Potassium efficiency of safflower (Carthamus tinctorius L.) and sunflower (Helianthus annuus L.)

2008 ◽  
Vol 171 (3) ◽  
pp. 431-439 ◽  
Author(s):  
Jóska Gerendás ◽  
Jehad Abbadi ◽  
Burkhard Sattelmacher
1998 ◽  
Vol 334 (2) ◽  
pp. 469-477 ◽  
Author(s):  
Dominic J. LACEY ◽  
Nikolaus WELLNER ◽  
Frederic BEAUDOIN ◽  
Johnathan A. NAPIER ◽  
Peter R. SHEWRY

Oil bodies were isolated from mature seeds of sunflower (Helianthus annuus L.) and safflower (Carthamus tinctorius L.). Oil body preparations containing only oleosin proteins could be obtained from safflower seeds by salt-washing followed by centrifugation on discontinuous sucrose density gradients. However, it was necessary to treat sunflower oil bodies with urea to obtain preparations of similar purity. Incubation of the oil bodies with proteinases gave two fragments with molecular masses of 6 and 8 kDa which were protected from digestion. These fragments represented the hydrophobic domain of the oleosins, as determined by N-terminal sequencing. Intact and proteinase-treated oil bodies of both species were analysed by Fourier-transform infrared spectroscopy, as dry films and in aqueous medium, the spectra being compared with those obtained for pure oil samples in order to identify the bands resulting from the oleosin proteins and protected peptides. This investigation showed that the hydrophobic domain of the oleosins in intact oil bodies is predominantly α-helical in structure and that the conformation was not greatly affected by washing the oil bodies with urea during preparation.


2018 ◽  
Vol 53 (3) ◽  
pp. 485-498
Author(s):  
G.P. Kononenko ◽  
◽  
M.I. Ustyuzhanina ◽  
A.A. Burkin ◽  
◽  
...  

2014 ◽  
Vol 2014 (2) ◽  
pp. 83-91
Author(s):  
Alfredo Garcia-Perez ◽  
◽  
Mark Harrison ◽  
Bill Grant ◽  
◽  
...  

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