Background:
Phospholipase D (PLD)is closely related to browning and senescence of
postharvest longan fruit.
Objective:
This study investigated the effects of 2-butanol (a PLD inhibitor) on the expression and
regulation of PLD during storage of longan fruit at a low temperature.
Methods:
Senescence-related quality indices showed that the 2-butanol-treated fruit presented lower
pericarp browning index, pulp breakdown index and total soluble solid value than the untreated
fruit.
Results:
The fruit treated by 60 µL/L 2-butanol exhibited the strongest inhibition on senescence,
which significantly delayed changes in weight, titratable acidity content, total soluble solid content
and ascorbic acid content. This treatment maintained a high level of total phenolic content and
caused significant inhibition on pericarp browning and pulp breakdown. Through ELISA method,
60 µL/L 2-butanol treatment also reduced PLD activity. Real-time RT-PCR (RT-qPCR) results
showed that PLD mRNA expression level was inhibited by 60 µL/L 2-butanol within 15 days.
Western-blotting results further confirmed the differential expression of PLD during storage, and a
relatively higher expression for PLD protein was found in control compared to the 2-butanoltreated
fruit during 15-d storage.
Conclusion:
These results provided a scientific basis and reference to further investigating postharvest
longan quality maintenance by regulating the PLD gene expression.
Application of hyperspectral imaging for spatial prediction of soluble solid content in sweet potato
