Isolation of immunoglobulin G from bovine milk whey by poly(hydroxyethyl methacrylate)-based anion-exchange cryogel

2013 ◽  
Vol 36 (15) ◽  
pp. 2387-2393 ◽  
Author(s):  
Shasha Dong ◽  
Liang Chen ◽  
Bin Dai ◽  
Wilfred Johnson ◽  
Jialei Ye ◽  
...  
Keyword(s):  
Anion Exchange ◽  
Immunoglobulin G ◽  
Bovine Milk ◽  
Hydroxyethyl Methacrylate ◽  
Milk Whey

Chromatographic purification of immunoglobulin G from bovine milk whey

1994 ◽  
Vol 673 (1) ◽  
pp. 45-53 ◽  
Author(s):  
Premysl Konecny ◽  
Rodney J. Brown ◽  
William H. Scouten
Keyword(s):  
Immunoglobulin G ◽  
Bovine Milk ◽  
Chromatographic Purification ◽  
Milk Whey

scholarly journals Immunoglobulin G from bovine milk primes intestinal epithelial cells for increased colonization of bifidobacteria

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Sinead T. Morrin ◽  
Geoffrey McCarthy ◽  
Deirdre Kennedy ◽  
Mariarosaria Marotta ◽  
Jane A. Irwin ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Immunoglobulin G ◽  
Intestinal Epithelial Cells ◽  
Bovine Milk ◽  
Intestinal Epithelial

Immunomodulatory activities of whey fractions in efferent prefemoral lymph of sheep

1996 ◽  
Vol 63 (2) ◽  
pp. 257-267 ◽  
Author(s):  
Chun W. Wong ◽  
Geoffrey O. Regester ◽  
Geoffrey L. Francis ◽  
Dennis L. Watson
Keyword(s):  
Immune Responses ◽  
Bovine Milk ◽  
Inhibitory Effect ◽  
Dependent Manner ◽  
Milk Whey ◽  
Significant Difference ◽  
Lymphocyte Phenotypes ◽  
Dose Dependent

SummaryStudies on the immunomodulatory activities of ruminant milk and colostral whey fractions were undertaken. By comparing with boiled colostral whey in a preliminary experiment, a putative heat-labile immunostimulatory factor for antibody responses was found to be present in ovine colostral whey. Studies were then undertaken in sheep in which the efferent prefemoral lymphatic ducts were cannulated bilaterally, and immune responses in the node were measured following subcutaneous injection in the flank fold of whey protein preparations of various purities. A significant sustained decline of efferent lymphocyte output was observed following injection with autologous crude milk whey or colostral whey preparations, but no changes were observed in interferon-gamma levels in lymph plasma. Two bovine milk whey fractions (lactoperoxidase and lactoferrin) of high purity were compared in bilaterally cannulated sheep. A transient decline over the first 6 h was seen in the efferent lymphocyte output and lymph flow rate after injection of both fractions. A significant difference was seen between the two fractions in interferongamma levels in lymph at 6 h after injection. However, no significant changes in the proportion of the various efferent lymphocyte phenotypes were seen following either treatment. Whereas both fractions showed a significant inhibitory effect in a dose-dependent manner on the proliferative response of T lymphocytes, but not B lymphocytes, to mitogenic stimulation in vitro, no similar changes were seen following in vivo stimulation with these two fractions.

scholarly journals The effect of whey acidic protein fractions on bone loss in the ovariectomised rat

2005 ◽  
Vol 94 (2) ◽  
pp. 244-252 ◽  
Author(s):  
Marlena C. Kruger ◽  
Gabrielle G. Plimmer ◽  
Linda M. Schollum ◽  
Neill Haggarty ◽  
Satyendra Ram ◽  
...  
Keyword(s):  
Bone Mineral Density ◽  
Bone Loss ◽  
Bovine Milk ◽  
Whey Acidic Protein ◽  
Acidic Protein ◽  
Protein Fractions ◽  
Female Rats ◽  
Mineral Density ◽  
Milk Whey ◽  
Over Time

Bovine milk has been shown to contain bioactive components with bone-protective properties. Earlier studies on bovine milk whey protein showed that it suppressed bone resorption in the female ovariectomised rat. A new osteotropic component was subsequently identified in the whey basic protein fraction, but bone bioactivity may also be associated with other whey fractions. In the present study, we investigated whether acidic protein fractions isolated from bovine milk whey could prevent bone loss in mature ovariectomised female rats. Six-month-old female rats were ovariectomised (OVX) or left intact (sham). The OVX rats were randomised into four groups. One group remained the control (OVX), whereas three groups were fed various whey acidic protein fractions from milk whey as 3g/kg diet for 4 months. Outcomes were bone mineral density, bone biomechanics and markers of bone turnover. Bone mineral density of the femurs indicated that one of the whey AF over time caused a recovery of bone lost from OVX. Plasma C-telopeptide of type I collagen decreased significantly in all groups except OVX control over time, indicating an anti-resorptive effect of whey acidic protein. Biomechanical data showed that the AF may affect bone architecture as elasticity was increased by one of the whey AF. The femurs of AF-supplemented rats all showed an increase in organic matter. This is the first report of an acidic whey protein fraction isolated from milk whey that may support the recovery of bone loss in vivo.

Transferrin glycosylation in hypoxia

1991 ◽  
Vol 69 (4) ◽  
pp. 239-244 ◽  
Author(s):  
Erwin Regoeczi ◽  
J. Michael Kay ◽  
Paul A. Chindemi ◽  
Ouahida Zaimi ◽  
Kaye L. Suyama
Keyword(s):  
Affinity Chromatography ◽  
Sea Level ◽  
Anion Exchange ◽  
Immunoglobulin G ◽  
Lectin Affinity Chromatography ◽  
Lectin Affinity ◽  
Hypobaric Chamber

The aim of this study was to examine the effect of reduced O2 tension on the glycosylation of transferrin. Rats were placed in a hypobaric chamber (380 mmHg) that corresponded to an altitude of 5486 m above sea level for 21 days. The animals responded with marked increases in hematocrit (from 44 to 76%) and cardiac weight, and with reductions in the concentration of plasma transferrin averaging 15%. Analyses of their plasma transferrin by serial anion-exchange and lectin affinity chromatography revealed no changes in the extent of glycan branching. However, there was a moderate rise in the proportion of fucosylated transferrin molecules (fucosylation index) and a slight decrease in the transferrin fraction bearing a tetrasialylated biantennary glycan. The fucosylation index correlated positively with plasma transferrin concentrations in the test animals, but not in the controls. In contradistinction to the situation with transferrin, hypoxic rats exhibited a reduced fucosylation index of immunoglobulin G.Key words: fucosylation index, hypoxia, immunoglobulin G, lectin affinity chromatography, transferrin.

Study of the interactions between rosmarinic acid and bovine milk whey protein α-Lactalbumin, β-Lactoglobulin and Lactoferrin

2015 ◽  
Vol 77 ◽  
pp. 450-459 ◽  
Author(s):  
Vincenza Ferraro ◽  
Ana Raquel Madureira ◽  
Bruno Sarmento ◽  
Ana Gomes ◽  
Manuela E. Pintado
Keyword(s):  
Whey Protein ◽  
Rosmarinic Acid ◽  
Bovine Milk ◽  
Milk Whey ◽  
Β Lactoglobulin ◽  
Milk Whey Protein

Immune modulation in suckling rat pups by a growth factor extract derived from milk whey

2001 ◽  
Vol 68 (4) ◽  
pp. 587-599 ◽  
Author(s):  
IRMELI A. PENTTILA ◽  
MIN F. ZHANG ◽  
EDNA BATES ◽  
GEOFFREY REGESTER ◽  
LEANNA C. READ ◽  
...  
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Immune Modulation ◽  
Oral Tolerance ◽  
Specific Antigen ◽  
Bovine Milk ◽  
Maternal Milk ◽  
Milk Whey ◽  
Rat Pups ◽  
Food Antigens

Oral tolerance to foreign enteral antigens is not fully developed in early neonatal life. Epidemiological evidence supports a role for maternal milk in the development of immune responses, including oral tolerance. Formula fed infants have an increased susceptibility to food allergy and the later development of autoimmune disease. This may relate to the lack in infant formula of growth factors found in maternal milk. Bovine milk contains proteins, growth factors and cytokines. Various studies have outlined the immune modulating potential of bovine milk-derived products. Fractionated whey extracts have therapeutic potential in disease states where there is an excessive inflammatory reaction, and disease preventive potential for infants who are not breast-fed. We have shown that daily oral administration of a growth factor-enriched fraction from milk whey to naturally suckling rat pups between days 4–9 postnatal can down-regulate immune activation to a specific orally administered food antigen, ovalbumin, assessed by lymphocyte proliferation. In addition, non-specific down-regulation in the intestine was observed as assessed by the expression of MHC I. Treatment of rat pups with whey extract at the time of oral sensitisation to ovalbumin also resulted in an increased secretion of TGF-β into the culture supernatant of spleen cells incubated with specific antigen. TGF-β is an immuno-down-regulatory cytokine involved in tolerance induction. Immune modulation by extracts derived from milk whey could be of potential benefit for formula-fed and pre-term infants in reducing susceptibility to inappropriate activation to food antigens.

Bovine lactoferrin and its functions in animals -A review

2015 ◽  
Vol 36 (4) ◽  
Author(s):  
Rajan Sharma ◽  
Dibyendu Chakraborty ◽  
Parul Gupta
Keyword(s):  
Bos Taurus ◽  
Bovine Milk ◽  
Regulatory Elements ◽  
Bovine Lactoferrin ◽  
Milk Whey ◽  
Lactoferrin Gene ◽  
Exon 1 ◽  
Variable Content ◽  
Differential Gene ◽  
Strong Antimicrobial Activity

Lactoferrin (Lf) was discovered in 1939 as “red protein from milk whey”. Bovine lactoferrin (bLf) gene is located on <italic>Bos taurus</italic> autosome, long arm of chromosome no.22 (BTA 22q24) in cattle. Its size varies from 23-35kbp among different species. The lactoferrin gene consists of 17 exons and 16 introns ranging from 82bp (exon-1) to 225bp (exon-17). The presence of multiple regulatory elements within lactoferrin promoter contributes differential gene expression and variable content of lactoferrin in milk. The concentration of lactoferrin in normal bovine milk is about 0.02-0.2 mg/ml. The primary function of Lf lies in its role in iron metabolism including iron transport, storage and chelation. Lf exhibits strong antimicrobial activity against a broad spectrum of bacteria (gram-positive & negative), fungi, yeasts, viruses and parasites. Lf exerts bacteriostatic and bactericidal activity. Its main contribution to antiviral defence consists in its binding to the cell membrane glycosaminoglycan, thus lactoferrin prevents viruses from entering cells and infection is stopped at an early age. More than 140 SNPs in this gene have been identiûed. Such a high variability in Lactoferrin gene implies that it may be used as candidate gene for screening animals also a marker of milk yield.

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