Role of Phospholipase D Inhibitor in Regulating Expression of Senescencerelated Phospholipase D gene in Postharvest Longan Fruit

2019 ◽  
Vol 14 (7) ◽  
pp. 649-657 ◽  
Author(s):  
Li Li ◽  
Jiemin Li ◽  
Jian Sun ◽  
Ping Yi ◽  
Changbao Li ◽  
...  
Keyword(s):  
Phospholipase D ◽  
Ascorbic Acid Content ◽  
Titratable Acidity ◽  
Significant Inhibition ◽  
Solid Content ◽  
Total Soluble Solid ◽  
Total Phenolic ◽  
Soluble Solid Content ◽  
Soluble Solid ◽  
Pericarp Browning

Background: Phospholipase D (PLD)is closely related to browning and senescence of postharvest longan fruit. Objective: This study investigated the effects of 2-butanol (a PLD inhibitor) on the expression and regulation of PLD during storage of longan fruit at a low temperature. Methods: Senescence-related quality indices showed that the 2-butanol-treated fruit presented lower pericarp browning index, pulp breakdown index and total soluble solid value than the untreated fruit. Results: The fruit treated by 60 µL/L 2-butanol exhibited the strongest inhibition on senescence, which significantly delayed changes in weight, titratable acidity content, total soluble solid content and ascorbic acid content. This treatment maintained a high level of total phenolic content and caused significant inhibition on pericarp browning and pulp breakdown. Through ELISA method, 60 µL/L 2-butanol treatment also reduced PLD activity. Real-time RT-PCR (RT-qPCR) results showed that PLD mRNA expression level was inhibited by 60 µL/L 2-butanol within 15 days. Western-blotting results further confirmed the differential expression of PLD during storage, and a relatively higher expression for PLD protein was found in control compared to the 2-butanoltreated fruit during 15-d storage. Conclusion: These results provided a scientific basis and reference to further investigating postharvest longan quality maintenance by regulating the PLD gene expression.

scholarly journals Endogenous pH, Titratable Acidity and Total Soluble Solid Content of Mouthwashes Available in the Brazilian Market

2010 ◽  
Vol 04 (02) ◽  
pp. 156-159 ◽  
Author(s):  
Alessandro Leite Cavalcanti ◽  
Ianny Alves Ramos ◽  
Rafaela Bastos Leite ◽  
Mariana da Costa Oliveira ◽  
Karynna de Melo Menezes ◽  
...  
Keyword(s):  
Titratable Acidity ◽  
Critical Value ◽  
Solid Content ◽  
Total Soluble Solid ◽  
Soluble Solid Content ◽  
Dental Tissues ◽  
Ph Values ◽  
Soluble Solid ◽  
Abbe Refractometer

Objectives: To evaluate in vitro the endogenous pH, titratable acidity and total soluble solid content (TSSC) of mouthwashes available in the Brazilian market.Methods: The study sample was composed of 10 commercial brands of mouthwashes based on different active ingredients: Cepacol®, Clinerize®, Equate®, Listerine Cool Citrus®, Oral-B®, Periogard ®, Peroxyl®, Plax Overnight®, Prevident 220® and Sanifill®. The experiments were performed in triplicate. The endogenous pH was evaluated by potentiometry, titratable acidity was evaluated by the addition of 0.1N KOH increments to the mouthwashes, and TSSC readings were performed by Brix refractometry using the Abbé refractometer.Results: pH values ranged from 3.56 (Peroxyl®) to 7.43 (Cepacol®) and three mouthwashes presented pHs below 5.5. The titratable acidity values ranged from 0.007 (Periograd®) to 0.530 (Prevident ®). Oral B® and Clinerize® presented the lowest (4.7%) and the highest (23.70%) TSSC, respectively.Conclusions: Some of the mouthwashes evaluated in this study presented low endogenous pH, even below the critical value for enamel dissolution (pH<5.5), high titratable acidity and high TSSC, and may be potentially erosive to the dental tissues if not properly used. (Eur J Dent 2010;4:156-159)

scholarly journals Phytochemical changes in heated Rosa species fruits and seeds

2017 ◽  
Vol 35 (No. 4) ◽  
pp. 345-351
Author(s):  
Güneş Mehmet ◽  
Dölek Ümit ◽  
Elmastaş Mahfuz
Keyword(s):  
Vitamin C ◽  
Antioxidant Activities ◽  
Titratable Acidity ◽  
Solid Content ◽  
Total Sugar ◽  
Total Phenolic ◽  
Soluble Solid Content ◽  
Soluble Solid ◽  
Rosa Species ◽  
Β Carotene

The phytochemical changes in heat-treated Rosa species fruits and seeds were determined. Fruits and seeds of five advanced genotypes belonging to Rosa dumalis, R. canina, and R. villosa were used. Fruits were harvested at optimal maturity and boiled in distilled water for 30 min, followed by analysis of soluble solid content, dry matter, pH, titratable acidity, total sugar, β-carotene, α-tocopherol, and vitamin C. Total phenolic content and the antioxidant activities of fresh and boiled fruits were also analyzed, and fatty acid levels in fresh and heated seeds were determined. Total sugar, pH, and antioxidant activities were not affected, but β-carotene was affected significantly by heating in all species. The losses in β-carotene, α-tocopherol, and vitamin C were between 78–86, 29–51, and 12–60%, respectively. Heat treatment did not affect significantly the ratios of major fatty acids except for R. dumalis (MR-12 and MR-46).

scholarly journals Nitrogen fertilization associated with cold storage and its impacts on the maintenance of peach quality

2020 ◽  
Vol 36 (3) ◽  
Author(s):  
Caroline Farias Barreto ◽  
Renan Navroski ◽  
Letícia Vanni Ferreira ◽  
Jorge Atílio Benati ◽  
Marcelo Barbosa Malgarim ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Cold Storage ◽  
Titratable Acidity ◽  
N Fertilization ◽  
Solid Content ◽  
Total Phenolic ◽  
Soluble Solid Content ◽  
Soluble Solid ◽  
Peach Trees

Nitrogen, which is considered the most important nutrient for peach trees, may interfere in both quantitative production characteristics and quality of fruits. This study aimed at evaluating the effect of the combination of doses of N fertilization and different periods of cold storage on physico-chemical and phytochemical characteristics of peaches in post-harvest. The experiment had a randomized complete block design in a 4x3 factorial scheme, i. e., four doses of fertilization (0, 60, 120 and 180 Kg N ha-1) and three periods of storage (on the harvest day, on both the 15th and the 30th storage days at 1±1ºC, each followed by a day of simulated commercialization at 20±1ºC). The following aspects were evaluated in fruits yielded by peach trees of the genotype Cascata 1067: fruit color, soluble solid content, titratable acidity, pulp firmness, mass loss, total phenolic compounds and antioxidant activity. Different periods of cold storage and doses of N fertilization were found to affect epidermis luminosity, pulp firmness and titratable acidity of fruits. Peaches may be stored at low temperatures for 15+1 days. After that, loss of fruit firmness increases. N fertilization affects neither the soluble solid content nor the epidermis color of peaches, but both parameters are influenced by storage. Values of phenolic compounds and antioxidant activity decrease when there is N increment in the soil and when longer storage is carried out. Results suggest that peach composition may be affected by cultural practices, such as N fertilization, in harvest and after storage.       

scholarly journals Comparative ANN-TLBO and RSM Optimisation Approach for Bioactive Potential of Microwave Convective Dried Mango (Mangifera indica)

Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 1
Author(s):  
Tanmay Sarkar ◽  
Molla Salauddin ◽  
Sudipta Kumar Hazra ◽  
Runu Chakraborty
Keyword(s):  
Mangifera Indica ◽  
Power Level ◽  
Back Propagation ◽  
Solid Content ◽  
Total Soluble Solid ◽  
Convective Drying ◽  
Total Phenolic ◽  
Soluble Solid Content ◽  
Process Conditions ◽  
Soluble Solid

The mango (Mangifera indica) is a rich source of antioxidants like phenolic acids and flavonoids. Polyphenol oxidase (PPO) and polyphenol peroxidase (POD) enzymes oxidize mono and diphenols, and thus the antioxidant potential diminishes. For cost-effective and sustainable preservation of this sub-tropical climacteric fruit, microwave convective dehydration is a prospective choice. Drying operations deactivate PPO and POD. Microwave convective drying was done at 100–300 W of power in a temperature range of 40–80 °C, with a total soluble solid content of 20–30 °B and a puree load of 0.4–0.6 g/cm2 to investigate the effect of the process parameters on the total phenolic content (TPC) of the final product. Response surface methodology (RSM) was employed to optimize the TPC value. Furthermore, an artificial neural network (ANN) with the back propagation-feed forward modeling approach was adopted for the experimental results obtained, teaching–learning-based optimization (TLBO) was then employed to acquire the optimized drying condition for samples with the maximum TPC value. The optimized process conditions obtained from both methods were virtually compatible. From RSM analysis, the maximum TPC value of 13.36 mg GAE/100 g was observed at a 170.27 W power level, 57.84 °C oven temperature, 0.60 g/cm2 of puree load and 29.05 °B total soluble solid content, whereas from the ANN-TLBO technique, 14.29 mg GAE/100 g of TPC was attained for the following combination of drying parameters: 175.08 W, 57.15 °C, 0.60 g/cm2 and 29.25 °B. The ANN-TLBO approach predicted a more optimized result (TPC value) in comparison with the RSM method.

scholarly journals Effect of Cross-Linked Alginate/Oil Nanoemulsion Coating on Cracking and Quality Parameters of Sweet Cherries

Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 449
Author(s):  
Camilo Gutiérrez-Jara ◽  
Cristina Bilbao-Sainz ◽  
Tara McHugh ◽  
Bor-Sen Chiou ◽  
Tina Williams ◽  
...  
Keyword(s):  
Titratable Acidity ◽  
Quality Parameters ◽  
Solid Content ◽  
Postharvest Quality ◽  
Soluble Solid Content ◽  
Nutritional Values ◽  
Quality Properties ◽  
Vapor Transfer ◽  
Sweet Cherries ◽  
Soluble Solid

The cracking of sweet cherries causes significant crop losses. Sweet cherries (cv. Bing) were coated by electro-spraying with an edible nanoemulsion (NE) of alginate and soybean oil with or without a CaCl2 cross-linker to reduce cracking. Coated sweet cherries were stored at 4 °C for 28 d. The barrier and fruit quality properties and nutritional values of the coated cherries were evaluated and compared with those of uncoated sweet cherries. Sweet cherries coated with NE + CaCl2 increased cracking tolerance by 53% and increased firmness. However, coated sweet cherries exhibited a 10% increase in water loss after 28 d due to decreased resistance to water vapor transfer. Coated sweet cherries showed a higher soluble solid content, titratable acidity, antioxidant capacity, and total soluble phenolic content compared with uncoated sweet cherries. Therefore, the use of the NE + CaCl2 coating on sweet cherries can help reduce cracking and maintain their postharvest quality.

scholarly journals The Mater-Bi® biodegradable film for strawberry (Fragaria x ananassa Duch.) mulching: effects on fruit yield and quality

2016 ◽  
Vol 11 (3) ◽  
pp. 203 ◽  
Author(s):  
Luigi Morra ◽  
Maurizio Bilotto ◽  
Domenico Cerrato ◽  
Raffaella Coppola ◽  
Vincenzo Leone ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Titratable Acidity ◽  
Solid Content ◽  
Soluble Solid Content ◽  
Chemical Parameters ◽  
Biodegradable Film ◽  
Yield And Quality ◽  
Physical Chemical ◽  
Soluble Solid

Two trials in different agricultural farms were carried out from October 2014 to June 2015 with the aim to assess the advantages linked to the substitution of the low density polyethylene (LDPE) films for soil mulching with the Mater-Bi<sup>®</sup> biodegradable films in the strawberry cultivation under tunnel in Campania. Lifetime of biodegradable mulch and influence of type of mulch on the yield and the quality of cvs Sabrina and Fortuna were evaluated. Plants were cultivated on mulched, raised beds, high 40 cm from bottom soil. Mater-Bi<sup>®</sup> film was 20 <span>µ</span>m thick while LDPE film was 50 mm thick. The physical-chemical parameters (firmness, pH, total soluble solid content, titratable acidity and skin colour) and some bioactive compounds (total polyphenols, flavonoids, anthocyanins, antioxidant activity) of fruits were determined by three samplings effected in consecutive months (from March to May 2015) of the harvest cycle. Biodegradable film guaranteed an effective mulch along the whole strawberry cycle (9-10 months including the time of drawing up of film). Yields of cv Sabrina on LDPE was 18% higher than those on Mater-Bi<sup>®</sup> while the opposite was detected in cv Fortuna (+10%). The physical-chemical parameters of fruits were not modified by the mulches. The content of the bioactive compounds, instead, resulted, in each time of sampling, significantly higher in fruits picked on Mater-Bi<sup>®</sup> based film.

scholarly journals Evaluation of production conditions of tomato grafted with different tobacco rootstocks and determining nicotine content and quality of fruit

2020 ◽  
Vol 115 (2) ◽  
pp. 297
Author(s):  
Seda TUNÇAY ÇAĞATAY ◽  
Gülşah ÇALIK KOÇ ◽  
Fereshteh REZAEİ ◽  
Özlem DARCANSOY İŞERI ◽  
Feride İffet ŞAHIN ◽  
...  
Keyword(s):  
Fruit Size ◽  
Titratable Acidity ◽  
Solid Content ◽  
Soluble Solid Content ◽  
Quality Factors ◽  
Nicotine Content ◽  
Positive Effects ◽  
Tomato Yield ◽  
Significant Difference ◽  
Soluble Solid

<p>This study aimed to investigate the effects of grafting tomato on different tobacco rootstocks on quality factors and nicotine content. The commercial variety (Solanum lycopersicum ‘H2274’) (BIOTECH) of the tomato was used as the scion plant, and six different tobacco (Nicotiana tabacum L.) rootstocks were used: Taşova, Tekel, Muş, Samsun, Dişbudak, Hasankeyf cultivars. Cleft grafting method was used in all plants. Yield of non-grafted and grafted plants grown in open-field conditions was calculated, and there was a significant increase in yield in grafted tomatoes compared to non-grafted tomatoes. There was significantly increased lycopene and β-carotene levels (mg kg-1), especially in ‘Tekel’, ‘Taşova’, ‘Samsun’, and ‘Hasankeyf’ tobacco grafts. There was a statistically significant difference between grafted and non-grafted plants according to 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical retention capacities and total phenol (TP) values. Evaluation of quality determinants including pH values, titratable acidity values (citric acid %), soluble solid content (SSC)(oBrix) , fruit size ratios, showed that tomatoes grafted with ‘Muş’ tobacco rootstock were of higher quality. There was no significant difference between grafted and non-grafted plants according to nicotine analysis of the tobacco-grafted tomatoes, and due to acceptable ranges of nicotin level on tobacco grafted tomato plants were considered to be suitable for consumption. It could be concluded that grafting practices have significantly positive effects on tomato yield and quality.<br /><br /></p>

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