In vivo x-ray imaging of the respiratory system using synchrotron sources and a compact light source

Inverse Compton scattering provides means to generate low-divergence partially coherent quasi-monochromatic, i.e. synchrotron-like, X-ray radiation on a laboratory scale. This enables the transfer of synchrotron techniques into university or industrial environments. Here, the Munich Compact Light Source is presented, which is such a compact synchrotron radiation facility based on an inverse Compton X-ray source (ICS). The recent improvements of the ICS are reported first and then the various experimental techniques which are most suited to the ICS installed at the Technical University of Munich are reviewed. For the latter, a multipurpose X-ray application beamline with two end-stations was designed. The beamline's design and geometry are presented in detail including the different set-ups as well as the available detector options. Application examples of the classes of experiments that can be performed are summarized afterwards. Among them are dynamic in vivo respiratory imaging, propagation-based phase-contrast imaging, grating-based phase-contrast imaging, X-ray microtomography, K-edge subtraction imaging and X-ray spectroscopy. Finally, plans to upgrade the beamline in order to enhance its capabilities are discussed.

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Technical and dosimetric realization of in vivo x‐ray microbeam irradiations at the Munich Compact Light Source

Medical Physics ◽

10.1002/mp.14433 ◽

2020 ◽

Vol 47 (10) ◽

pp. 5183-5193 ◽

Cited By ~ 1

Author(s):

Karin Burger ◽

Theresa Urban ◽

Annique C. Dombrowsky ◽

Martin Dierolf ◽

Benedikt Günther ◽

...

Keyword(s):

Light Source ◽

X Ray ◽

Compact Light Source

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Dynamic X-ray Imaging at the Munich Compact Light Source

Microscopy and Microanalysis ◽

10.1017/s1431927618014046 ◽

2018 ◽

Vol 24 (S2) ◽

pp. 352-353

Author(s):

Regine Gradl ◽

Martin Dierolf ◽

Lorenz Hehn ◽

Benedikt Gunther ◽

David Kutschke ◽

...

Keyword(s):

Light Source ◽

X Ray ◽

X Ray Imaging ◽

Compact Light Source

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Propagation-based Phase-Contrast X-ray Imaging at a Compact Light Source

Scientific Reports ◽

10.1038/s41598-017-04739-w ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 29

Author(s):

Regine Gradl ◽

Martin Dierolf ◽

Lorenz Hehn ◽

Benedikt Günther ◽

Ali Önder Yildirim ◽

...

Keyword(s):

Light Source ◽

Phase Contrast ◽

X Ray ◽

X Ray Imaging ◽

Compact Light Source

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Super Long Green Persistent Luminescence from X-Ray Excited β-NaYF4: Tb3+

10.20944/preprints201902.0241.v1 ◽

2019 ◽

Cited By ~ 1

Author(s):

Yue Hu ◽

Yanmin Yang ◽

Xiaoxiao Li ◽

Xin Wang ◽

Yunqian Li ◽

...

Keyword(s):

Photodynamic Therapy ◽

Light Source ◽

Green Emission ◽

Persistent Luminescence ◽

Tunneling Model ◽

X Ray ◽

Long Afterglow ◽

Photo Stability ◽

F Centers

Here, we have discovered a X-ray excited long afterglow phosphor β-NaYF4: Tb3+. After the irradiation of X-ray, the green emission can persist for more than 240 h. After 36 h, the afterglow intensity arrived at 0.69 mcd•m-2, which can clearly be observed by naked eyes. Even after 84 h, the afterglow emission brightness still reached 0.087 mcd•m-2. Also, combined with the results of thermoluminescence and photoluminescence, the super long afterglow emission of β-NaYF4: Tb3+ can be ascribed to the tunneling model associated with F centers. More importantly, the super long green afterglow emission of β-NaYF4: Tb3+ has been successfully used as in vivo light source to activate g-C3N4 for photodynamic therapy（PDT）and bacteria destruction. Furthermore, super long persistent luminescence of β-NaYF4: Tb3+ could be repeatedly charged by X-ray for many circulations, which indicates that the phosphors have high photo stability under repeated cycles of alternating X-ray irradiation.

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The Munich Compact Light Source: Biomedical Research At a Laboratory-Scale Inverse-Compton Synchrotron X-ray Source

Microscopy and Microanalysis ◽

10.1017/s143192761800541x ◽

2018 ◽

Vol 24 (S1) ◽

pp. 984-985 ◽

Cited By ~ 4

Author(s):

Benedikt Günther ◽

Martin Dierolf ◽

Regine Gradl ◽

Elena Eggl ◽

Christoph Jud ◽

...

Keyword(s):

Light Source ◽

Biomedical Research ◽

Laboratory Scale ◽

X Ray ◽

Inverse Compton ◽

Compact Light Source

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Rare-Earth-Doped Calcium Carbonate Exposed to X-ray Irradiation to Induce Reactive Oxygen Species for Tumor Treatment

International Journal of Molecular Sciences ◽

10.3390/ijms20051148 ◽

2019 ◽

Vol 20 (5) ◽

pp. 1148 ◽

Cited By ~ 1

Author(s):

Chun-Chen Yang ◽

Wei-Yun Wang ◽

Feng-Huei Lin ◽

Chun-Han Hou

Keyword(s):

Reactive Oxygen Species ◽

Photodynamic Therapy ◽

Tumor Growth ◽

Light Source ◽

Reactive Oxygen ◽

Blood Analysis ◽

Oxygen Species ◽

Tumor Treatment ◽

X Ray

Conventional photodynamic therapy (PDT) is limited by its penetration depth due to the photosensitizer and light source. In this study, we developed X-ray induced photodynamic therapy that applied X-ray as the light source to activate Ce-doped CaCO3 (CaCO3:Ce) to generate an intracellular reactive oxygen species (ROS) for killing cancer cells. The A549 cell line was used as the in vitro and in vivo model to evaluate the efficacy of X-ray-induced CaCO3:Ce. The cell viability significantly decreased and cell cytotoxicity obviously increased with CaCO3:Ce exposure under X-ray irradiation, which is less harmful than radiotherapy in tumor treatment. CaCO3:Ce produced significant ROS under X-ray irradiation and promoted A549 cancer cell death. CaCO3:Ce can enhance the efficacy of X-ray induced PDT, and tumor growth was inhibited in vivo. The blood analysis and hematoxylin and eosin stain (H&E) stain fully supported the safety of the treatment. The mechanisms underlying ROS and CO2 generation by CaCO3:Ce activated by X-ray irradiation to induce cell toxicity, thereby inhibiting tumor growth, is discussed. These findings and advances are of great importance in providing a novel therapeutic approach as an alternative tumor treatment.

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Visualizing treatment delivery and deposition in mouse lungs using in vivo x-ray imaging

Journal of Controlled Release ◽

10.1016/j.jconrel.2019.06.035 ◽

2019 ◽

Vol 307 ◽

pp. 282-291 ◽

Cited By ~ 11

Author(s):

Regine Gradl ◽

Martin Dierolf ◽

Lin Yang ◽

Lorenz Hehn ◽

Benedikt Günther ◽

...

Keyword(s):

Treatment Delivery ◽

X Ray ◽

X Ray Imaging ◽

Mouse Lungs

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Development of a Mercuric Iodide Detector Array For In-Vivo X-Ray Imaging

Advances in X-ray Analysis ◽

10.1154/s0376030800018310 ◽

1994 ◽

Vol 38 ◽

pp. 615-624

Author(s):

Bradley E. Patt ◽

Jan S. Iwanczyk ◽

Martin P. Tornai ◽

Craig S. Levin ◽

Edward J. Hoffman

Keyword(s):

Gamma Camera ◽

Gamma Ray ◽

Active Area ◽

Detector Array ◽

Mercuric Iodide ◽

X Ray ◽

X Ray Imaging ◽

Spatial Performance ◽

Gamma Ray Imaging

Abstract A nineteen element mercuric iodide (HgI2) detector array has been developed in order to investigate the potential of using this technology for in-vivo x-ray and gamma-ray imaging. A prototype cross-grid detector array was constructed with hexagonal pixels of 1.9 mm diameter (active area = 3.28 mm2) and 0.2 mm thick septa. The overall detector active area is roughly 65 mm2. A detector thickness of 1.2 mm was used to achieve about 100% efficiency at 60 keV and 67% efficiency at 140 keV The detector fabrication, geometry and structure were optimized for charge collection and to minimize crosstalk between elements. A section of a standard high resolution cast-lead gamma-camera collimator was incorporated into the detector to provide collimation matching the discrete pixel geometry. Measurements of spectral and spatial performance of the array were made using 241-Am and 99m-Tc sources. These measurements were compared with similar measurements made using an optimized single HgI2 x-ray detector with active area of about 3 mm2 and thickness of 500 μm.

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