibitor

2015 ◽  
Vol 33 (11) ◽  
pp. 1724-1731 ◽  
Author(s):  
Bart van Dijk ◽  
Esther Potier ◽  
Maarten van DIjk ◽  
Marloes Langelaan ◽  
Nicole Papen‐Botterhuis ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Nucleus Pulposus ◽  
Specific Inhibitor ◽  
Nucleus Pulposus Tissue ◽  
Cox 2

Significant reduction in serious upper gastrointestinal (UGI) events with celecoxib, a COX-2 specific inhibitor, compared with conventional NSAIDs. The SUCCESS I trial

2001 ◽  
Vol 120 (5) ◽  
pp. A105-A105
Author(s):  
J GOLDSTEIN ◽  
G EISEN ◽  
W STENSON ◽  
N AGRAWAL ◽  
A BELLO ◽  
...  
Keyword(s):  
Specific Inhibitor ◽  
Cox 2 ◽  
Upper Gastrointestinal

Sorghum Fermented by Aspergillus oryzae NK Enhances Inhibition of Vascular Inflammation in TNF-α-stimulated Human Aortic Smooth Muscle Cells

2018 ◽  
Vol 88 (5-6) ◽  
pp. 309-318
Author(s):  
Hae Seong Song ◽  
Jung-Eun Kwon ◽  
Hyun Jin Baek ◽  
Chang Won Kim ◽  
Hyelin Jeon ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Inflammatory Response ◽  
Smooth Muscle Cells ◽  
Adhesion Molecule ◽  
Vascular Inflammation ◽  
Muscle Cells ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle ◽  
Tnf Α ◽  
Cox 2

Abstract. Sorghum bicolor L. Moench is widely grown all over the world for food and feed. The effects of sorghum extracts on general inflammation have been previously studied, but its anti-vascular inflammatory effects are unknown. Therefore, this study investigated the anti-vascular inflammation effects of sorghum extract (SBE) and fermented extract of sorghum (fSBE) on human aortic smooth muscle cells (HASMCs). After the cytotoxicity test of the sorghum extract, a series of experiments were conducted. The inhibition effects of SBE and fSBE on the inflammatory response and adhesion molecule expression were measured using treatment with tumor necrosis factor-α (TNF-α), a crucial promoter for the development of atherosclerotic lesions, on HASMCs. After TNF-α (10 ng/mL) treatment for 2 h, then SBE and fSBE (100 and 200 μg/mL) were applied for 12h. Western blotting analysis showed that the expression of vascular cell adhesion molecule-1 (VCAM-1) (2.4-fold) and cyclooxygenase-2 (COX-2) (6.7-fold) decreased, and heme oxygenase-1 (HO-1) (3.5-fold) increased compared to the TNF-α control when treated with 200 μg/mL fSBE (P<0.05). In addition, the fSBE significantly increased the expression of HO-1 and significantly decreased the expression of VCAM-1 and COX-2 compared to the TNF-α control in mRNA level (P<0.05). These reasons of results might be due to the increased concentrations of procyanidin B1 (about 6-fold) and C1 (about 30-fold) produced through fermentation with Aspergillus oryzae NK for 48 h, at 37 °C. Overall, the results demonstrated that fSBE enhanced the inhibition of the inflammatory response and adherent molecule expression in HASMCs.

scholarly journals 4-(Phenylsulfanyl) Butan-2-One Attenuates the Inflammatory Response Induced by Amyloid-β Oligomers in Retinal Pigment Epithelium Cells

Marine Drugs ◽  
2020 ◽  
Vol 19 (1) ◽  
pp. 1
Author(s):  
Peeraporn Varinthra ◽  
Shun-Ping Huang ◽  
Supin Chompoopong ◽  
Zhi-Hong Wen ◽  
Ingrid Y. Liu
Keyword(s):  
Retinal Pigment Epithelium ◽  
Inflammatory Response ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Amyloid Β ◽  
Age Related Macular Degeneration ◽  
Epithelial Cell Line ◽  
Age Related ◽  
Tnf Α ◽  
Cox 2

Age-related macular degeneration (AMD) is a progressive eye disease that causes irreversible impairment of central vision, and effective treatment is not yet available. Extracellular accumulation of amyloid-beta (Aβ) in drusen that lie under the retinal pigment epithelium (RPE) has been reported as one of the early signs of AMD and was found in more than 60% of Alzheimer’s disease (AD) patients. Extracellular deposition of Aβ can induce the expression of inflammatory cytokines such as IL-1β, TNF-α, COX-2, and iNOS in RPE cells. Thus, finding a compound that can effectively reduce the inflammatory response may help the treatment of AMD. In this research, we investigated the anti-inflammatory effect of the coral-derived compound 4-(phenylsulfanyl) butan-2-one (4-PSB-2) on Aβ1-42 oligomer (oAβ1-42) added to the human adult retinal pigment epithelial cell line (ARPE-19). Our results demonstrated that 4-PSB-2 can decrease the elevated expressions of TNF-α, COX-2, and iNOS via NF-κB signaling in ARPE-19 cells treated with oAβ1-42 without causing any cytotoxicity or notable side effects. This study suggests that 4-PSB-2 is a promising drug candidate for attenuation of AMD.

Inflammatory response and matrix metalloproteinases in chronic kidney failure: Modulation by adropin and spexin

2021 ◽  
pp. 153537022110124
Author(s):  
Burak Yazgan ◽  
Filiz Avcı ◽  
Gülsün Memi ◽  
Ebru Tastekin
Keyword(s):  
Inflammatory Response ◽  
Kidney Failure ◽  
Renal Damage ◽  
Urine Volume ◽  
Kidney Tissue ◽  
Public Health Problem ◽  
Chronic Kidney Failure ◽  
Urine Protein ◽  
Cox 2 ◽  
Cox 1

Chronic kidney disease is a major global public health problem. The peptide hormones adropin and spexin modulate many physiological functions such as energy balance and glucose, lipid and protein metabolism. However, it is unclear whether these peptides may exert effects on renal damage, tissue remodeling, and inflammatory conditions. In view of the limited information, we aimed to investigate the effect of adropin and spexin on matrix metalloproteinase and inflammatory response genes a rat model of adenine-induced chronic kidney failure. Chronic kidney failure was induced in rats by administering adenine hemisulfate. Renal function was determined in an autoanalyzer. Histopathological modifications were assessed by H&E staining. mRNA expression levels of ALOX 15, COX 1, COX 2, IL-1β, IL-10, IL-17A, IL-18 IL-21, IL-33, KIM-1, MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, NGAL, TGFβ1, TIMP-1, and TNFα in kidney tissue were measured by qPCR. Our results showed an increase of 24-h urine volume, serum creatinine, BUN, and urine protein levels in group with adenine-induced CKF. Adropin and spexin treatments decreased urine protein and 24-h urine volume. Renal damage, TIMP-1, IL-33, and MMP-2 increased after CKF induction, while COX 1, MMP-9, and MMP-13 levels were significantly reduced. Furthermore, KIM-1, TIMP-1, IL-33, and MMP-2 were downregulated by spexin treatment. Renal damage, NGAL, TIMP-1 IL-17A, IL-33, MMP-2, and MMP-3 decreased after adropin treatment, while MMP-13 levels were upregulated. Treatment with adropin+spexin decreased KIM-1, NGAL, TIMP-1, IL-1β, IL-17A, IL-18, IL-33, ALOX 15, COX 1, COX 2, TGFβ1, TNFα, MMP-2, MMP-3, and MMP-7, but increased MMP-13 levels. Our findings revealed that inflammatory response and MMP genes were modulated by adropin and spexin. These peptides may have protective effects on inflammation and chronic kidney damage progression.

ChemInform Abstract: A Practical Synthesis of a COX-2-Specific Inhibitor.

ChemInform ◽  
2001 ◽  
Vol 32 (15) ◽  
pp. no-no
Author(s):  
Ian W. Davies ◽  
et al. et al.
Keyword(s):  
Specific Inhibitor ◽  
Practical Synthesis ◽  
Cox 2

scholarly journals Inorganic polyphosphates enhances nucleus pulposus tissue formation in vitro

2016 ◽  
Vol 35 (1) ◽  
pp. 41-50 ◽  
Author(s):  
Rahul Gawri ◽  
Toshikazu Shiba ◽  
Robert Pilliar ◽  
Rita Kandel
Keyword(s):  
Nucleus Pulposus ◽  
Tissue Formation ◽  
Inorganic Polyphosphates ◽  
Nucleus Pulposus Tissue

scholarly journals Creation of an injectable in situ gelling native extracellular matrix for nucleus pulposus tissue engineering

2017 ◽  
Vol 17 (3) ◽  
pp. 435-444 ◽  
Author(s):  
Rebecca A. Wachs ◽  
Ella N. Hoogenboezem ◽  
Hammad I. Huda ◽  
Shangjing Xin ◽  
Stacy L. Porvasnik ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Nucleus Pulposus ◽  
Nucleus Pulposus Tissue ◽  
In Situ Gelling

COX-2 and prostanoid expression in micturition pathways after cyclophosphamide-induced cystitis in the rat

2003 ◽  
Vol 284 (2) ◽  
pp. R574-R585 ◽  
Author(s):  
V. Y. Hu ◽  
S. Malley ◽  
A. Dattilio ◽  
J. B. Folsom ◽  
P. Zvara ◽  
...  
Keyword(s):  
Lower Urinary Tract ◽  
Specific Inhibitor ◽  
Female Rats ◽  
Prostaglandin E ◽  
Lower Urinary Tract Function ◽  
Tract Function ◽  
Cox 2 ◽  
Micturition Reflexes ◽  
Lower Urinary

The purpose of this study was to determine the role of cyclooxygenase-2 (COX-2) and its metabolites in lower urinary tract function after induction of acute (4 h), intermediate (48 h), or chronic (10 day) cyclophosphamide (CYP)-induced cystitis. Bladders were harvested from euthanized female rats for analyses. Conscious cystometry was used to assess the effects of a COX-2-specific inhibitor, 5,5-dimethyl-3-(3-fluorophenyl)-4-(4-methylsulfonyl)phenyl2(5 H)-furanone (DFU, 5 mg/kg sc), a disubstituted furanone, in CYP-induced cystitis. COX-2 mRNA was increased in inflamed bladders after acute (12-fold) and chronic (9-fold) treatment. COX-2 protein expression in inflamed bladders paralleled COX-2 mRNA expression. Prostaglandin D2-methoxime expression in the bladder was significantly ( P ≤ 0.01) increased in acute (3-fold) and chronic (5.5-fold) cystitis. Prostaglandin E2 was significantly ( P ≤ 0.01) increased (2-fold) in the bladder with intermediate (1.7-fold) and chronic (2.6-fold) cystitis. COX-2-immunoreactive cell profiles were distributed throughout the inflamed bladder and coexpressed histamine immunoreactivity. Conscious cystometry in rats treated with CYP + DFU showed increased micturition intervals 4 and 48 h after CYP treatment and decreased intravesical pressures during filling and micturition compared with rats treated with CYP + vehicle. These studies suggest an involvement of urinary bladder COX-2 and its metabolites in altered micturition reflexes with CYP-induced cystitis.

scholarly journals Eupatorium chinensis var. simplicifolium Root Extract Inhibits the Lipopolysaccharide-Induced Inflammatory Response in Raw 264.7 Macrophages by Inhibiting iNOS and COX-2 Expression

2012 ◽  
Vol 22 (9) ◽  
pp. 1137-1144
Author(s):  
Jin-Ho Lee ◽  
Dae-Hyun Kim ◽  
Ji-Won Shin ◽  
Sae-Jin Park ◽  
Yoon-Suk Kim ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Root Extract ◽  
Raw 264.7 ◽  
Raw 264.7 Macrophages ◽  
Cox 2

Effect of circumferential constraint on nucleus pulposus tissue in vitro

2010 ◽  
Vol 10 (2) ◽  
pp. 174-183 ◽  
Author(s):  
Darla J. Hamilton ◽  
Robert M. Pilliar ◽  
Stephen Waldman ◽  
Rita A. Kandel
Keyword(s):  
Nucleus Pulposus ◽  
Nucleus Pulposus Tissue
Sign in / Sign up

Export Citation Format

Share Document