Polymer monoliths for the concentration of viruses from environmental waters. A review

A fluorogenic β-glucuronidase assay comprising membrane filtration followed by selective enumeration on m-FC agar at 44.5°C and further confirmation using tlie 4-metliylumbelliferyl-β-D-glucuronide (MUG) containing medium was evaluated for the detection of Escherichia coli in water. A total of 200 typical blue and non-typical blue colonies were isolated from sea and fresh water samples using initial selective enumeration on m-FC agar. Pure cultures of the selected colonies were further tested using the MUG assay and identified using the API 20E method. Of the colonies tested which were shown to be positive using the MUG assay 99.4% were Escherichia coli. The results of this study indicate the combination of the m-FC method followed by the MUG assay to be highly efficient for the selection and confirmation of E. coli from a wide range of environmental waters.

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Formulating biossay data of chemicals and environmental water

Water Science & Technology ◽

10.2166/wst.2000.0367 ◽

2000 ◽

Vol 42 (3-4) ◽

pp. 115-123 ◽

Cited By ~ 8

Author(s):

R. Shoji ◽

A. Sakoda ◽

Y. Sakai ◽

M. Suzuki

Keyword(s):

Water Quality ◽

Low Density Lipoprotein ◽

Water Environment ◽

Density Lipoprotein ◽

Environmental Water ◽

Toxicity Data ◽

Human Beings ◽

Environmental Waters ◽

Bodies Of Water

The quality of environmental waters such as rivers is often deteriorated by various kinds of trace and unidentified chemicals despite the recent development of sewage systems and wastewater treatment technologies. In addition to contamination by particular toxicants, complex toxicity due to multi-component chemicals could be much more serious. The environmental situation in bodies of water in Japan led us to apply bioassays for monitoring the water quality of environmental waters in order to express the direct and potential toxicity to human beings and ecosystems rather than determinating concentrations of particular chemicals. However, problems arose from the fact that bioassays for pharmaceutical purposes generally required complicated, time-consuming, expert procedures. Also, a methodology for feedback of the resultant toxicity data to water environment management has not been established yet. To this end, we developed a novel bioassay based on the low-density lipoprotein (LDL) uptake activity of human hepatoblastoma cells. The assay enabled us to directly detect the toxicity of environmental waters within 4 hours of exposure. This is a significantly quick and easy procedure as compared to that of conventional bioassays. The toxicity data for 255 selected chemicals and environmental waters obtained by this method were organized by a mathematical equation in order to make those data much more effectively and practically useful to the management of environmental waters. Our methodology represents a promising example of applying bioassays to monitor environmental water quality and generating potential solutions to the toxicity problems encountered.

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A novel application of simple submersible yeast-based microbial fuel cells as dissolved oxygen sensors in environmental waters

Enzyme and Microbial Technology ◽

10.1016/j.enzmictec.2021.109831 ◽

2021 ◽

pp. 109831

Author(s):

Marcelinus Christwardana ◽

Linda Aliffia Yoshi ◽

Setyonadi ◽

Ahmad Fudholi

Keyword(s):

Fuel Cells ◽

Dissolved Oxygen ◽

Microbial Fuel Cells ◽

Oxygen Sensors ◽

Environmental Waters

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Multidrug-Resistant Listeria Species Shows Abundance in Environmental Waters of a Key District Municipality in South Africa

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph18020481 ◽

2021 ◽

Vol 18 (2) ◽

pp. 481

Author(s):

Liyabona Mpondo ◽

Kingsley Ehi Ebomah ◽

Anthony Ifeanyi Okoh

Keyword(s):

South Africa ◽

Surface Waters ◽

Multidrug Resistant ◽

Eastern Cape ◽

Environmental Waters ◽

Potential Health ◽

Phenotypic Resistance ◽

Listeria Species ◽

Polymerase Chain ◽

Identification And Characterization

The prevalence of bacteria with multidrug-resistance (MDR) is a significant threat to public health globally. Listeria spp. are naturally ubiquitous, with L. monocytogenes particularly being ranked as important foodborne disease-causing microorganisms. This study aimed to evaluate the incidence and determine the antimicrobial resistance (AMR) profiles of multidrug-resistant Listeria spp. (MDRL) isolated from different environmental samples (river and irrigation water) in the Sarah Baartman District Municipality (SBDM), Eastern Cape Province (ECP), South Africa. Molecular identification and characterization were carried out using polymerase chain reaction (PCR) and isolates that exhibited phenotypic resistance were further screened for relevant antimicrobial-resistant genes (ARGs). Findings revealed a total of 124 presumptive Listeria isolates; 69 were molecularly confirmed Listeria species. Out of the confirmed species, 41 isolates (59%) were classified as L. monocytogenes while 9 (13%) were classified as L. welshimeri. All Listeria spp. exhibited phenotypic resistance against ampicillin, penicillin, and trimethoprim-sulphamethoxazole and further screening revealed ARGs in the following proportions: sulI (71%), blaTEM (66%), tetA (63%), and blaCIT (33%). Results confirmed the occurrence of ARGs among Listeria inhabiting surface waters of ECP. The present study indicates that the river water samples collected from SBDM are highly contaminated with MDRL, hence, constituting a potential health risk.

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