A novel method to measure both the reductive and the radical scavenging activity in a linoleic acid model system

2007 ◽  
Vol 51 (12) ◽  
pp. 1441-1446 ◽  
Author(s):  
Michael Lindenmeier ◽  
Alexander Burkon ◽  
Veronika Somoza
Keyword(s):  
Linoleic Acid ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Model System ◽  
Scavenging Activity ◽  
Novel Method

scholarly journals Anthocyanin-rich purple potato flake extract has antioxidant capacity and improves antioxidant potential in rats

2006 ◽  
Vol 96 (6) ◽  
pp. 1125-1134 ◽  
Author(s):  
Kyu-Ho Han ◽  
Mitsuo Sekikawa ◽  
Ken-ichiro Shimada ◽  
Makoto Hashimoto ◽  
Naoto Hashimoto ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Control Group ◽  
Acid Oxidation ◽  
Mrna Levels ◽  
Scavenging Activity ◽  
Potato Flake ◽  
Linoleic Acid Oxidation ◽  
Potato Flakes

Anthocyanins from various vegetables and fruits have antioxidant activities, however, the bioactivities of coloured potato anthocyanins are not well studied. We examined the antioxidant capacities of pigmented fractions from purple potato flakes in vitro, and the antioxidant potentials of purple potato flakes in vivo. 1,1-Diphenyl-2-picrylhydrazyl radical scavenging activity of the pigmented fraction from Hokkai no. 92 (H92) potato flakes was higher than that from Kitamurasaki (KM) potato flakes. Extracts equivalent to 600 μg pigmented fractions from KM and H92 potato flakes inhibited linoleic acid oxidation in the order trolox>H92 ≥ KM>control. Rats were fed 25 % KM or H92 potato flake diets for 4 weeks. The major anthocyanin was identified as petanin. Control rats were fed a diet with cornstarch instead of potato flakes for 4 weeks. The serum antioxidant potential level in the H92 group was significantly higher than that in the control group. The degree of hepatic lipid peroxidation in the H92 group was significantly lower than that in the control group. Hepatic Cu/Zn-superoxide dismutase (SOD), Mn-SOD and glutathione peroxidase (GSH-Px) mRNA levels in the H92 group were significantly higher than those in the control group. Similar significant differences in Cu/Zn-SOD and Mn-SOD mRNA levels between the KM and control groups were found. The present results suggest that purple potato flakes have antioxidant functions with regard to radical scavenging activity and inhibition of linoleic acid oxidation, and that they improve the antioxidant potentials in rats by enhancing hepatic Mn-SOD, Cu/Zn-SOD and GSH-Px mRNA expression.

Effect of Metal Ions (Cu2+/Fe2+) on the Antioxidant Activity of L-Ascorbic Acid-Glycine Model System

2011 ◽  
Vol 396-398 ◽  
pp. 28-31
Author(s):  
Cheng Yi Lei ◽  
Da Zhai Zhou ◽  
Rong Zhao ◽  
Qi Hui Deng ◽  
Ai Nong Yu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Maillard Reaction ◽  
Antioxidative Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Model System ◽  
Model Systems ◽  
Scavenging Activity ◽  
Metal Concentrations

The influence of Cu2+, Fe2+ on the Maillard reaction were studied by heating L-ascorbic(ASA) and glycine (Gly) solutions adjusted to pH 5 at 120±2°C for 140 min in an oil bath. The presence of metals affected the intensity of browning and intermediate products, as monitored by absorbance at 420 nm and absorbance at 294 nm, sharply increased with the increase of metal concentrations applied (0.000M, 0.005M, 0.010M, 0.015M, 0.020M). Thereafter, slight increases were observed up to 0.020M. Antioxidative activity of all MRPs derived from ASA-Gly model systems sharply increased at 0.015M (P < 0.05) and slightly changes in activity were found with increasing metal concentrations up to 0.020M. Moreover, radical-scavenging activity correlated well with browning intensity and absorbance at 294 nm.

scholarly journals Seasonal Variability of Juniperus communis L. Berry Ethanol Extracts: 1. In Vitro Hydroxyl Radical Scavenging Activity

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4114
Author(s):  
Jozef Fejér ◽  
Ivan Kron ◽  
Daniela Gruľová ◽  
Adriana Eliašová
Keyword(s):  
Hydroxyl Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Total Phenols ◽  
North East ◽  
Hydroxyl Radical Scavenging Activity ◽  
Novel Method ◽  
Ethanol Extracts

In the present study, in vitro hydroxyl radical scavenging activities, total phenols and terpene contents in 70% ethanol extracts were evaluated. Samples of crushed (CB) and non-crushed ripe juniper berries (NCB) collected at five localities in North-East Slovakia during the years 2012–2014 were compared. Standard or modified methods for determining phytochemical and antioxidant activity were used together with a novel method for the evaluation of the results after the correction of the measured values per gram of dry matter (DM). Statistically significantly higher DM contents (average values for three years) were found in the CB extracts (ranging from 18.86 to 21.91 mg/mL) in comparison to those for NCB (ranging from 2.59 to 9.90 mg/mL). Depending on the localities and years, the hydroxyl radical scavenging activity ranged from 15.52 to 32.85% for NCB and from 65.59 to 88.12% for CB, respectively. The contents of total phenols ranged from 43.75 to 246.75 mg gallic acid equivalents (GAE)/L (NCB) and from 151.84 to 278.19 mg GAE/L (CB), respectively. However, the higher content of total phenols per gram of DM was found in the NCB extracts (8.49–42.23 mg GAE/g DM) and then in CB (6.87–18.77 GAE/g DM). The results obtained in this study showed a higher efficiency for extraction from juniper berries in 70% ethanol if the pericarp was disrupted in comparison to that achieved with the maceration of intact berries.

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