Development of a new method for improved identification and relative quantification of unknown metabolites in complex samples: Determination of a triterpenoid metabolic fingerprint for the in situ characterization of Ganoderma bioactive compounds

2009 ◽  
Vol 32 (23-24) ◽  
pp. 4052-4058 ◽  
Author(s):  
Jiri Adamec ◽  
Amber Jannasch ◽  
Shailesh Dudhgaonkar ◽  
Andrej Jedinak ◽  
Miroslav Sedlak ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
New Method ◽  
Relative Quantification ◽  
In Situ Characterization ◽  
Complex Samples ◽  
Metabolic Fingerprint

scholarly journals How Reliable Is the Electrochemical Readout of MIP Sensors?

Sensors ◽  
2020 ◽  
Vol 20 (9) ◽  
pp. 2677 ◽  
Author(s):  
Aysu Yarman ◽  
Frieder W. Scheller
Keyword(s):  
Specific Binding ◽  
In Situ Characterization ◽  
Faradaic Current ◽  
Diffusional Permeability ◽  
Redox Active ◽  
Nonspecific Interactions ◽  
Target Binding

Electrochemical methods offer the simple characterization of the synthesis of molecularly imprinted polymers (MIPs) and the readouts of target binding. The binding of electroinactive analytes can be detected indirectly by their modulating effect on the diffusional permeability of a redox marker through thin MIP films. However, this process generates an overall signal, which may include nonspecific interactions with the nonimprinted surface and adsorption at the electrode surface in addition to (specific) binding to the cavities. Redox-active low-molecular-weight targets and metalloproteins enable a more specific direct quantification of their binding to MIPs by measuring the faradaic current. The in situ characterization of enzymes, MIP-based mimics of redox enzymes or enzyme-labeled targets, is based on the indication of an electroactive product. This approach allows the determination of both the activity of the bio(mimetic) catalyst and of the substrate concentration.

scholarly journals NON-DESTRUCTIVE METHOD FOR RAPID "IN SITU" CHARACTERIZATION OF ROCKS

2004 ◽  
Vol 36 (4) ◽  
pp. 1912
Author(s):  
N. Ciornei ◽  
I. Facaoaru ◽  
V. Cetean
Keyword(s):  
Quality Criteria ◽  
In Situ Characterization ◽  
Hard Rocks ◽  
Determination Process ◽  
Building Activity ◽  
Non Destructive ◽  
Selection Of

The effective determination of the field and place of application of the products resulting from stone working is a compulsory requirement in the building activity and is solved by laboratory analyses. This article presents details on the determination, by non-destructive method, of the compression strength of the rocks, both "in situ" (in the deposit) and in a laboratory. The originality of the method consists in simplifying the determination process of this parameter, a method that is in agreement with the systems proposed to be applied in the European Community. The method has the advantage of being applicable in quarries, where knowledge of this parameter leads to a rationalizing of the working and more especially to a selection of the blocks by quality criteria. The method is applicable to all categories of natural consolidated hard rocks, being a viable alternative for deposits with an inhomogeneous composition as to quality and structure.

New method for in situ characterization of loose material for landslide mapping purpose

2007 ◽  
Vol 94 (3-4) ◽  
pp. 166-179 ◽  
Author(s):  
Adrien Pantet ◽  
Aurèle Parriaux ◽  
Philippe Thelin
Keyword(s):  
Loose Material ◽  
New Method ◽  
In Situ Characterization ◽  
Landslide Mapping ◽  
Mapping Purpose

Characterization of the glycoconjugates of boar testis and epididymis

Reproduction ◽  
2000 ◽  
pp. 325-335 ◽  
Author(s):  
A Calvo ◽  
LM Pastor ◽  
S Bonet ◽  
E Pinart ◽  
M Ventura
Keyword(s):  
Ricinus Communis ◽  
Lectin Histochemistry ◽  
Apical Part ◽  
Seminiferous Tubules ◽  
In Situ Characterization ◽  
Intense Staining ◽  
Terminal Galactose ◽  
Boar Spermatozoa

Lectin histochemistry was used to perform in situ characterization of the glycoconjugates present in boar testis and epididymis. Thirteen horseradish peroxidase- or digoxigenin-labelled lectins were used in samples obtained from healthy fertile boars. The acrosomes of the spermatids were stained intensely by lectins with affinity for galactose and N-acetyl-galactosamine residues, these being soybean, peanut and Ricinus communis agglutinins. Sertoli cells were stained selectively by Maackia ammurensis agglutinin. The lamina propria of seminiferous tubules showed the most intense staining with fucose-binding lectins. The Golgi area and the apical part of the principal cells of the epididymis were stained intensely with many lectins and their distribution was similar in the three zones of the epididymis. On the basis of lectin affinity, both testis and epididymis appear to have N- and O-linked glycoconjugates. Spermatozoa from different epididymal regions showed different expression of terminal galactose and N-acetyl-galactosamine. Sialic acid (specifically alpha2,3 neuraminic-5 acid) was probably incorporated into spermatozoa along the extratesticular ducts. These findings indicate that the development and maturation of boar spermatozoa are accompanied by changes in glycoconjugates. As some lectins stain cellular or extracellular compartments specifically, these lectins could be useful markers in histopathological evaluation of diseases of boar testis and epididymis.

In Situ Characterization of Soils for Prediction of Stress-Strain Relationship

1983 ◽  
Author(s):  
K. Arulanandan ◽  
Y. Dafalias ◽  
L. R. Herrmann ◽  
A. Anandarajah ◽  
N. Meegoda
Keyword(s):  
In Situ Characterization ◽  
Stress Strain ◽  
Strain Relationship
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