Evidence for a binding site on the sperm plasma membrane which recognizes the murine zona pellucida: A binding site on the sperm plasma membrane

1986 ◽  
Vol 14 (3) ◽  
pp. 235-243 ◽  
Author(s):  
Gary R. Poirier ◽  
Roderick Robinson ◽  
Richard Richardson ◽  
Kathy Hinds ◽  
Deborah Clayton
Keyword(s):  
Plasma Membrane ◽  
Binding Site ◽  
Zona Pellucida ◽  
Sperm Plasma Membrane

scholarly journals Glycodelin-A interacts with fucosyltransferase on human sperm plasma membrane to inhibit spermatozoa-zona pellucida binding

2006 ◽  
Vol 120 (1) ◽  
pp. 33-44 ◽  
Author(s):  
P. C. N. Chiu ◽  
M.-K. Chung ◽  
R. Koistinen ◽  
H. Koistinen ◽  
M. Seppala ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Zona Pellucida ◽  
Human Sperm ◽  
Sperm Plasma Membrane ◽  
Glycodelin A

The interaction of living boar sperm and sperm plasma membrane vesicles with the porcine zona pellucida

1981 ◽  
Vol 84 (1) ◽  
pp. 144-156 ◽  
Author(s):  
R.N. Peterson ◽  
L.D. Russell ◽  
D. Bundman ◽  
M. Conway ◽  
M. Freund
Keyword(s):  
Plasma Membrane ◽  
Zona Pellucida ◽  
Membrane Vesicles ◽  
Plasma Membrane Vesicles ◽  
Boar Sperm ◽  
Sperm Plasma Membrane

scholarly journals Update on mammalian sperm capacitation: how much does the horse differ from other species?

Reproduction ◽  
2019 ◽  
Vol 157 (5) ◽  
pp. R181-R197 ◽  
Author(s):  
Bart Leemans ◽  
Tom A E Stout ◽  
Catharina De Schauwer ◽  
Sonia Heras ◽  
Hilde Nelis ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Zona Pellucida ◽  
Mammalian Species ◽  
Cumulus Cells ◽  
Sperm Cells ◽  
Cholesterol Depletion ◽  
Sperm Capacitation ◽  
Sperm Plasma Membrane ◽  
Species Specific

In contrast to various other mammalian species, conventional in vitro fertilization (IVF) with horse gametes is not reliably successful. In particular, stallion spermatozoa fails to penetrate the zona pellucida, most likely due to incomplete activation of stallion spermatozoa (capacitation) under in vitro conditions. In other mammalian species, specific capacitation triggers have been described; unfortunately, none of these is able to induce full capacitation in stallion spermatozoa. Nevertheless, knowledge of capacitation pathways and their molecular triggers might improve our understanding of capacitation-related events observed in stallion sperm. When sperm cells are exposed to appropriate capacitation triggers, several molecular and biochemical changes should be induced in the sperm plasma membrane and cytoplasm. At the level of the sperm plasma membrane, (1) an increase in membrane fluidity, (2) cholesterol depletion and (3) lipid raft aggregation should occur consecutively; the cytoplasmic changes consist of protein tyrosine phosphorylation and elevated pH, cAMP and Ca2+ concentrations. These capacitation-related events enable the switch from progressive to hyperactivated motility of the sperm cells, and the induction of the acrosome reaction. These final capacitation triggers are indispensable for sperm cells to migrate through the viscous oviductal environment, penetrate the cumulus cells and zona pellucida and, finally, fuse with the oolemma. This review will focus on molecular aspects of sperm capacitation and known triggers in various mammalian species. Similarities and differences with the horse will be highlighted to improve our understanding of equine sperm capacitation/fertilizing events.

Sperm-egg interaction: evidence for boar sperm plasma membrane receptors for porcine zona pellucida

Science ◽  
1980 ◽  
Vol 207 (4426) ◽  
pp. 73-74 ◽  
Author(s):  
R. Peterson ◽  
L Russell ◽  
D Bundman ◽  
M Freund
Keyword(s):  
Plasma Membrane ◽  
Zona Pellucida ◽  
Membrane Receptors ◽  
Boar Sperm ◽  
Sperm Plasma Membrane ◽  
Plasma Membrane Receptors

Further characterization of boar sperm plasma membrane proteins with affinity for the porcine zona pellucida

1985 ◽  
Vol 12 (1) ◽  
pp. 91-100 ◽  
Author(s):  
R. N. Peterson ◽  
L. Henry ◽  
W. Hunt ◽  
N. Saxena ◽  
L. D. Russell
Keyword(s):  
Plasma Membrane ◽  
Membrane Proteins ◽  
Zona Pellucida ◽  
Plasma Membrane Proteins ◽  
Boar Sperm ◽  
Sperm Plasma Membrane

scholarly journals Role of actin cytoskeleton in mammalian sperm capacitation and the acrosome reaction

Reproduction ◽  
2005 ◽  
Vol 129 (3) ◽  
pp. 263-268 ◽  
Author(s):  
Haim Breitbart ◽  
Gili Cohen ◽  
Sara Rubinstein
Keyword(s):  
Plasma Membrane ◽  
Zona Pellucida ◽  
Acrosome Reaction ◽  
Reproductive Tract ◽  
Female Reproductive Tract ◽  
Mammalian Sperm ◽  
Acrosomal Membrane ◽  
Close Proximity ◽  
Sperm Plasma Membrane ◽  
To Come

In order to fertilize, the mammalian spermatozoa should reside in the female reproductive tract for several hours, during which they undergo a series of biochemical modifications collectively called capacitation. Only capacitated sperm can undergo the acrosome reaction after binding to the egg zona pellucida, a process which enables sperm to penetrate into the egg and fertilize it. Polymerization of globular (G)-actin to filamentous (F)-actin occurs during capacitation, depending on protein kinase A activation, protein tyrosine phosphorylation, and phospholipase D activation. F-actin formation is important for the translocation of phospholipase C from the cytosol to the sperm plasma membrane during capacitation. Prior to the occurrence of the acrosome reaction, the F-actin should undergo depolymerization, a necessary process which enables the outer acrosomal membrane and the overlying plasma membrane to come into close proximity and fuse. The binding of the capacitated sperm to the zona pellucida induces a fast increase in sperm intracellular calcium, activation of actin severing proteins which break down the actin fibers, and allows the acrosome reaction to take place.

Behaviour and role of an intra-acrosomal antigenic molecule, acrin 3, during mouse fertilisation in vitro

Zygote ◽  
2000 ◽  
Vol 8 (4) ◽  
pp. 329-338 ◽  
Author(s):  
D.K. Saxena ◽  
I. Tanii ◽  
T. Oh-oka ◽  
K. Yoshinaga ◽  
K. Toshimori
Keyword(s):  
Monoclonal Antibody ◽  
Plasma Membrane ◽  
Zona Pellucida ◽  
Acrosome Reaction ◽  
Formation Rate ◽  
Dependent Manner ◽  
Sperm Plasma Membrane ◽  
Dose Dependent

In this study we examined the behaviour and role of an intra-acrosomal antigenic molecule, acrin 3, during mouse fertilisation in vitro by assessing the effect of its pertinent monoclonal antibody mMC101. Experiments were designed to assess the effect of mMC101 on sperm–zona pellucida binding, the acrosome reaction, zona pellucida penetration, sperm–egg fusion, and fertilisation in vitro. mMC101 did not affect sperm motility or primary and secondary binding to the zona pellucida, but significantly inhibited fertilisation of zona-pellucida-intact oocytes in a dose-dependent manner. In the presence of mMC101 at 100 μg/ml concentration in TYH medium, none of the oocytes developed to pronuclear stage by 5 h after co-incubation of the gametes, but the pronucleus formation rate recovered to some extent (45.3%) after 8 h, indicating a delay of early embryonic development. mMC101 also delayed and significantly suppressed zona pellucida penetration by sperm. Acrin 3 dispersed and did not remain on completely acrosome-reacted sperm. Although mMC101 did not influence the zona-pellucida-induced acrosome reaction, it significantly inhibited fertilisation when acrosome-reacted sperm in the presence of mMC101 inseminated zona-pellucida-free oocytes. However, fertilisation remained unaffected when acrosome-reacted sperm in the absence of mMC101 inseminated zona-pellucida-free oocytes even in its presence. Thus, acrin 3 appears to facilitate zona pellucida penetration and is also likely to be involved in sperm–oocyte fusion by modifying the sperm plasma membrane during the acrosome reaction.

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