scholarly journals Technical and dosimetric realization of in vivo x‐ray microbeam irradiations at the Munich Compact Light Source

2020 ◽  
Vol 47 (10) ◽  
pp. 5183-5193 ◽  
Author(s):  
Karin Burger ◽  
Theresa Urban ◽  
Annique C. Dombrowsky ◽  
Martin Dierolf ◽  
Benedikt Günther ◽  
...  
2020 ◽  
Vol 27 (5) ◽  
pp. 1395-1414 ◽  
Author(s):  
Benedikt Günther ◽  
Regine Gradl ◽  
Christoph Jud ◽  
Elena Eggl ◽  
Juanjuan Huang ◽  
...  

Inverse Compton scattering provides means to generate low-divergence partially coherent quasi-monochromatic, i.e. synchrotron-like, X-ray radiation on a laboratory scale. This enables the transfer of synchrotron techniques into university or industrial environments. Here, the Munich Compact Light Source is presented, which is such a compact synchrotron radiation facility based on an inverse Compton X-ray source (ICS). The recent improvements of the ICS are reported first and then the various experimental techniques which are most suited to the ICS installed at the Technical University of Munich are reviewed. For the latter, a multipurpose X-ray application beamline with two end-stations was designed. The beamline's design and geometry are presented in detail including the different set-ups as well as the available detector options. Application examples of the classes of experiments that can be performed are summarized afterwards. Among them are dynamic in vivo respiratory imaging, propagation-based phase-contrast imaging, grating-based phase-contrast imaging, X-ray microtomography, K-edge subtraction imaging and X-ray spectroscopy. Finally, plans to upgrade the beamline in order to enhance its capabilities are discussed.


Author(s):  
Kaye Morgan ◽  
Regine Gradl ◽  
Martin Dierolf ◽  
Christoph Jud ◽  
Benedikt Günther ◽  
...  

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Regine Gradl ◽  
Martin Dierolf ◽  
Benedikt Günther ◽  
Lorenz Hehn ◽  
Winfried Möller ◽  
...  

Author(s):  
Yue Hu ◽  
Yanmin Yang ◽  
Xiaoxiao Li ◽  
Xin Wang ◽  
Yunqian Li ◽  
...  

Here, we have discovered a X-ray excited long afterglow phosphor β-NaYF4: Tb3+. After the irradiation of X-ray, the green emission can persist for more than 240 h. After 36 h, the afterglow intensity arrived at 0.69 mcd•m-2, which can clearly be observed by naked eyes. Even after 84 h, the afterglow emission brightness still reached 0.087 mcd•m-2. Also, combined with the results of thermoluminescence and photoluminescence, the super long afterglow emission of β-NaYF4: Tb3+ can be ascribed to the tunneling model associated with F centers. More importantly, the super long green afterglow emission of β-NaYF4: Tb3+ has been successfully used as in vivo light source to activate g-C3N4 for photodynamic therapy(PDT)and bacteria destruction. Furthermore, super long persistent luminescence of β-NaYF4: Tb3+ could be repeatedly charged by X-ray for many circulations, which indicates that the phosphors have high photo stability under repeated cycles of alternating X-ray irradiation.


2018 ◽  
Vol 24 (S1) ◽  
pp. 984-985 ◽  
Author(s):  
Benedikt Günther ◽  
Martin Dierolf ◽  
Regine Gradl ◽  
Elena Eggl ◽  
Christoph Jud ◽  
...  

2019 ◽  
Vol 20 (5) ◽  
pp. 1148 ◽  
Author(s):  
Chun-Chen Yang ◽  
Wei-Yun Wang ◽  
Feng-Huei Lin ◽  
Chun-Han Hou

Conventional photodynamic therapy (PDT) is limited by its penetration depth due to the photosensitizer and light source. In this study, we developed X-ray induced photodynamic therapy that applied X-ray as the light source to activate Ce-doped CaCO3 (CaCO3:Ce) to generate an intracellular reactive oxygen species (ROS) for killing cancer cells. The A549 cell line was used as the in vitro and in vivo model to evaluate the efficacy of X-ray-induced CaCO3:Ce. The cell viability significantly decreased and cell cytotoxicity obviously increased with CaCO3:Ce exposure under X-ray irradiation, which is less harmful than radiotherapy in tumor treatment. CaCO3:Ce produced significant ROS under X-ray irradiation and promoted A549 cancer cell death. CaCO3:Ce can enhance the efficacy of X-ray induced PDT, and tumor growth was inhibited in vivo. The blood analysis and hematoxylin and eosin stain (H&E) stain fully supported the safety of the treatment. The mechanisms underlying ROS and CO2 generation by CaCO3:Ce activated by X-ray irradiation to induce cell toxicity, thereby inhibiting tumor growth, is discussed. These findings and advances are of great importance in providing a novel therapeutic approach as an alternative tumor treatment.


2016 ◽  
Vol 23 (5) ◽  
pp. 1137-1142 ◽  
Author(s):  
Elena Eggl ◽  
Martin Dierolf ◽  
Klaus Achterhold ◽  
Christoph Jud ◽  
Benedikt Günther ◽  
...  

While large-scale synchrotron sources provide a highly brilliant monochromatic X-ray beam, these X-ray sources are expensive in terms of installation and maintenance, and require large amounts of space due to the size of storage rings for GeV electrons. On the other hand, laboratory X-ray tube sources can easily be implemented in laboratories or hospitals with comparatively little cost, but their performance features a lower brilliance and a polychromatic spectrum creates problems with beam hardening artifacts for imaging experiments. Over the last decade, compact synchrotron sources based on inverse Compton scattering have evolved as one of the most promising types of laboratory-scale X-ray sources: they provide a performance and brilliance that lie in between those of large-scale synchrotron sources and X-ray tube sources, with significantly reduced financial and spatial requirements. These sources produce X-rays through the collision of relativistic electrons with infrared laser photons. In this study, an analysis of the performance, such as X-ray flux, source size and spectra, of the first commercially sold compact light source, the Munich Compact Light Source, is presented.


2018 ◽  
Vol 24 (S2) ◽  
pp. 352-353
Author(s):  
Regine Gradl ◽  
Martin Dierolf ◽  
Lorenz Hehn ◽  
Benedikt Gunther ◽  
David Kutschke ◽  
...  

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