Hyperimmunised bovine milk and whey: influence of pH and enzymatic treatments on the antigen-binding capacity of immunoglobulin G

Increased commercial demand for monoclonal antibodies (mAbs) has resulted in the urgent need to establish efficient production systems. We previously developed a transgenic chicken bioreactor system that effectively produced human cytokines in egg whites using genome-edited transgenic chickens. Here, we describe the application of this system to mAb production. The genes encoding the heavy and light chains of humanized anti-HER2 mAb, linked by a 2A peptide sequence, were integrated into the chicken ovalbumin gene locus using a CRISPR/Cas9 protocol. The knock-in hens produced a fully assembled humanized mAb in their eggs. The mAb expression level in the egg white was 1.4–1.9 mg/mL, as determined by ELISA. Furthermore, the antigen binding affinity of the anti-HER2 mAb obtained was estimated to be equal to that of the therapeutic anti-HER2 mAb (trastuzumab). In addition, antigen-specific binding by the egg white mAb was demonstrated by immunofluorescence against HER2-positive and -negative cells. These results indicate that the chicken bioreactor system can efficiently produce mAbs with antigen binding capacity and can serve as an alternative production system for commercial mAbs.

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Development of viper-venom antibodies in chicken egg yolk and assay of their antigen binding capacity

Toxicon ◽

10.1016/s0041-0101(01)00258-6 ◽

2002 ◽

Vol 40 (7) ◽

pp. 857-861 ◽

Cited By ~ 19

Author(s):

C Maya Devi ◽

M Vasantha Bai ◽

L.K Krishnan

Keyword(s):

Binding Capacity ◽

Egg Yolk ◽

Antigen Binding ◽

Chicken Egg ◽

Chicken Egg Yolk

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The Hepatitis B Surface Antigen Binding Protein: An Immunoglobulin G Constant Region-Like Protein That Interacts With HBV Envelop Proteins and Mediates HBV Entry

Frontiers in Cellular and Infection Microbiology ◽

10.3389/fcimb.2018.00338 ◽

2018 ◽

Vol 8 ◽

Cited By ~ 3

Author(s):

Yeping Sun ◽

Shanshan Wang ◽

Yong Yi ◽

Jing Zhang ◽

Zhongping Duan ◽

...

Keyword(s):

Hepatitis B ◽

Surface Antigen ◽

Immunoglobulin G ◽

Binding Protein ◽

Hepatitis B Surface Antigen ◽

Antigen Binding ◽

Constant Region

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Cyclic peptide ligand with high binding capacity for affinity purification of immunoglobulin G

Journal of Chromatography A ◽

10.1016/j.chroma.2016.09.007 ◽

2016 ◽

Vol 1466 ◽

pp. 105-112 ◽

Cited By ~ 12

Author(s):

Hyo Jin Kang ◽

Weonu Choe ◽

Jeong-Ki Min ◽

Young-mi Lee ◽

B. Moon Kim ◽

...

Keyword(s):

Immunoglobulin G ◽

Cyclic Peptide ◽

Binding Capacity ◽

Affinity Purification ◽

Peptide Ligand ◽

High Binding

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Influence of pH on the chloride binding capacity of Limestone Calcined Clay Cements (LC3)

Cement and Concrete Research ◽

10.1016/j.cemconres.2020.106031 ◽

2020 ◽

Vol 131 ◽

pp. 106031 ◽

Cited By ~ 4

Author(s):

François Avet ◽

Karen Scrivener

Keyword(s):

Binding Capacity ◽

Chloride Binding ◽

Calcined Clay ◽

Influence Of Ph

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A Radioimmunoassay of the Antigen-Binding Capacity of Rabbit Antiserum Prepared with Axenically Grown Entamoeba histolytica, Laredo Strain

Journal of Parasitology ◽

10.2307/3278437 ◽

1973 ◽

Vol 59 (5) ◽

pp. 927 ◽

Cited By ~ 1

Author(s):

Bernard Halpern ◽

Richard A. Albach ◽

James G. Shaffer ◽

Ralph E. Dolkart

Keyword(s):

Entamoeba Histolytica ◽

Binding Capacity ◽

Rabbit Antiserum ◽

Antigen Binding

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Recombinant Escherichia coli Strain Produces a ZZ Domain Displaying Biopolyester Granules Suitable for Immunoglobulin G Purification

Applied and Environmental Microbiology ◽

10.1128/aem.01014-06 ◽

2006 ◽

Vol 72 (11) ◽

pp. 7394-7397 ◽

Cited By ~ 47

Author(s):

Jane A. Brockelbank ◽

Verena Peters ◽

Bernd H. A. Rehm

Keyword(s):

Escherichia Coli ◽

Immunoglobulin G ◽

Binding Capacity ◽

Protein A ◽

Coli Strain ◽

Pha Synthase ◽

Enzyme Linked Immunosorbent Assay ◽

Ionization Time ◽

Flight Mass Spectrometry ◽

Igg Binding

ABSTRACT The immunoglobulin G (IgG) binding ZZ domain of protein A from Staphylococcus aureus was fused to the N terminus of the polyhydroxyalkanoate (PHA) synthase from Cupriavidus necator. The fusion protein was confirmed by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry and mediated formation of ZZ domain-displaying PHA granules in recombinant Escherichia coli. The IgG binding capacity of isolated granules was assessed using enzyme-linked immunosorbent assay and could be enhanced by the overproduction of the ZZ-PHA synthase. ZZ-PHA granules enabled efficient purification of IgG from human serum.

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